lambda embl4 ( (Agilent technologies)

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Agilent technologies lambda embl4 (
Lambda Embl4 (, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+embl4/pm10483738-40-22-24
Average 90 stars, based on 1 article reviews

lambda embl4 ( - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Sequence and transcriptional studies of five clustered flagellin genes from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1.
Article Snippet: .. A lambda phage library of P. kodakaraensis KOD1 was constructed by ligating genomic DNA partially digested with EcoRI into EcoRI-digested arms of lambda EMBL4 (Stratagene, La Jolla, CA, USA). .. The positive phage clone was screened by plaque hybridization using the PCR product as a probe according to the DIG DNA Labeling kit (Boehringer Mannheim, Mannheim, Germany).

other:

Article Title: Analysis of the Disease Potential of a Recombinant Retrovirus Containing Friend Murine Leukemia Virus Sequences and a Unique Long Terminal Repeat from Feline Leukemia Virus
Article Snippet: The DNA was digested with Eco RI and ligated to Eco RI-digested λ phage EMBL-4 (Stratagene, La Jolla, Calif.), and a DNA library was constructed.

Article Title: Cyclin D1 is necessary but not sufficient for anchorage-independent growth of rat mammary tumor cells and is associated with resistance of the Copenhagen rat to mammary carcinogenesis.
Article Snippet: High molecular weight DNA from a cell line we designated FA4.4 was partially digested with EcoRI and ligated to EcoRI arms of the bacteriophage vector EMBL-4 (Stratagene, La Jolla, CA, USA), as described by the company.

Article Title: Induction of Disease by a Molecularly Cloned Highly Pathogenic Simian Immunodeficiency Virus/Human Immunodeficiency Virus Chimera Is Multigenic
Article Snippet: Accordingly, Hirt-fractionated ( 9 ), SHIV DH12R-PS1 -infected monkey PBMC DNA was digested with EcoRI and ligated to similarly cleaved lambda phage EMBL-4 DNA (Stratagene/Biocrest; Cedar Creek, Tex.) as previously described ( 22 ).

Article Title: Pk-cdcA encodes a CDC48/VCP homolog in the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1: transcriptional and enzymatic characterization.
Article Snippet: A gene encoding a cell division control protein from the hyperthermophilic archaeon Pyrococcus kodakaraensisKOD1, Pk-cdcA, was cloned and sequenced.. The Pk-cdcA gene is composed of 2508 nucleotides, encoding a protein of 835 amino acids with a molecular mass of 93,666 Da.. Pk-CdcA has a typical Walker-type ATPase motif and was classi®ed as a new member of the CDC48/VCP subfamily of so-called AAA proteins.

Clone Assay:

Article Title: Complete sequence, gene arrangement, and genetic code of mitochondrial DNA of the cephalochordate Branchiostoma floridae (Amphioxus)
Article Snippet: .. The entire Branchiostoma mtDNA was then cloned into the lambda vector EMBL4 (Stratagene) using the unique EcoRI site at position 8,834–8,839 (numbering from the first nucleotide of COI; see figs. 1 and 2). ..

Plasmid Preparation:

In Vitro:

Article Title: Chromosome DNA coding for human hepatocyte growth factor
Article Snippet: After dialysis against TE buffer at 4° C. overnight, the obtained fraction was precipitated with ethanol and dissolved in 1 ml of TE buffer. (2) Preparation of vector DNA Phage vector arms were prepared in accordance with the method of Maniatis et al. [Cell, 15, 687 (1978)] as follows: Charon 4A phage DNA (50 μg) completely digested with the restriction enzyme EcoRI [Science, 196, 161 (1977)] was treated in a TE buffer containing 10 mM MgCl2 at 42° C. for 1 hour and then subjected to sucrose density gradient centrifugation in the same manner as (1) above, and the fraction containing both the left and right arms was dissolved in 100 μl of TE buffer. .. EMBL3 and EMBL4 phage vectors were purchased from Stratagene. (3) Preparation of chromosome DNA libraries The chromosome DNA obtained in (1) above (3 μg) and 1 μg of the phage vector DNA obtained in (2) above were ligated to each other using T4 DNA ligase, and the solution was subjected to in vitro packaging reaction using the in vitro packaging kit Giga Pack Gold (Stratagene). ..

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