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iscript reverse transcriptase cdna synthesis kit  (Bio-Rad)


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    Structured Review

    Bio-Rad iscript reverse transcriptase cdna synthesis kit
    Iscript Reverse Transcriptase Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 49483 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase/iScript+cDNA+Synthesis+Kit/pmc13080255-182-23-29
    Average 99 stars, based on 49483 article reviews
    iscript reverse transcriptase cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC
    Article Snippet: The RNA purity was measured by NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA). .. One μg of RNA was reverse-transcribed into cDNA using the iScript cDNA Synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). .. Target gene expression ( ) was assessed by qRT-PCR reaction containing iTaq Universal SYBR Green Supermix (Bio-Rad). qRT-PCR was performed using the QuantStudio 3 real-time PCR system (Thermo Fisher Scientific).

    Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration
    Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted using TRIzol reagent (Bio-Rad, USA). .. Extracted mRNA was reverse transcribed into cDNA using the iScript1TM cDNA synthesis kit (Bio-Rad, USA), followed by real-time PCR (RT-PCR) using TB Green Supermix (iTaq Universal SYBR Green Supermix, Bio-Rad, USA) according to the manufacturer's instructions (n = 3). ..

    Article Title: Time- and spatially resolved LNA delivery via thermally controlled SPION technology
    Article Snippet: .. For quantitative detection of mRNAs, reverse transcription of total RNA prior to real-time qPCR was performed using the iScript Select cDNA synthesis kit (Bio-Rad), according to the manufacturer’s instructions. .. Real-time qPCR was performed in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using specific primers ( ) and the iQ SYBR Green Mix (Bio-Rad), according to the manufacturer’s protocol.

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    cDNA Synthesis:

    Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC
    Article Snippet: The RNA purity was measured by NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA). .. One μg of RNA was reverse-transcribed into cDNA using the iScript cDNA Synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). .. Target gene expression ( ) was assessed by qRT-PCR reaction containing iTaq Universal SYBR Green Supermix (Bio-Rad). qRT-PCR was performed using the QuantStudio 3 real-time PCR system (Thermo Fisher Scientific).

    Article Title: Hyperacetylation escalates myocardial susceptibility to ischemia reperfusion injury by mediating mitochondrial supercomplexes assembly in T2DM
    Article Snippet: The signals were quantitatively analyzed with ImageJ software (NIH), and the results were normalized to the GAPDH protein levels. .. Total RNA was extracted from heart tissues or cultured cardiomyocytes using TRIzol (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Inc.) according to the manufacturer's guidelines. .. Real-time quantitative PCR was performed using 2 × iTaqTM Universal SYBR-Green Supermix (Bio-Rad Laboratories, Inc.) on a CFX Connect Real-time PCR Detection system (Bio-Rad Laboratories, Inc.).

    Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration
    Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted using TRIzol reagent (Bio-Rad, USA). .. Extracted mRNA was reverse transcribed into cDNA using the iScript1TM cDNA synthesis kit (Bio-Rad, USA), followed by real-time PCR (RT-PCR) using TB Green Supermix (iTaq Universal SYBR Green Supermix, Bio-Rad, USA) according to the manufacturer's instructions (n = 3). ..

    Article Title: Time- and spatially resolved LNA delivery via thermally controlled SPION technology
    Article Snippet: .. For quantitative detection of mRNAs, reverse transcription of total RNA prior to real-time qPCR was performed using the iScript Select cDNA synthesis kit (Bio-Rad), according to the manufacturer’s instructions. .. Real-time qPCR was performed in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using specific primers ( ) and the iQ SYBR Green Mix (Bio-Rad), according to the manufacturer’s protocol.

    Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1
    Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An iScript gDNA Clear cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to convert RNA to cDNA, which was amplified using PowerTrack SYBR Green MasterMix (Applied Biosystems, Waltham, MA). qPCR was performed in QuantStudio 3 thermal cycler (Applied Biosystems). ..

    Article Title: TNKS1 mediates the PTEN-PI3K/AKT pathway to regulate glycolysis and proliferation in gliomas
    Article Snippet: After 24 h of drug treatment, total RNA was extracted from cells using TRIzol reagent (15596026, Invitrogen, Carlsbad, CA, USA). .. Complementary DNA (cDNA) was then transcribed using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). .. PCR was performed using SYBR Green Real-Time PCR Master Mix (TOYOBO, Tokyo, Japan).

    Article Title: Bone matrix-inspired whitlockite porous ceramic with effects of autophagy activation contributes to bone regeneration
    Article Snippet: TRIZOL (Thermo Fisher Scientific, USA) reagent was implemented to get total RNA as manufacturer guidelines. .. Conversion of total RNA to complementary DNA (cDNA) was carried out using an iScript cDNA synthesis kit (Bio-RAD, USA). .. Subsequently, a SoFast EvaGreen Supermix kit (Bio-RAD, USA) on a CFX96 real-time PCR detection platform (Bio-RAD, USA) was utilized to perform the quantitative PCR.

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    Cell Culture:

    Article Title: Hyperacetylation escalates myocardial susceptibility to ischemia reperfusion injury by mediating mitochondrial supercomplexes assembly in T2DM
    Article Snippet: The signals were quantitatively analyzed with ImageJ software (NIH), and the results were normalized to the GAPDH protein levels. .. Total RNA was extracted from heart tissues or cultured cardiomyocytes using TRIzol (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Inc.) according to the manufacturer's guidelines. .. Real-time quantitative PCR was performed using 2 × iTaqTM Universal SYBR-Green Supermix (Bio-Rad Laboratories, Inc.) on a CFX Connect Real-time PCR Detection system (Bio-Rad Laboratories, Inc.).

    Synthesized:

    Article Title: Hyperacetylation escalates myocardial susceptibility to ischemia reperfusion injury by mediating mitochondrial supercomplexes assembly in T2DM
    Article Snippet: The signals were quantitatively analyzed with ImageJ software (NIH), and the results were normalized to the GAPDH protein levels. .. Total RNA was extracted from heart tissues or cultured cardiomyocytes using TRIzol (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Inc.) according to the manufacturer's guidelines. .. Real-time quantitative PCR was performed using 2 × iTaqTM Universal SYBR-Green Supermix (Bio-Rad Laboratories, Inc.) on a CFX Connect Real-time PCR Detection system (Bio-Rad Laboratories, Inc.).

    Real-time Polymerase Chain Reaction:

    Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration
    Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted using TRIzol reagent (Bio-Rad, USA). .. Extracted mRNA was reverse transcribed into cDNA using the iScript1TM cDNA synthesis kit (Bio-Rad, USA), followed by real-time PCR (RT-PCR) using TB Green Supermix (iTaq Universal SYBR Green Supermix, Bio-Rad, USA) according to the manufacturer's instructions (n = 3). ..

    Article Title: Time- and spatially resolved LNA delivery via thermally controlled SPION technology
    Article Snippet: .. For quantitative detection of mRNAs, reverse transcription of total RNA prior to real-time qPCR was performed using the iScript Select cDNA synthesis kit (Bio-Rad), according to the manufacturer’s instructions. .. Real-time qPCR was performed in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using specific primers ( ) and the iQ SYBR Green Mix (Bio-Rad), according to the manufacturer’s protocol.

    Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1
    Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An iScript gDNA Clear cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to convert RNA to cDNA, which was amplified using PowerTrack SYBR Green MasterMix (Applied Biosystems, Waltham, MA). qPCR was performed in QuantStudio 3 thermal cycler (Applied Biosystems). ..

    SYBR Green Assay:

    Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration
    Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted using TRIzol reagent (Bio-Rad, USA). .. Extracted mRNA was reverse transcribed into cDNA using the iScript1TM cDNA synthesis kit (Bio-Rad, USA), followed by real-time PCR (RT-PCR) using TB Green Supermix (iTaq Universal SYBR Green Supermix, Bio-Rad, USA) according to the manufacturer's instructions (n = 3). ..

    Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1
    Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An iScript gDNA Clear cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to convert RNA to cDNA, which was amplified using PowerTrack SYBR Green MasterMix (Applied Biosystems, Waltham, MA). qPCR was performed in QuantStudio 3 thermal cycler (Applied Biosystems). ..

    Amplification:

    Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1
    Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An iScript gDNA Clear cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to convert RNA to cDNA, which was amplified using PowerTrack SYBR Green MasterMix (Applied Biosystems, Waltham, MA). qPCR was performed in QuantStudio 3 thermal cycler (Applied Biosystems). ..

    Quantitative RT-PCR:

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.



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