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Amersham Pharmacia Biotech Ltd imagequant data analysis software
Imagequant Data Analysis Software, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagequant+data+analysis+software/imagequant+software/us10208342-1258-66-70
Average 90 stars, based on 1 article reviews
imagequant data analysis software - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Methods of using anti-MIR126 compounds
Article Snippet: Protein levels were determined by densitometry using Image-Quant software (Amersham Pharmacia Biotech, Piscataway, N.J.).

Article Title: Assessment of the role of transcript for GATA-4 as a marker of unfavorable outcome in human adrenocortical neoplasms
Article Snippet: Densitometric analysis was carried out using ImageQuant 2.1 software (Amersham Pharmacia Biotech, Piscataway, NJ).

Software:

Article Title: Pathways and genes differentially expressed in the motor cortex of patients with sporadic amyotrophic lateral sclerosis
Article Snippet: Immunoblots were visualized using the Enhanced Chemiluminescence System (Amersham Pharmacia Biotech). .. Band intensities were determined as background-corrected volume measurements using the ImageQuant TL software (Amersham Pharmacia Biotech), were equalized using CANX as a loading reference (which has not been linked to ALS and in our microarray analysis showed an mRNA ratio diseased vs. control of 0.97), and were subjected to statistical analysis using a two-tailed, heteroscedastic Student's t-test in Excel (Microsoft Corp.). ..

Article Title: The chemokine receptor CXCR4 and its ligand CXCL12 are activated during implantation and placentation in sheep
Article Snippet: .. ECL signals were detected with a Storm imager and quantified with ImageQuant TL software (Amersham Pharmacia Biotech). .. An anti-actin antibody (sc-47778; Santa Cruz Biotechnology) was used at 1:1000 dilution for all Western blots to further demonstrate equal loading.

Article Title: Compounds and methods for treating influenza
Article Snippet: After cell lysis in RIPA buffer (150 mM NaCl, 10 mM Tris-HCl pH 7.5, 4 mM EDTA, 1% Triton X-100, 600 mM KCl), containing 1 mM phenylmethylsulphonyl fluoride (PMSF) and a protease inhibitor cocktail (PIC; Roche Diagnostics GmbH), samples containing the same amount of radioactivity were separated by SDS/PAGE (3% stacking gel, 10% resolving gel) and processed for autoradiography, as described. .. Autoradiographic patterns were visualized and quantified in Typhoon-8600 Imager (Molecular Dynamics, Amersham Pharmacia Biotech) and images were acquired using ImageQuant software (Amersham Pharmacia Biotech) (MDP analysis). ..

Article Title: Deposition of nonsarcomeric alpha-actinin in cardiomyocytes from patients with dilated cardiomyopathy or chronic pressure overload
Article Snippet: .. Quantification was performed by densitometry, either using the gel-analyzing program Quantity One (Protein Imageware System Inc, USA) or scanning the immunoblots on a STORM 860 imager (Amersham Pharmacia Biotech, Germany) using ImageQuant software. ..

Microarray:

Article Title: Pathways and genes differentially expressed in the motor cortex of patients with sporadic amyotrophic lateral sclerosis
Article Snippet: Immunoblots were visualized using the Enhanced Chemiluminescence System (Amersham Pharmacia Biotech). .. Band intensities were determined as background-corrected volume measurements using the ImageQuant TL software (Amersham Pharmacia Biotech), were equalized using CANX as a loading reference (which has not been linked to ALS and in our microarray analysis showed an mRNA ratio diseased vs. control of 0.97), and were subjected to statistical analysis using a two-tailed, heteroscedastic Student's t-test in Excel (Microsoft Corp.). ..

Control:

Article Title: Pathways and genes differentially expressed in the motor cortex of patients with sporadic amyotrophic lateral sclerosis
Article Snippet: Immunoblots were visualized using the Enhanced Chemiluminescence System (Amersham Pharmacia Biotech). .. Band intensities were determined as background-corrected volume measurements using the ImageQuant TL software (Amersham Pharmacia Biotech), were equalized using CANX as a loading reference (which has not been linked to ALS and in our microarray analysis showed an mRNA ratio diseased vs. control of 0.97), and were subjected to statistical analysis using a two-tailed, heteroscedastic Student's t-test in Excel (Microsoft Corp.). ..

Polymerase Chain Reaction:

Article Title: Ameliorating nervous systems disorders
Article Snippet: .. PCR products are electrophoresed on 10% polyacrylamide gels and quantified using the Phosphorimaging system and IMAGEQUANT (Molecular Dynamics, Amersham Pharmacia Biotech, Piscataway, N.J.). ..

Western Blot:

Article Title: Deposition of nonsarcomeric alpha-actinin in cardiomyocytes from patients with dilated cardiomyopathy or chronic pressure overload
Article Snippet: .. Quantification was performed by densitometry, either using the gel-analyzing program Quantity One (Protein Imageware System Inc, USA) or scanning the immunoblots on a STORM 860 imager (Amersham Pharmacia Biotech, Germany) using ImageQuant software. ..



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Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using <t>ImageQuanT</t> data analysis software (Molecular Dynamics)
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Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)

Journal: Cancer immunology, immunotherapy : CII

Article Title: Receptor desensitization and blockade of the suppressive effects of prostaglandin E 2 and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes

doi: 10.1007/s00262-010-0924-z

Figure Lengend Snippet: Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)

Article Snippet: Films were scanned and analyzed by ImageQuanT data analysis software (Molecular Dynamics) [ 17 ].

Techniques: Phospho-proteomics, Incubation, Mass Spectrometry, SDS Page, Software