high stringency buffer (Proteintech)
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Proteintech
high stringency buffer
High Stringency Buffer, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+stringency+buffer/PRDM10+Antibody/pmc12354899-401-6-38
Average 96 stars, based on 1711 article reviews
High Stringency Buffer, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+stringency+buffer/PRDM10+Antibody/pmc12354899-401-6-38
Average 96 stars, based on 1711 article reviews
high stringency buffer - by Bioz Stars,
2026-09
96/100 stars
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Incubation:Article Title: Tet2 deficiency–induced expansion of monocyte-derived macrophages promotes liver fibrosis Article Snippet: .. For each RIP, 50 μl lysate was diluted in 450 μl dilution buffer (0.01% SDS, 1.1% Triton X-100, 167 mM NaCl, and 16.7 mM Article Title: UBR5 regulates the progression of colorectal cancer cells through Snail-induced epithelial–mesenchymal transition Article Snippet: The membranes were probed with primary antibodies, including Flag (Proteintech, Wuhan, China, 66008-4-Ig), Myc (Proteintech, 60003-2-Ig), UBR5 (Proteintech, 66937-1-Ig), Snail (Santa Cruz Biotechnology, Oregon, USA, 166476), phosphorylated Snail (Biodragon, BD-PP0568), Slug (Santa Cruz Biotechnology, 271977), E-cadherin (Proteintech, 20874-1-AP), N-cadherin (BD Transduction Laboratories, Franklin Lakes, USA, 610920), GSK3β (Proteintech, 82061-1-RR), pGSK3β (Proteintech, 67558-1-Ig), green fluorescent protein (GFP; Proteintech, 66002-1-Ig), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Bioss, Woburn, USA, 0978M). .. For the immunoprecipitation assay, HEK293T cells were co-transfected with the indicated plasmids and treated with 10 μM proteasome inhibitor MG132 (Sigma–Aldrich, St. Louis, USA, C2211) for 6 h, cells were lysed, and the supernatants were incubated with Article Title: Therapeutic effects of low-intensity transcranial focused ultrasound stimulation on ischemic stroke in rats: An in vivo evaluation using electrical impedance tomography Article Snippet: They were then incubated overnight at 4°C with rabbit anti-brain-derived neurotrophic factor (BDNF; 1:2000, Abcam, Cambridge, UK, Cat# ab108319, RRID: AB_10862052) and rabbit anti-β-actin (1:1000, Proteintech, Chicago, IL, USA, Cat# 20536-1-AP, RRID: AB_10700003). .. After washing with Article Title: Clinical characteristics and spatial transcriptome analysis of non-small cell lung cancers exhibiting early alectinib resistance: a retrospective OLCSG study Article Snippet: .. The slides were incubated in pure water containing Article Title: Tet2 deficiency–induced expansion of monocyte-derived macrophages promotes liver fibrosis Article Snippet: .. Proteins in SDS-PAGE gel was transferred to nitrocellulose membrane for 1.5 h. The targeted strips were cut according to molecular weight and incubated by 5% skim milk at room temperature for 1 h. After washing thrice with Article Title: Ginsenoside Rb1 Maintains Tunneling Nanotubes between Astrocytes and Neurons To Protect Mice from Cerebral ischemia/reperfusion Injury. Article Snippet: 1 Department of Neurosurgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo 315040, Zhejiang, China 2 Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, Nanchang 330008, Jiangxi, China 3 Emergency and Trauma Center, The Affiliated Lihuili Hospital of Ningbo University, Ningbo 315040, Zhejiang, China 4 Department of Cerebrovascular Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China 5 Department of Geriatrics, Yixing Traditional Chinese Medicine Hospital, No. 128, Yangquan East Road, Yicheng Sub-district, Yixing 214200, Jiangsu, China Abstract Ginsenoside Rb1 (GRb1), the major bioactive component of ginseng, exhibits multiple therapeutic effects.. However, its neuroprotective role in cerebral ischemia/reperfusion (I/R) injury remains unclear.. The neuroprotective effects of GRb1 were investigated using a mouse middle cerebral artery occlusion (MCAO) model and in vitro oxygen-glucose deprivation/ reoxygenation (OGD/R) models. Control:Article Title: Tet2 deficiency–induced expansion of monocyte-derived macrophages promotes liver fibrosis Article Snippet: .. For each RIP, 50 μl lysate was diluted in 450 μl dilution buffer (0.01% SDS, 1.1% Triton X-100, 167 mM NaCl, and 16.7 mM Membrane:Article Title: Intelectin-2 is a broad-spectrum antimicrobial lectin Article Snippet: To verify protein expression, expression media was combined with 6x Laemmli buffer containing dithiothreitol, boiled at 95 °C for 10 min, and run on 4–15% TGX gel (BioRad). .. Proteins were transferred to PVDF membrane, blocked in 5% Article Title: Intelectin-2 is a broad-spectrum antimicrobial lectin. Article Snippet: To verify protein expression, expression media was combined with 6x Laemmli buffer containing dithiothreitol, boiled at 95 °C for 10min, and run on 4–15% TGX gel (BioRad). .. Proteins were transferred to PVDF membrane, blocked in 5% Article Title: Tet2 deficiency–induced expansion of monocyte-derived macrophages promotes liver fibrosis Article Snippet: .. Proteins in SDS-PAGE gel was transferred to nitrocellulose membrane for 1.5 h. The targeted strips were cut according to molecular weight and incubated by 5% skim milk at room temperature for 1 h. After washing thrice with Saline:Article Title: Intelectin-2 is a broad-spectrum antimicrobial lectin Article Snippet: To verify protein expression, expression media was combined with 6x Laemmli buffer containing dithiothreitol, boiled at 95 °C for 10 min, and run on 4–15% TGX gel (BioRad). .. Proteins were transferred to PVDF membrane, blocked in 5% Article Title: Therapeutic effects of low-intensity transcranial focused ultrasound stimulation on ischemic stroke in rats: An in vivo evaluation using electrical impedance tomography Article Snippet: They were then incubated overnight at 4°C with rabbit anti-brain-derived neurotrophic factor (BDNF; 1:2000, Abcam, Cambridge, UK, Cat# ab108319, RRID: AB_10862052) and rabbit anti-β-actin (1:1000, Proteintech, Chicago, IL, USA, Cat# 20536-1-AP, RRID: AB_10700003). .. After washing with Article Title: Intelectin-2 is a broad-spectrum antimicrobial lectin. Article Snippet: To verify protein expression, expression media was combined with 6x Laemmli buffer containing dithiothreitol, boiled at 95 °C for 10min, and run on 4–15% TGX gel (BioRad). .. Proteins were transferred to PVDF membrane, blocked in 5% Article Title: Tet2 deficiency–induced expansion of monocyte-derived macrophages promotes liver fibrosis Article Snippet: .. Proteins in SDS-PAGE gel was transferred to nitrocellulose membrane for 1.5 h. The targeted strips were cut according to molecular weight and incubated by 5% skim milk at room temperature for 1 h. After washing thrice with Article Title: Ginsenoside Rb1 Maintains Tunneling Nanotubes between Astrocytes and Neurons To Protect Mice from Cerebral ischemia/reperfusion Injury. Article Snippet: 1 Department of Neurosurgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo 315040, Zhejiang, China 2 Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, Nanchang 330008, Jiangxi, China 3 Emergency and Trauma Center, The Affiliated Lihuili Hospital of Ningbo University, Ningbo 315040, Zhejiang, China 4 Department of Cerebrovascular Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China 5 Department of Geriatrics, Yixing Traditional Chinese Medicine Hospital, No. 128, Yangquan East Road, Yicheng Sub-district, Yixing 214200, Jiangsu, China Abstract Ginsenoside Rb1 (GRb1), the major bioactive component of ginseng, exhibits multiple therapeutic effects.. However, its neuroprotective role in cerebral ischemia/reperfusion (I/R) injury remains unclear.. The neuroprotective effects of GRb1 were investigated using a mouse middle cerebral artery occlusion (MCAO) model and in vitro oxygen-glucose deprivation/ reoxygenation (OGD/R) models. Expressing:Article Title: Intelectin-2 is a broad-spectrum antimicrobial lectin Article Snippet: To verify protein expression, expression media was combined with 6x Laemmli buffer containing dithiothreitol, boiled at 95 °C for 10 min, and run on 4–15% TGX gel (BioRad). .. Proteins were transferred to PVDF membrane, blocked in 5% Article Title: Intelectin-2 is a broad-spectrum antimicrobial lectin. Article Snippet: To verify protein expression, expression media was combined with 6x Laemmli buffer containing dithiothreitol, boiled at 95 °C for 10min, and run on 4–15% TGX gel (BioRad). .. Proteins were transferred to PVDF membrane, blocked in 5% Immunoprecipitation:Article Title: UBR5 regulates the progression of colorectal cancer cells through Snail-induced epithelial–mesenchymal transition Article Snippet: The membranes were probed with primary antibodies, including Flag (Proteintech, Wuhan, China, 66008-4-Ig), Myc (Proteintech, 60003-2-Ig), UBR5 (Proteintech, 66937-1-Ig), Snail (Santa Cruz Biotechnology, Oregon, USA, 166476), phosphorylated Snail (Biodragon, BD-PP0568), Slug (Santa Cruz Biotechnology, 271977), E-cadherin (Proteintech, 20874-1-AP), N-cadherin (BD Transduction Laboratories, Franklin Lakes, USA, 610920), GSK3β (Proteintech, 82061-1-RR), pGSK3β (Proteintech, 67558-1-Ig), green fluorescent protein (GFP; Proteintech, 66002-1-Ig), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Bioss, Woburn, USA, 0978M). .. For the immunoprecipitation assay, HEK293T cells were co-transfected with the indicated plasmids and treated with 10 μM proteasome inhibitor MG132 (Sigma–Aldrich, St. Louis, USA, C2211) for 6 h, cells were lysed, and the supernatants were incubated with Binding Assay:Article Title: UBR5 regulates the progression of colorectal cancer cells through Snail-induced epithelial–mesenchymal transition Article Snippet: The membranes were probed with primary antibodies, including Flag (Proteintech, Wuhan, China, 66008-4-Ig), Myc (Proteintech, 60003-2-Ig), UBR5 (Proteintech, 66937-1-Ig), Snail (Santa Cruz Biotechnology, Oregon, USA, 166476), phosphorylated Snail (Biodragon, BD-PP0568), Slug (Santa Cruz Biotechnology, 271977), E-cadherin (Proteintech, 20874-1-AP), N-cadherin (BD Transduction Laboratories, Franklin Lakes, USA, 610920), GSK3β (Proteintech, 82061-1-RR), pGSK3β (Proteintech, 67558-1-Ig), green fluorescent protein (GFP; Proteintech, 66002-1-Ig), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Bioss, Woburn, USA, 0978M). .. For the immunoprecipitation assay, HEK293T cells were co-transfected with the indicated plasmids and treated with 10 μM proteasome inhibitor MG132 (Sigma–Aldrich, St. Louis, USA, C2211) for 6 h, cells were lysed, and the supernatants were incubated with SDS Page:Article Title: Tet2 deficiency–induced expansion of monocyte-derived macrophages promotes liver fibrosis Article Snippet: .. Proteins in SDS-PAGE gel was transferred to nitrocellulose membrane for 1.5 h. The targeted strips were cut according to molecular weight and incubated by 5% skim milk at room temperature for 1 h. After washing thrice with Molecular Weight:Article Title: Tet2 deficiency–induced expansion of monocyte-derived macrophages promotes liver fibrosis Article Snippet: .. Proteins in SDS-PAGE gel was transferred to nitrocellulose membrane for 1.5 h. The targeted strips were cut according to molecular weight and incubated by 5% skim milk at room temperature for 1 h. After washing thrice with Blocking Assay:Article Title: Ginsenoside Rb1 Maintains Tunneling Nanotubes between Astrocytes and Neurons To Protect Mice from Cerebral ischemia/reperfusion Injury. Article Snippet: 1 Department of Neurosurgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo 315040, Zhejiang, China 2 Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, Nanchang 330008, Jiangxi, China 3 Emergency and Trauma Center, The Affiliated Lihuili Hospital of Ningbo University, Ningbo 315040, Zhejiang, China 4 Department of Cerebrovascular Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China 5 Department of Geriatrics, Yixing Traditional Chinese Medicine Hospital, No. 128, Yangquan East Road, Yicheng Sub-district, Yixing 214200, Jiangsu, China Abstract Ginsenoside Rb1 (GRb1), the major bioactive component of ginseng, exhibits multiple therapeutic effects.. However, its neuroprotective role in cerebral ischemia/reperfusion (I/R) injury remains unclear.. The neuroprotective effects of GRb1 were investigated using a mouse middle cerebral artery occlusion (MCAO) model and in vitro oxygen-glucose deprivation/ reoxygenation (OGD/R) models. |