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northernmax high stringency wash buffer (Thermo Fisher)

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Thermo Fisher northernmax high stringency wash buffer
Northernmax High Stringency Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+stringency+buffer/pmc09638912-70-15-20
Average 90 stars, based on 1 article reviews

northernmax high stringency wash buffer - by Bioz Stars, 2026-10

90/100 stars

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Article Title: OASIS/CREB3L1 Induces Expression of Genes Involved in Extracellular Matrix Production But Not Classical Endoplasmic Reticulum Stress Response Genes in Pancreatic ?-Cells
Article Snippet: The detection probe (25 p m ) was added to hybridization buffer (same buffer used in prehybridization) and incubated at 42 C for 16–18 h. After hybridization, the blot was subjected to washings (23 C/5min, 42 C/15 min (times 2) with NorthernMax low-stringency wash buffer (Ambion).

Article Title: Generation of an 870 kb deletion encompassing the Skt/Etl4 locus by combination of inter- and intra-chromosomal recombination
Article Snippet: Unspecific probe was removed by washing with NorthernMax® Low Stringency Wash buffer (Ambion) twice for five minutes and once with NorthernMax® High Stringency Wash buffer (Ambion) for 15 min at 42 °C.

Article Title: Evaluating Posttranscriptional Regulation of Cytokine Genes
Article Snippet: NorthernMax® Low Stringency Wash Buffer (Ambion).

Article Title: Evaluating Posttranscriptional Regulation of Cytokine Genes
Article Snippet: NorthernMax® High Stringency Wash Buffer (Ambion).

Membrane:

Article Title: Expansion of the genetic code through reassignment of redundant sense codons using fully modified tRNA
Article Snippet: .. The following morning, the membrane was washed with 2 × 10 ml of pre-heated (42°C) NorthernMax High Stringency Wash Buffer (Invitrogen), each wash rocking at 42°C for 15 min. Staining buffer was prepared in the dark by mixing 100 μl of 10% sodium dodecylsulfate (SDS), 50 μl of 20% Tween-20 and 10 μl of IRDye 800 CW Streptavidin (Licor, 926-32230) and diluting to 10 ml in 1× phosphate-buffered saline (PBS) buffer. ..

Staining:

Saline:

Hybridization:

Article Title: An optimized isolation and labeling platform for accurate microRNA expression profiling
Article Snippet: For Northern analyses, RNA samples were resolved on denaturing 15% polyacrylamide gels and electroblotted on BrightStar-Plus Nylon membranes (Ambion). .. Membranes were blocked in UltraHyb-oligo Hybridization Buffer (Ambion) for at least 1 h at 65°C, hybridized overnight at 42°C with the appropriate probe, and washed three times with NorthernMax Low Stringency Wash Buffer (Ambion) for 5 min at room temperature followed by 15 min at 42°C. .. Results were quantified on a PhosphorImager Storm 860 (Amersham Bioscience).