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high stringency wash buffer  (Zymo Research)


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    Structured Review

    Zymo Research high stringency wash buffer
    High Stringency Wash Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/M-Wash+Buffer/pm40185099-433-38-74
    Average 94 stars, based on 59 article reviews
    high stringency wash buffer - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Purification:

    Article Title: An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation.
    Article Snippet: 50 mL streptavidin C1 magnetic beads (Thermo Fisher Scientific) were added and reactions were incubated at 37 C for 45 minutes to capture the complexes. .. The beads were washed three times with 250 mL of Low Stringency Wash Buffer (13 SSPE, 0.1% SDS, 1% Molecular Cell 85, 1–18.e1–e10, April 17, 2025 e9 NP-40, 4 M urea) and three times with 250 mL of High Stringency Wash Buffer (0.13 SSPE, 0.1% SDS, 1% NP-40, 4 M urea), each wash for 5 minutes at 60 C. RNA was eluted using RNase H, purified using TRIzol and RNA Clean & Concentrator-5 columns (Zymo Research), and then subjected to qRT-PCR as described above. ..

    Quantitative RT-PCR:

    Article Title: An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation.
    Article Snippet: 50 mL streptavidin C1 magnetic beads (Thermo Fisher Scientific) were added and reactions were incubated at 37 C for 45 minutes to capture the complexes. .. The beads were washed three times with 250 mL of Low Stringency Wash Buffer (13 SSPE, 0.1% SDS, 1% Molecular Cell 85, 1–18.e1–e10, April 17, 2025 e9 NP-40, 4 M urea) and three times with 250 mL of High Stringency Wash Buffer (0.13 SSPE, 0.1% SDS, 1% NP-40, 4 M urea), each wash for 5 minutes at 60 C. RNA was eluted using RNase H, purified using TRIzol and RNA Clean & Concentrator-5 columns (Zymo Research), and then subjected to qRT-PCR as described above. ..

    Centrifugation:

    Article Title: Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA
    Article Snippet: The DNA bound column was washed once with 100 μL Zymo M-Washing buffer (Zymo Research), then 200 μL of M-Desulphonation buffer (Zymo Research) was added and allowed to sit on the column for 15 minutes at room temperature. .. After centrifugation, a total of four washing steps were completed using 200 μL of Zymo M-Wash buffer each time. ..

    Article Title: Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA
    Article Snippet: The DNA bound column was washed once with 100 μL Zymo M-Washing buffer (Zymo Research), then 200 μL of M-Desulphonation buffer (Zymo Research) was added and allowed to sit on the column for 15 minutes at room temperature. .. After centrifugation, a total of four washing steps were completed using 200 μL of Zymo M-Wash buffer each time. ..



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