Review



high stringency wash buffer  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Santa Cruz Biotechnology high stringency wash buffer
    High Stringency Wash Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 11767 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/Triton+X-100/pm37169843-399-43-24
    Average 96 stars, based on 11767 article reviews
    high stringency wash buffer - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Biomimetic scaffolds mimic human subretinal debris for preclinical testing of prosthetic vision
    Article Snippet: .. Samples were incubated for 48 h at room temperature with mouse anti-PKCα antibody (Santa Cruz Biotechnology, sc-8393) diluted in PBS containing 5% BSA and 0.5% Triton X-100. .. After washing with PBS containing 0.1% Tween-20, samples were incubated for 48 h at room temperature with Cy3-conjugated donkey anti-mouse secondary antibody (Jackson ImmunoResearch, 715-165-150).

    Article Title: Attractin-like protein 1 is an essential partner of MC4R to regulate body weight
    Article Snippet: Samples were embedded in Tissue-Tek OCT compound (Sakura Finetechical Co., Ltd., Tokyo) and sectioned at 35 μm thickness using a microtome (Leica Biosystems, Buffalo Grove, IL, USA). .. Free-floating sections were washed in PBS two times and incubated with blocking buffer containing 5% normal goat serum in PBS with 0.5% Triton X-100 for 1 h followed by rabbit polyclonal anti-cFos antibody (sc52, Santa Cruz Biotechnology, TX, USA) in blocking solution (1:1000) overnight at 4 °C. .. After washing with PBS containing 0.1% Tween-20 (PBST), the sections were incubated in 1:500 Alexa Fluor 594 goat anti-rabbit IgG (R37117, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature.

    Article Title: Magnolol-encapsulated PLGA nanoparticles attenuate inflammatory responses and oxidative stress in human peritoneal macrophages isolated from patients with cirrhosis
    Article Snippet: .. Briefly, after 4 h and 24 h of incubation, the cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 2% bovine serum albumin and labeled with 1:100 dilution of LAMP1 antibody (H4A3, Santa Cruz Biotechnology, USA). .. After washing, anti-Mouse IgG secondary antibody conjugated with Alexa Fluor 488 was added to the cells according to the manufacturer’s instructions (Thermo Fisher Scientific, USA).

    Article Title: Beta‐Arrestin‐2 Binds to Nucleolar Organizing Regions With P2X7 Receptor Stimulation and During Cell Division
    Article Snippet: .. Cells, grown in chamber slides, were washed four times with PBS at room temperature, fixed with 2% (wt/vol) paraformaldehyde in PBS for 20 min and permeabilized with 0.1% (wt/vol) Triton X- 100 in PBS for 15 min. After incubation for 15 min in the blocking buffer (1% normal goat serum, 0.2% [vol/vol] Triton X- 100 in PBS), cells were incubated for 1 h at room temperature in 0.1% (wt/vol) BSA in PBS with the primary antibodies (Santa Cruz Biotechnology, Cat. Nos: Sc- 166 001, Sc- 55 486 and Sc- 13 125 for anti- fibrillarin, anti- nucleolin and anti- UBF, respectively) at a 1:50 dilution. .. After washing four times with 0.1% BSA in PBS, cells were incubated for 1 h at room temperature with Alexa Fluor 594- labeled anti- rabbit antibody (Jackson ImmunoResearch, Cat. No: 111–585- 045) at a 1:100 dilution.

    Article Title: YAP/TAZ-controlled ERK dynamics coordinate progenitor expansion and differentiation commitment
    Article Snippet: .. Primary antibodies were diluted in PBS containing 1% BSA and 0.3% Triton X-100 and incubated overnight at 4 °C: YAP (Cell Signaling Technology, D8H1X, cat. 14074, 1:1000), TAZ (Cell Signaling Technology, E8E9G, cat. 83669, 1:1000), PPARG (Santa Cruz Biotechnology, E-8, sc-7273, 1:1000), Pan-TEAD (Cell Signaling Technology, D3F7L, 1:1000), phospho-p44/42 MAPK (Thr202/Tyr204) (Cell Signaling Technology, 4370, 1:400), and total p44/42 MAPK (Cell Signaling Technology, 4696, 1:400). .. Cells were washed three times with PBS, incubated for 1.5 h with Alexa Fluor-conjugated anti-rabbit or anti-mouse secondary antibodies (Thermo Fisher Scientific, 1:1000) in PBS containing 1% BSA and 0.3% Triton X-100, then counterstained with DAPI for 30 min at room temperature and washed three times with PBS before imaging.

    Blocking Assay:

    Article Title: Attractin-like protein 1 is an essential partner of MC4R to regulate body weight
    Article Snippet: Samples were embedded in Tissue-Tek OCT compound (Sakura Finetechical Co., Ltd., Tokyo) and sectioned at 35 μm thickness using a microtome (Leica Biosystems, Buffalo Grove, IL, USA). .. Free-floating sections were washed in PBS two times and incubated with blocking buffer containing 5% normal goat serum in PBS with 0.5% Triton X-100 for 1 h followed by rabbit polyclonal anti-cFos antibody (sc52, Santa Cruz Biotechnology, TX, USA) in blocking solution (1:1000) overnight at 4 °C. .. After washing with PBS containing 0.1% Tween-20 (PBST), the sections were incubated in 1:500 Alexa Fluor 594 goat anti-rabbit IgG (R37117, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature.

    Article Title: Beta‐Arrestin‐2 Binds to Nucleolar Organizing Regions With P2X7 Receptor Stimulation and During Cell Division
    Article Snippet: .. Cells, grown in chamber slides, were washed four times with PBS at room temperature, fixed with 2% (wt/vol) paraformaldehyde in PBS for 20 min and permeabilized with 0.1% (wt/vol) Triton X- 100 in PBS for 15 min. After incubation for 15 min in the blocking buffer (1% normal goat serum, 0.2% [vol/vol] Triton X- 100 in PBS), cells were incubated for 1 h at room temperature in 0.1% (wt/vol) BSA in PBS with the primary antibodies (Santa Cruz Biotechnology, Cat. Nos: Sc- 166 001, Sc- 55 486 and Sc- 13 125 for anti- fibrillarin, anti- nucleolin and anti- UBF, respectively) at a 1:50 dilution. .. After washing four times with 0.1% BSA in PBS, cells were incubated for 1 h at room temperature with Alexa Fluor 594- labeled anti- rabbit antibody (Jackson ImmunoResearch, Cat. No: 111–585- 045) at a 1:100 dilution.

    Labeling:

    Article Title: Magnolol-encapsulated PLGA nanoparticles attenuate inflammatory responses and oxidative stress in human peritoneal macrophages isolated from patients with cirrhosis
    Article Snippet: .. Briefly, after 4 h and 24 h of incubation, the cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 2% bovine serum albumin and labeled with 1:100 dilution of LAMP1 antibody (H4A3, Santa Cruz Biotechnology, USA). .. After washing, anti-Mouse IgG secondary antibody conjugated with Alexa Fluor 488 was added to the cells according to the manufacturer’s instructions (Thermo Fisher Scientific, USA).

    Cell-Signaling:

    Article Title: YAP/TAZ-controlled ERK dynamics coordinate progenitor expansion and differentiation commitment
    Article Snippet: .. Primary antibodies were diluted in PBS containing 1% BSA and 0.3% Triton X-100 and incubated overnight at 4 °C: YAP (Cell Signaling Technology, D8H1X, cat. 14074, 1:1000), TAZ (Cell Signaling Technology, E8E9G, cat. 83669, 1:1000), PPARG (Santa Cruz Biotechnology, E-8, sc-7273, 1:1000), Pan-TEAD (Cell Signaling Technology, D3F7L, 1:1000), phospho-p44/42 MAPK (Thr202/Tyr204) (Cell Signaling Technology, 4370, 1:400), and total p44/42 MAPK (Cell Signaling Technology, 4696, 1:400). .. Cells were washed three times with PBS, incubated for 1.5 h with Alexa Fluor-conjugated anti-rabbit or anti-mouse secondary antibodies (Thermo Fisher Scientific, 1:1000) in PBS containing 1% BSA and 0.3% Triton X-100, then counterstained with DAPI for 30 min at room temperature and washed three times with PBS before imaging.



    Similar Products

    98
    Bio-Rad high stringency buffer
    High Stringency Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/Tris/pmc12354899-401-6-32
    Average 98 stars, based on 1 article reviews
    high stringency buffer - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    96
    Proteintech high stringency buffer
    High Stringency Buffer, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/PRDM10+Antibody/pmc12354899-401-6-38
    Average 96 stars, based on 1 article reviews
    high stringency buffer - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    90
    Active Motif high-stringency co-ip buffer
    High Stringency Co Ip Buffer, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/high+stringency+buffer+option/pm40490791-296-0-5
    Average 90 stars, based on 1 article reviews
    high-stringency co-ip buffer - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    94
    Zymo Research high stringency wash buffer
    High Stringency Wash Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/M-Wash+Buffer/pm40185099-433-38-74
    Average 94 stars, based on 1 article reviews
    high stringency wash buffer - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher high stringency buffer
    High Stringency Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/TRIS-HCL/bio_rxiv__2023__09__04__556039-493-14-35
    Average 99 stars, based on 1 article reviews
    high stringency buffer - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    96
    Santa Cruz Biotechnology high stringency wash buffer
    High Stringency Wash Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/Triton+X-100/pm37169843-399-43-24
    Average 96 stars, based on 1 article reviews
    high stringency wash buffer - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    90
    Thermo Fisher northern max low high stringency buffers
    Northern Max Low High Stringency Buffers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/pmc02529318-331-19-23
    Average 90 stars, based on 1 article reviews
    northern max low high stringency buffers - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher northernmax high stringency wash buffer
    Northernmax High Stringency Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/pmc09638912-70-15-20
    Average 90 stars, based on 1 article reviews
    northernmax high stringency wash buffer - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Active Motif high stringency wash buffer
    High Stringency Wash Buffer, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/high+stringency+buffer+option/pm34013328-81-7-6
    Average 90 stars, based on 1 article reviews
    high stringency wash buffer - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Active Motif high stringency ip buffer
    High Stringency Ip Buffer, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+stringency+buffer/high+stringency+buffer+option/pmc08860095-292-14-21
    Average 90 stars, based on 1 article reviews
    high stringency ip buffer - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results