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gradient function  (Bio-Rad)


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    Bio-Rad gradient function
    Gradient Function, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gradient+function/Dual+48%2F48+Fast+Reaction+Module/pmc12067301-234-3-5
    Average 94 stars, based on 44 article reviews
    gradient function - by Bioz Stars, 2026-09
    94/100 stars

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    other:

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    Recombinant:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Plasmid Preparation:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Software:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Polymerase Chain Reaction:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Article Title: Effective disposal and remediation of chemical agents with designer living biofilm materials in soil and water
    Article Snippet: .. A Bio-Rad S1000 Thermal Cycler with Dual 48/48 Fast Reaction Modules (Bio-Rad) was used to perform PCR, ligations, and restriction digests. .. Gel extractions were carried out with QIAquick Gel Extraction Kits (Qiagen).

    Sterility:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Blocking Assay:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Concentration Assay:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Modification:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Selection:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Marker:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..



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