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Promega goscript reverse transcriptase kit
Goscript Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+reverse+transcriptase/goscript+reverse+transcriptase+kit/pmc12269486-135-6-10
Average 90 stars, based on 1 article reviews
goscript reverse transcriptase kit - by Bioz Stars, 2026-09
90/100 stars

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Reverse Transcription:

Article Title: Generation of bovine iPSCs from fetal fibroblasts for in vitro myogenesis and cultured meat
Article Snippet: The Ribonucleic Acid (RNA) samples were analyzed regarding quantity and quality using a spectrophotometer (NanoDrop Lite Plus Spectrophotometer, cat. NDLPLUSULCA). .. Reverse transcription of the extracted RNA was performed using the commercial GoScriptTM Reverse Transcriptase (Promega, cat. A2801), according to the manufacturer’s instructions. ..

Article Title: Multiple-omics analyses of the Alviniconcha holobiont reveal multi-faceted adaptations to deep-sea hydrothermal vents
Article Snippet: According to the manufacturer’s instructions, total RNA was extracted using an Eastep ® Super Total RNA Extraction Kit (Promega, USA) and purified using the RNA purification and recovery Kit (Genstone, China). .. The first strand of cDNA from the gill RNA was synthesized using a GoScript TM Reverse Transcriptase kit (Promega, USA) according to the manufacturer’s instruction and used for PCR amplification (These primers were shown in Table S17 in Supplementary Table). .. Then, the PCR products were cloned into the pEASY-T3 vector (TransGen, China), and the clone plasmids were purified using the TIANprep Mini Plasmid Kit (TIANGEN, China) and sequenced using the Sanger method.

Article Title: Isolation and characterization of human cKIT positive amniotic fluid stem cells obtained from pregnancies with spina bifida.
Article Snippet: .. 1000ng of RNA from each sample was used for cDNA synthesis using GoScript Reverse Transcriptase Kit according to manufacturer’s instructions (Promega, Dubendorf, Switzerland). .. SYBR Green Master Mix (ThermoFisher Scientific, Basel, Switzerland) was used to perform qRT-PCR analysis.

Article Title: The gut microbiota mediates epoxy eicosanoid metabolism in the colon.
Article Snippet: Quantitative real-time reverse transcription PCR (qRT-PCR) Total RNA was isolated from the colon tissues using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized using the GoScript Reverse Transcriptase Kit (Promega). qRT-PCR was performed using the CFX Duet Real-Time PCR System (Bio-Rad Laboratories) with iTaq Universal SYBR Green Supermix (BioRad Laboratories). ..

Article Title: Treatment of a metabolic liver disease in mice with a transient prime editing approach.
Article Snippet: RNA was isolated from shock-frozen liver samples using the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. .. RNA was reverse transcribed to cDNA using random primers and GoScript Reverse Transcriptase kit (Promega). .. Reverse transcription quantitative PCR (RT–qPCR) was performed using Firepol qPCR Master Mix (Solis BioDyne) and analysed by 7900HT Fast Real-Time PCR System (Applied Biosystems).

Article Title: The gut microbiota mediates epoxy eicosanoid metabolism in the colon
Article Snippet: Total RNA was isolated from the colon tissues using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Complementary DNA was synthesized using the GoScript Reverse Transcriptase Kit (Promega). qRT–PCR was performed using the CFX Duet Real-Time PCR System (Bio-Rad Laboratories) with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). ..

Article Title: A tardigrade cytochrome P450 perturbs Drosophila melanogaster development
Article Snippet: .. TRIsure (Bioline), DNase I (Thermo Fisher Scientific), GoScript Reverse Transcriptase kit (Promega), SsoAdvanced Universal SYBR Green Supermix (Bio–Rad), CFX96 C1000 Touch System (Bio–Rad). ..

Article Title: HIV-1 binds dynein directly to hijack microtubule transport machinery.
Article Snippet: For qPCR, RNA was isolated and purified from cells using the NucleoSpin RNA extraction kit (Macherey- Nagel). .. Following RNA isolation, cDNA conversion was performed using the GoScript Reverse Transcriptase Kit (Promega). qPCR was performed using separate sets of primers amplifying various regions of each gene. ..

Synthesized:

Article Title: Multiple-omics analyses of the Alviniconcha holobiont reveal multi-faceted adaptations to deep-sea hydrothermal vents
Article Snippet: According to the manufacturer’s instructions, total RNA was extracted using an Eastep ® Super Total RNA Extraction Kit (Promega, USA) and purified using the RNA purification and recovery Kit (Genstone, China). .. The first strand of cDNA from the gill RNA was synthesized using a GoScript TM Reverse Transcriptase kit (Promega, USA) according to the manufacturer’s instruction and used for PCR amplification (These primers were shown in Table S17 in Supplementary Table). .. Then, the PCR products were cloned into the pEASY-T3 vector (TransGen, China), and the clone plasmids were purified using the TIANprep Mini Plasmid Kit (TIANGEN, China) and sequenced using the Sanger method.

Article Title: The gut microbiota mediates epoxy eicosanoid metabolism in the colon.
Article Snippet: Quantitative real-time reverse transcription PCR (qRT-PCR) Total RNA was isolated from the colon tissues using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized using the GoScript Reverse Transcriptase Kit (Promega). qRT-PCR was performed using the CFX Duet Real-Time PCR System (Bio-Rad Laboratories) with iTaq Universal SYBR Green Supermix (BioRad Laboratories). ..

Article Title: The gut microbiota mediates epoxy eicosanoid metabolism in the colon
Article Snippet: Total RNA was isolated from the colon tissues using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Complementary DNA was synthesized using the GoScript Reverse Transcriptase Kit (Promega). qRT–PCR was performed using the CFX Duet Real-Time PCR System (Bio-Rad Laboratories) with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). ..

Polymerase Chain Reaction:

Article Title: Multiple-omics analyses of the Alviniconcha holobiont reveal multi-faceted adaptations to deep-sea hydrothermal vents
Article Snippet: According to the manufacturer’s instructions, total RNA was extracted using an Eastep ® Super Total RNA Extraction Kit (Promega, USA) and purified using the RNA purification and recovery Kit (Genstone, China). .. The first strand of cDNA from the gill RNA was synthesized using a GoScript TM Reverse Transcriptase kit (Promega, USA) according to the manufacturer’s instruction and used for PCR amplification (These primers were shown in Table S17 in Supplementary Table). .. Then, the PCR products were cloned into the pEASY-T3 vector (TransGen, China), and the clone plasmids were purified using the TIANprep Mini Plasmid Kit (TIANGEN, China) and sequenced using the Sanger method.

Amplification:

Article Title: Multiple-omics analyses of the Alviniconcha holobiont reveal multi-faceted adaptations to deep-sea hydrothermal vents
Article Snippet: According to the manufacturer’s instructions, total RNA was extracted using an Eastep ® Super Total RNA Extraction Kit (Promega, USA) and purified using the RNA purification and recovery Kit (Genstone, China). .. The first strand of cDNA from the gill RNA was synthesized using a GoScript TM Reverse Transcriptase kit (Promega, USA) according to the manufacturer’s instruction and used for PCR amplification (These primers were shown in Table S17 in Supplementary Table). .. Then, the PCR products were cloned into the pEASY-T3 vector (TransGen, China), and the clone plasmids were purified using the TIANprep Mini Plasmid Kit (TIANGEN, China) and sequenced using the Sanger method.

cDNA Synthesis:

Article Title: Isolation and characterization of human cKIT positive amniotic fluid stem cells obtained from pregnancies with spina bifida.
Article Snippet: .. 1000ng of RNA from each sample was used for cDNA synthesis using GoScript Reverse Transcriptase Kit according to manufacturer’s instructions (Promega, Dubendorf, Switzerland). .. SYBR Green Master Mix (ThermoFisher Scientific, Basel, Switzerland) was used to perform qRT-PCR analysis.

Quantitative RT-PCR:

Article Title: The gut microbiota mediates epoxy eicosanoid metabolism in the colon.
Article Snippet: Quantitative real-time reverse transcription PCR (qRT-PCR) Total RNA was isolated from the colon tissues using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized using the GoScript Reverse Transcriptase Kit (Promega). qRT-PCR was performed using the CFX Duet Real-Time PCR System (Bio-Rad Laboratories) with iTaq Universal SYBR Green Supermix (BioRad Laboratories). ..

Real-time Polymerase Chain Reaction:

Article Title: The gut microbiota mediates epoxy eicosanoid metabolism in the colon.
Article Snippet: Quantitative real-time reverse transcription PCR (qRT-PCR) Total RNA was isolated from the colon tissues using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized using the GoScript Reverse Transcriptase Kit (Promega). qRT-PCR was performed using the CFX Duet Real-Time PCR System (Bio-Rad Laboratories) with iTaq Universal SYBR Green Supermix (BioRad Laboratories). ..

Article Title: The gut microbiota mediates epoxy eicosanoid metabolism in the colon
Article Snippet: Total RNA was isolated from the colon tissues using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Complementary DNA was synthesized using the GoScript Reverse Transcriptase Kit (Promega). qRT–PCR was performed using the CFX Duet Real-Time PCR System (Bio-Rad Laboratories) with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). ..

Article Title: HIV-1 binds dynein directly to hijack microtubule transport machinery.
Article Snippet: For qPCR, RNA was isolated and purified from cells using the NucleoSpin RNA extraction kit (Macherey- Nagel). .. Following RNA isolation, cDNA conversion was performed using the GoScript Reverse Transcriptase Kit (Promega). qPCR was performed using separate sets of primers amplifying various regions of each gene. ..

SYBR Green Assay:

Article Title: The gut microbiota mediates epoxy eicosanoid metabolism in the colon.
Article Snippet: Quantitative real-time reverse transcription PCR (qRT-PCR) Total RNA was isolated from the colon tissues using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized using the GoScript Reverse Transcriptase Kit (Promega). qRT-PCR was performed using the CFX Duet Real-Time PCR System (Bio-Rad Laboratories) with iTaq Universal SYBR Green Supermix (BioRad Laboratories). ..

Article Title: The gut microbiota mediates epoxy eicosanoid metabolism in the colon
Article Snippet: Total RNA was isolated from the colon tissues using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Complementary DNA was synthesized using the GoScript Reverse Transcriptase Kit (Promega). qRT–PCR was performed using the CFX Duet Real-Time PCR System (Bio-Rad Laboratories) with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). ..

Article Title: A tardigrade cytochrome P450 perturbs Drosophila melanogaster development
Article Snippet: .. TRIsure (Bioline), DNase I (Thermo Fisher Scientific), GoScript Reverse Transcriptase kit (Promega), SsoAdvanced Universal SYBR Green Supermix (Bio–Rad), CFX96 C1000 Touch System (Bio–Rad). ..

Isolation:

Article Title: HIV-1 binds dynein directly to hijack microtubule transport machinery.
Article Snippet: For qPCR, RNA was isolated and purified from cells using the NucleoSpin RNA extraction kit (Macherey- Nagel). .. Following RNA isolation, cDNA conversion was performed using the GoScript Reverse Transcriptase Kit (Promega). qPCR was performed using separate sets of primers amplifying various regions of each gene. ..



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