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fluoroshield tm with dapi  (Beyotime)


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    Structured Review

    Beyotime fluoroshield tm with dapi
    Fluoroshield Tm With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroshield+tm+dapi/DAPI/pmc11237085-260-63-67
    Average 99 stars, based on 33033 article reviews
    fluoroshield tm with dapi - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Zyxin protects against hypertensive nephropathy by restraining FAK/Skp2-CDK8/YAP signaling in tubular epithelial cells.
    Article Snippet: 1 Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Nephrology, Huadong Hospital, Fudan University, Yan’an Road No. 221, Shanghai 200040, China Abstract Background Tubulointerstitial fibrosis and inflammation are hallmark pathological features of hypertensive nephropathy (HN), yet the underlying molecular mechanisms remain elusive.. Zyxin, a focal adhesion protein, has been implicated in hypertension-related pathologies, but its specific role in HN-induced tubulointerstitial injury remains undefined.. Methods An HN murine model was established via a multi-hit DOCA/Ang II + uninephrectomy + high salt approach.

    Article Title: SERINC3 promotes oral squamous cell carcinoma progression and mitochondrial metabolic reprogramming involving c-MET/JNK/PGC-1α axis.
    Article Snippet: .. Cells were incubated with the working solution (100 nM) at 37°C for 20 min and fixed with 4% paraformaldehyde for 20 min. Then cells were washed again with PBS and treated with a fluorescence quenching agent containing DAPI (Beyotime, P0131, China) to protect the fluorescent signal. ..

    Staining:

    Article Title: Zyxin protects against hypertensive nephropathy by restraining FAK/Skp2-CDK8/YAP signaling in tubular epithelial cells.
    Article Snippet: 1 Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Nephrology, Huadong Hospital, Fudan University, Yan’an Road No. 221, Shanghai 200040, China Abstract Background Tubulointerstitial fibrosis and inflammation are hallmark pathological features of hypertensive nephropathy (HN), yet the underlying molecular mechanisms remain elusive.. Zyxin, a focal adhesion protein, has been implicated in hypertension-related pathologies, but its specific role in HN-induced tubulointerstitial injury remains undefined.. Methods An HN murine model was established via a multi-hit DOCA/Ang II + uninephrectomy + high salt approach.

    Article Title: Sex-Stratified Characterization of Behavioral, Hippocampal, and Microglial Androgen Responses to Adolescent High-Fat Diet Consumption in Mice.
    Article Snippet: .. Finally, the cell nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; C1027, 1:600; Beyotime, Shanghai, China). ..

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with DAPI (Beyotime, C1006) for 5 min, and images were captured using a confocal fluorescence microscope (Leica, Germany). ..

    Article Title: Platelet-derived microparticles induce CD8 + T cell senescence in chronic kidney disease via the transfer of microRNA-550a-5p.
    Article Snippet: .. Nuclei were stained with DAPI (Beyotime). .. Visualization was performed using a Zeiss LSM800 NLO confocal microscopy (Carl Zeiss).

    Glass:

    Article Title: HOXA3 Knockdown Impairs hESC-Derived Thymic Epithelial-like Cell Differentiation and CXCL12-Associated Early T-Lineage Support.
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, Biotechnology, Shanghai, China) for 10 min at room temperature in the dark, and coverslips were mounted onto glass slides using an anti-fade mounting medium (Beyotime, China). .. Images were acquired using a Zeiss 880 multiphoton laser scanning microscope (Zeiss, Oberkochen, Germany) or a Nikon single-photon confocal microscope (Nikon, Tokyo, Japan).

    Fluorescence:

    Article Title: SERINC3 promotes oral squamous cell carcinoma progression and mitochondrial metabolic reprogramming involving c-MET/JNK/PGC-1α axis.
    Article Snippet: .. Cells were incubated with the working solution (100 nM) at 37°C for 20 min and fixed with 4% paraformaldehyde for 20 min. Then cells were washed again with PBS and treated with a fluorescence quenching agent containing DAPI (Beyotime, P0131, China) to protect the fluorescent signal. ..

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with DAPI (Beyotime, C1006) for 5 min, and images were captured using a confocal fluorescence microscope (Leica, Germany). ..

    Confocal:

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with DAPI (Beyotime, C1006) for 5 min, and images were captured using a confocal fluorescence microscope (Leica, Germany). ..

    Microscopy:

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with DAPI (Beyotime, C1006) for 5 min, and images were captured using a confocal fluorescence microscope (Leica, Germany). ..

    Modification:

    Article Title: Multimodal Programming of Extracellular Vesicles via Nano-Primed SECCS-Based 3D Microenvironments for Coordinated Ferroptosis Suppression and Myogenic Regeneration in Diabetes.
    Article Snippet: Chronic diabetic glucolipotoxicity is associated with progressive skeletal muscle atrophy, bone loss, cellular senescence, and ferroptotic injury.. Because ferroptosis inhibition alonemay not restore compromisedmyogenic capacity, we developed a combined stem cell-conditioning strategy integrating fullerol priming with the stem cell screen-enrich-combine(-biomaterials) circulating system (SECCS).. This integrated three-dimensional culture platform uses porous β-TCP granules and intermittent medium circulation to generate BMSCs-derived extracellular vesicles (EVs).

    Saline:

    Article Title: Multimodal Programming of Extracellular Vesicles via Nano-Primed SECCS-Based 3D Microenvironments for Coordinated Ferroptosis Suppression and Myogenic Regeneration in Diabetes.
    Article Snippet: Chronic diabetic glucolipotoxicity is associated with progressive skeletal muscle atrophy, bone loss, cellular senescence, and ferroptotic injury.. Because ferroptosis inhibition alonemay not restore compromisedmyogenic capacity, we developed a combined stem cell-conditioning strategy integrating fullerol priming with the stem cell screen-enrich-combine(-biomaterials) circulating system (SECCS).. This integrated three-dimensional culture platform uses porous β-TCP granules and intermittent medium circulation to generate BMSCs-derived extracellular vesicles (EVs).



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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    Image Search Results


    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Article Snippet: The specimens were washed with PBS and mounted in DAPI anti-fade aqueous medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific).

    Techniques: Expressing

    E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Article Snippet: The specimens were washed with PBS and mounted in DAPI anti-fade aqueous medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific).

    Techniques: Expressing

    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Article Snippet: After the final washing step with PBS, the strips were transferred into a droplet of the anti-fade DAPI mounting medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific, Waltham, MA, USA) dripped onto the microscope slide and oriented on their side to visualize the villus axis [ ].

    Techniques: Expressing

    E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Article Snippet: After the final washing step with PBS, the strips were transferred into a droplet of the anti-fade DAPI mounting medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific, Waltham, MA, USA) dripped onto the microscope slide and oriented on their side to visualize the villus axis [ ].

    Techniques: Expressing