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filament module  (Oxford Instruments)


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    Structured Review

    Oxford Instruments filament module
    Filament Module, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44474 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/filament/Imaris/pm42225069-440-8-11
    Average 99 stars, based on 44474 article reviews
    filament module - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Software:

    Article Title: Morphological plasticity facilitates lung adaptation and dynamic immune remodeling in Mycobacterium abscessus infection
    Article Snippet: .. To quantify bacterial internalization versus extracellular adherence, z-stack images (step size: 0.3 μm) were acquired and subjected to 3D volumetric reconstruction using Imaris v9.5 software. ..

    Article Title: Chrysanthemum Morifolium– Derived Nanovesicles Reduce Lactate‐Associated Histone Lactylation and Modulate Microglia‐Mediated Neuroinflammation in Sepsis‐Associated Encephalopathy
    Article Snippet: An excitation wavelength of 488 nm and emission wavelength of 519 nm were employed to visualize the Iba‐1 protein. .. Images of microglial branch nodes (1024 × 1024 pixel) and total branch length were reconstructed and quantitatively analyzed using the “Filaments” module of the Imaris 10.1.0 software. ..

    Morphology:

    Article Title: Frontal cortical neuroinflammation and circulating biomarkers following cardiopulmonary bypass in sheep.
    Article Snippet: Images were acquired using the Nikon-Crest Spinning Disk Confocal microscope, at 40× magnification (objective Plan-Apochromat, 40×/1.25, silicone), with the systemrecommended 0.35 μm step size for a z-stack size of 20.1 μm. .. Microglial morphology was analysed in Imaris v10.2 (Oxford Instruments, Abingdon, UK) [30]. ..

    other:

    Article Title: Multiple motor proteins regulate TDP-43 anterograde axonal transport.
    Article Snippet: a—top Representative micrographs of particles movement, segmentation and tracking determined with Imaris spots tracking algorithms.



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    (A) Example STORM render images of MVI and Ku80 under non-treated, cisplatin treatment (65 µM 4 h) and combined Cisplatin+TIP (65 µM and 25 µM 4 h) in HeLa cells (scale bar 2 μm). The Ku80 staining denotes the nucleus region which was taken forward for cluster analysis. (B) Cluster analysis of Ku80 nuclear organisation. Individual data points correspond to the average value for a cell ROI (n = 17). ****p <0.0001 by one-way ANOVA and Tukey’s multiple comparisons test. (C) Representative colocalisation heatmap of MVI and Ku80 clusters following cisplatin treatment/ DoC values of 1 are perfectly colocalised and -1 are separated from each other. (D) Histogram of DoC scores for Ku80 colocalised with MVI and MVI colocalised with Ku80. (E) Illustration of the DNA oligonucleotide, labelled with Atto550 and AlexaFluor647, designed for an in vitro DSB. The proteins are presented in hypothetical arrangements to bridge the DNA oligonucleotides to generate a FRET signal. (F) Representation of ALEX smFRET, whereby when the two oligonucleotides diffuse into the confocal volume, the lasers alternate the excitation during the transit, allowing the determination of smFRET and the presence of both dyes. (G) Representation of photon bursts for Donor detector, Acceptor detector and the resulting FRET burst. (H) Plots of FRET efficiency vs stoichiometry for 20 pM DNA oligonucleotides in the presence of 500 nM Ku70/80, then 500 nM XRCC4 (I) and 500 nM MVI plus 1 µM <t>F-actin</t> (J). (K) The number of FRET bursts for each experimental condition stated. **p<0.01 ****p <0.0001 by one-way ANOVA and Tukey’s multiple comparisons test. Error bars represent SEM.
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    Image Search Results


    (A) Example STORM render images of MVI and Ku80 under non-treated, cisplatin treatment (65 µM 4 h) and combined Cisplatin+TIP (65 µM and 25 µM 4 h) in HeLa cells (scale bar 2 μm). The Ku80 staining denotes the nucleus region which was taken forward for cluster analysis. (B) Cluster analysis of Ku80 nuclear organisation. Individual data points correspond to the average value for a cell ROI (n = 17). ****p <0.0001 by one-way ANOVA and Tukey’s multiple comparisons test. (C) Representative colocalisation heatmap of MVI and Ku80 clusters following cisplatin treatment/ DoC values of 1 are perfectly colocalised and -1 are separated from each other. (D) Histogram of DoC scores for Ku80 colocalised with MVI and MVI colocalised with Ku80. (E) Illustration of the DNA oligonucleotide, labelled with Atto550 and AlexaFluor647, designed for an in vitro DSB. The proteins are presented in hypothetical arrangements to bridge the DNA oligonucleotides to generate a FRET signal. (F) Representation of ALEX smFRET, whereby when the two oligonucleotides diffuse into the confocal volume, the lasers alternate the excitation during the transit, allowing the determination of smFRET and the presence of both dyes. (G) Representation of photon bursts for Donor detector, Acceptor detector and the resulting FRET burst. (H) Plots of FRET efficiency vs stoichiometry for 20 pM DNA oligonucleotides in the presence of 500 nM Ku70/80, then 500 nM XRCC4 (I) and 500 nM MVI plus 1 µM F-actin (J). (K) The number of FRET bursts for each experimental condition stated. **p<0.01 ****p <0.0001 by one-way ANOVA and Tukey’s multiple comparisons test. Error bars represent SEM.

    Journal: bioRxiv

    Article Title: Nuclear Myosin VI stabilises Ku-associated DNA ends during nonhomologous end joining

    doi: 10.64898/2026.09.01.748478

    Figure Lengend Snippet: (A) Example STORM render images of MVI and Ku80 under non-treated, cisplatin treatment (65 µM 4 h) and combined Cisplatin+TIP (65 µM and 25 µM 4 h) in HeLa cells (scale bar 2 μm). The Ku80 staining denotes the nucleus region which was taken forward for cluster analysis. (B) Cluster analysis of Ku80 nuclear organisation. Individual data points correspond to the average value for a cell ROI (n = 17). ****p <0.0001 by one-way ANOVA and Tukey’s multiple comparisons test. (C) Representative colocalisation heatmap of MVI and Ku80 clusters following cisplatin treatment/ DoC values of 1 are perfectly colocalised and -1 are separated from each other. (D) Histogram of DoC scores for Ku80 colocalised with MVI and MVI colocalised with Ku80. (E) Illustration of the DNA oligonucleotide, labelled with Atto550 and AlexaFluor647, designed for an in vitro DSB. The proteins are presented in hypothetical arrangements to bridge the DNA oligonucleotides to generate a FRET signal. (F) Representation of ALEX smFRET, whereby when the two oligonucleotides diffuse into the confocal volume, the lasers alternate the excitation during the transit, allowing the determination of smFRET and the presence of both dyes. (G) Representation of photon bursts for Donor detector, Acceptor detector and the resulting FRET burst. (H) Plots of FRET efficiency vs stoichiometry for 20 pM DNA oligonucleotides in the presence of 500 nM Ku70/80, then 500 nM XRCC4 (I) and 500 nM MVI plus 1 µM F-actin (J). (K) The number of FRET bursts for each experimental condition stated. **p<0.01 ****p <0.0001 by one-way ANOVA and Tukey’s multiple comparisons test. Error bars represent SEM.

    Article Snippet: Filamentous Actin was purchased from Cytoskeleton Inc (AKF99).

    Techniques: Staining, In Vitro