Journal: Journal of neuropathology and experimental neurology

Article Title: Hereditary ferritinopathy: a novel mutation, its cellular pathology, and pathogenetic insights.

doi: 10.1093/jnen/64.4.280

Figure Lengend Snippet: FIGURE 9. Hyaline deposits. Pallidum. (A) Pleomorphic hyaline deposits, some containing nuclei, within the same area of GPe as in Figure 6A. (B) Prussian-blue reactivity. (C) PTAH reactivity. (D) Polyclonal anti-ferritin immunoreactivity. (E) Rare association of hyaline deposits with GFAP-immunoreactive astrocyte. (F) HO-2-immunoreactivity. Original magnifications: (A) Hematoxylin and eosin, 1503; (B) Perl’s stain, 2203; (C) 2203; (D) 2503; (E) 2203; (F) 2503.

Article Snippet: In order to identify the cell(s) of origin of the unique vacuolated nuclei, antibodies to S-100 (polyclonal, 1:20k, Lot #941; Innovation Foundation, Toronto, Ontario, Canada), GFAP, CD68 (monoclonal, 1:200, Lot #120201; DAKO), and carbonic anhydrase II (CAII) (polyclonal, 100-401-136/4383, 1:15k with retrieval; Rockland, Gilbertsville, PA) were applied to sections of basal ganglia and cerebellum, while antibodies to synaptophysin (monoclonal, 1:100 with retrieval, #M0363-UC/0403; Biogenex, San Ramon, CA), PGP 9.5 (polyclonal, 1:1,000 with retrieval, Lot #11830; Biogenesis, Handsown, NH), neurofilament protein 2F11 (monoclonal, 1:350, #M0762/117 DAKO) (101), and amyloid precursor protein (APP A4; monoclonal, 1:20K with heat retrieval, #23080547, Chemicon) were applied to the section of basal ganglia.

Techniques: Staining

Journal: Discover. Oncology

Article Title: Pyroptosis-related genes regulate proliferation and invasion of pancreatic cancer and serve as the prognostic signature for modeling patient survival.

doi: 10.1007/s12672-022-00495-0

Figure Lengend Snippet: Fig. 5 Expression and survival analysis of PRGs in PDAC. A The protein expression score of staining and intensity of NLRP1 and CASP4 pro- teins in all PDAC samples (HPA database). Protein expression score is based on immunohistochemical data manually scored with regard to staining intensity (negative, weak, moderate or strong) and fraction of stained cells (< 25%, 25–75% or > 75%). B Immunohistochemi- cal staining of NLRP1 and CASP4 proteins in PDAC. C Methylation levels of CASP4 at chr11:104838825–104841325 and chr11:104826922– 104829422 in PDAC and para-cancer tissues. D The OS survival curves of NLRP1 and CASP4 mRNA expression level in PDAC. E CASP4 and NLRP1 immunohistochemistry for patients from Zhongnan Hospital (×200)

Article Snippet: To test whether the expression of CASP4 or NLRP1 affects the sensitivity of pancreatic cancer cells to MEK inhibitors, Trametinib (MedChemExpress, USA) was added to siRNA-transfected PANC-1 and Aspc-1 cells.

Techniques: Expressing, Staining, Immunohistochemical staining, Methylation, Immunohistochemistry

Journal: Discover. Oncology

Article Title: Pyroptosis-related genes regulate proliferation and invasion of pancreatic cancer and serve as the prognostic signature for modeling patient survival.

doi: 10.1007/s12672-022-00495-0

Figure Lengend Snippet: Fig. 6 PRGs regulated the proliferation and invasion of pancreatic cancer cells in vitro. A, B After siRNA transfection, qPCR and western blot was used to detect RNA and protein expression levels of CASP4 and NLRP1 respectively in PANC-1 and Aspc-1 cells. C CCK8 assay was used to detect the proliferation of PANC-1 and Aspc-1 cells after transfection with siRNA. D Transwell was used to detect the change of cell inva- sion ability after transfection with siRNA (*P < 0.05, **P < 0.01, ***P < 0.001)

Article Snippet: To test whether the expression of CASP4 or NLRP1 affects the sensitivity of pancreatic cancer cells to MEK inhibitors, Trametinib (MedChemExpress, USA) was added to siRNA-transfected PANC-1 and Aspc-1 cells.

Techniques: In Vitro, Transfection, Western Blot, Expressing, CCK-8 Assay

Journal: Discover. Oncology

Article Title: Pyroptosis-related genes regulate proliferation and invasion of pancreatic cancer and serve as the prognostic signature for modeling patient survival.

doi: 10.1007/s12672-022-00495-0

Figure Lengend Snippet: Fig. 9 NLRP1 was associated with KRAS and P53 mutations potentially. A The expression levels of NLRP1 were different in KRAS mutation, P53 mutation and wild-type tumor tissues respectively. B Correlation curve between NLRP1, KRAS and P53 expression levels based on TCGA database. C GSEA analysis of DEGs in high NLRP1 expression group. D PANC-1 and Aspc-1 cells were tested for LDH release 24 h after siNLRP1 transfection. E Cleavage of GSDMD and p-ERK were monitored by immunoblot analysis 48 h after siNLRP1 transfection (*P < 0.05, ***P < 0.001)

Article Snippet: To test whether the expression of CASP4 or NLRP1 affects the sensitivity of pancreatic cancer cells to MEK inhibitors, Trametinib (MedChemExpress, USA) was added to siRNA-transfected PANC-1 and Aspc-1 cells.

Techniques: Expressing, Mutagenesis, Transfection, Western Blot

Journal: Discover. Oncology

Article Title: Pyroptosis-related genes regulate proliferation and invasion of pancreatic cancer and serve as the prognostic signature for modeling patient survival.

doi: 10.1007/s12672-022-00495-0

Figure Lengend Snippet: Fig. 10 Analysis of drug sensitivity associated with PEGs. A Correlation of CASP4 and NLRP1 expression with multidrug sensitivity in GDSC. Red is positive correlation, which means the higher the gene expression, the more sensitive to the drug, while the blue is the opposite. The Spearman correlation represent the gene expression correlates with the drug. The positive correlation means that the gene high expression is resistant to the drug, vise verse. B Survival rate of cells exposed to trametinib at the concentrations of 10 µM/L for 48 or 72 h (*P < 0.05, **P < 0.01, ***P < 0.001)

Article Snippet: To test whether the expression of CASP4 or NLRP1 affects the sensitivity of pancreatic cancer cells to MEK inhibitors, Trametinib (MedChemExpress, USA) was added to siRNA-transfected PANC-1 and Aspc-1 cells.

Techniques: Expressing, Gene Expression