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fastquant rt kit  (tiangen biotech co)


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    Structured Review

    tiangen biotech co fastquant rt kit
    Fastquant Rt Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 2861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fastquant+rt+kit/FastQuant+RT+Kit/pmc13680859-198-5-8
    Average 96 stars, based on 2861 article reviews
    fastquant rt kit - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Green Extraction and Molecular Docking-Guided Discovery of Asiaticoside as a Multi-Target Modulator of the SIRT1/HMGB1/p53-p21 Axis in H 2 O 2 -Induced Senescent Endothelial Cells.
    Article Snippet: Complementary DNA (cDNA) was synthesised from 1 μg of total RNA using the FastQuant RT Kit with gDNase (TIANGEN) to remove genomic DNA contamination.

    Synthesized:

    Article Title: Major capsid protein interacts with protein disulfide isomerase A3 to promote largemouth bass ranavirus adhesion and enhance viral infection.
    Article Snippet: Largemouth bass ranavirus (LMBV) is a DNA virus that threatens various aquatic animals, yet the mechanism by which it attaches to host cells to initiate infection remains poorly understood.. Here, we report that the major capsid protein (MCP) of LMBV interacts with host protein disulfide-isomerase A3 (PDIA3).. Subsequently, PDIA3 was also found to be localized on the host cell membrane, which may play a critical role in mediating virus adhesion and infection.

    Article Title: A Hierarchical OhrR – EmrR Regulatory Circuit Fine‐Tunes the Vfm Quorum‐ Sensing System and Virulence in Dickeya oryzae
    Article Snippet: Total RNA was extracted from bacterial cultures at OD 600 = 1.0 using the SV Total RNA Isolation System (Promega). .. cDNA was synthesized using the FastQuant RT Kit (Tiangen). .. qPCR was performed with SuperReal PreMix Colour SYBR Green (Tiangen) on a QuantStudio 6 Flex system.

    Article Title: Investigation of Histamine-Related Key Genes in the Progression from Sjögren's Syndrome to Keratoconjunctivitis Sicca
    Article Snippet: RNA concentration was measured using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Inc.). .. Genomic DNA (gDNA) was removed at 42◦C for 3 min, and firststrand cDNA was synthesized from 1 μg of total RNA at 42◦C for 15 min followed by 95◦C for 3 min using the KR106 Fast Quant RT Kit with gDNA Eraser (cat. no. KR106-03; Tiangen Biotech Co., Ltd.). .. RTqPCR was subsequently performed using the QuantiNova SYBR Green PCR Kit (cat. no. 208054; Qiagen GmbH) according to the manufacturer’s protocol.

    Article Title: Aligned-to-random bilayered collagen nerve conduit enhances pain relief and nerve repair following surgical decompression in diabetic entrapment neuropathy
    Article Snippet: Diabetic entrapment neuropathy (DEN) is a clinically important subtype of diabetic peripheral neuropathy, characterized by neuropathic pain caused by metabolic dysregulation and chronic nerve compression.. While surgical decompression can relieve focal entrapment, its effectiveness in pain relief and nerve repair is often compromised by an adverse post-decompression microenvironment, including persistent inflammation and tissue adhesion.. Although various biomaterial-based strategies for peripheral nerve repair have been widely explored, few have been specifically designed to address the pathological and mechanical demands of DEN.

    Article Title: In vitro anti-fungal mechanism of azelaic acid against Botrytis cinerea and its efficacy in controlling gray mold on blueberry fruit
    Article Snippet: RNA was extracted from the mycelial samples of B. cinerea using the Triquick reagent (Solarbio, Beijing, China) following the procedures provided by the manufacturer. .. First-strand cDNA was synthesized with the utilization of the FastQuant RT Kit (Tiangen, Beijing, China). .. Gene transcription levels were assessed employing the CFX96 Touch RealTime PCR Detection System (Bio-Rad, USA) in accordance with the protocol provided with the SuperReal PreMix Plus (SYBR Green) kit (Tiangen, Beijing, China).

    Reverse Transcription:

    Article Title: An investigation of the dual mechanism of uORF-mediated regulation of GGP1 expression and the effects on drought stress in cotton
    Article Snippet: For glasshouse experiments, total RNA was extracted from the second true leaf of the cotton material with 3 biological replicates using Trizol reagent (Invitrogen, United States). .. After DNase I (Promega, United States) treatment, total RNA (5 μg for each sample) was reverse transcribed using a FastQuant RT Kit (Tiangen Biotech Co. Ltd., China) according to the manufacturer's protocol. .. Real-time PCR analysis was carried out using the Bio-Rad CFX ConnectTM Real-Time PCR Detection System (Hercules, United States) with SYBR Green SuperReal PreMix Plus (Tiangen Biotech Co. Ltd., China).

    Article Title: Inhibition of glycogen synthase 1 ameliorates imiquimod-induced psoriasis by suppressing JAK2-STAT3 signaling
    Article Snippet: .. Equal amounts of RNA were reverse transcribed into complementary DNA (cDNA) using the FastQuant RT Kit (Tiangen) following the manufacturer’s protocol. .. Quantitative real-time PCR was performed on an ABI QuantStudio 6 system using SYBR Green PCR Master Mix (TOYOBO) with gene-specific primers (sequences listed in Table S1).



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