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microarray-based gene expression analysis protocol  (Arraystar inc)

 
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    Arraystar inc microarray-based gene expression analysis protocol
    Microarray Based Gene Expression Analysis Protocol, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+microarray+analysis/gene+expression+microarray+analysis/pm39096478-76-13-13
    Average 90 stars, based on 1 article reviews
    microarray-based gene expression analysis protocol - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Infection:

    Article Title: HCV-Associated Exosomes Upregulate RUNXOR and RUNX1 Expressions to Promote MDSC Expansion and Suppressive Functions through STAT3–miR124 Axis
    Article Snippet: .. To determine whether lncRNAs play a role in MDSC expansion during HCV infection, we analyzed the transcripts of lncRNAs and messenger RNAs (mRNAs) in MDSCs isolated from HCV-infected individuals and HS using the Arraystar gene expression array. ..

    Isolation:

    Article Title: HCV-Associated Exosomes Upregulate RUNXOR and RUNX1 Expressions to Promote MDSC Expansion and Suppressive Functions through STAT3–miR124 Axis
    Article Snippet: .. To determine whether lncRNAs play a role in MDSC expansion during HCV infection, we analyzed the transcripts of lncRNAs and messenger RNAs (mRNAs) in MDSCs isolated from HCV-infected individuals and HS using the Arraystar gene expression array. ..

    Article Title: Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway
    Article Snippet: .. CD4 T cells were isolated from HS and cultured with 5 μM KML001 or DPBS control for 48 h. Approximately 6 × 10 6 cells per group were sent to Arraystar Inc. (Rockville, MD) for gene expression analysis. ..

    Gene Expression:

    Article Title: HCV-Associated Exosomes Upregulate RUNXOR and RUNX1 Expressions to Promote MDSC Expansion and Suppressive Functions through STAT3–miR124 Axis
    Article Snippet: .. To determine whether lncRNAs play a role in MDSC expansion during HCV infection, we analyzed the transcripts of lncRNAs and messenger RNAs (mRNAs) in MDSCs isolated from HCV-infected individuals and HS using the Arraystar gene expression array. ..

    Article Title: Histone acetyltransferase 7 (KAT7)-dependent intragenic histone acetylation regulates endothelial cell gene regulation
    Article Snippet: .. Gene expression microarray analysis Total RNA of HUVEC samples treated with control siRNA or KAT7 siRNAs ( n = 4) were processed by Arraystar Inc. for gene expression microarray analysis. .. The microarray used for sample hybridization is the human LncRNA microarray V3.0, a custom 8 × 60K Agilent array containing 58,944 probes that detect 26,109 mRNA transcripts and 30,586 long non-coding RNA transcripts.

    Article Title: Mitochondrial Functions Are Compromised in CD4 T Cells From ART-Controlled PLHIV
    Article Snippet: .. The gene expression analysis was performed by Arraystar Inc (Rockville, MD) and the heat map was generated using an online heatmapper software following the Average linkage clustering and Euclidean distance measurement methods ( ). ..

    Article Title: Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway
    Article Snippet: .. CD4 T cells were isolated from HS and cultured with 5 μM KML001 or DPBS control for 48 h. Approximately 6 × 10 6 cells per group were sent to Arraystar Inc. (Rockville, MD) for gene expression analysis. ..

    Article Title: Telomere and ATM Dynamics in CD4 T-Cell Depletion in Active and Virus-Suppressed HIV Infections
    Article Snippet: .. Gene expression analysis in HIV-infected and 420 uninfected SupT1 cells and primary CD4 T cells was performed by Arraystar (Arraystar Inc, Rockville, MD). ..

    Microarray:

    Article Title: Histone acetyltransferase 7 (KAT7)-dependent intragenic histone acetylation regulates endothelial cell gene regulation
    Article Snippet: .. Gene expression microarray analysis Total RNA of HUVEC samples treated with control siRNA or KAT7 siRNAs ( n = 4) were processed by Arraystar Inc. for gene expression microarray analysis. .. The microarray used for sample hybridization is the human LncRNA microarray V3.0, a custom 8 × 60K Agilent array containing 58,944 probes that detect 26,109 mRNA transcripts and 30,586 long non-coding RNA transcripts.

    Control:

    Article Title: Histone acetyltransferase 7 (KAT7)-dependent intragenic histone acetylation regulates endothelial cell gene regulation
    Article Snippet: .. Gene expression microarray analysis Total RNA of HUVEC samples treated with control siRNA or KAT7 siRNAs ( n = 4) were processed by Arraystar Inc. for gene expression microarray analysis. .. The microarray used for sample hybridization is the human LncRNA microarray V3.0, a custom 8 × 60K Agilent array containing 58,944 probes that detect 26,109 mRNA transcripts and 30,586 long non-coding RNA transcripts.

    Article Title: Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway
    Article Snippet: .. CD4 T cells were isolated from HS and cultured with 5 μM KML001 or DPBS control for 48 h. Approximately 6 × 10 6 cells per group were sent to Arraystar Inc. (Rockville, MD) for gene expression analysis. ..

    other:

    Article Title: MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis
    Article Snippet: Total RNA was extracted from KAS-6/1 and U266 cells and sent to Aksomics (Shanghai, China) for RNA microarray analysis using the ArrayStar Gene Expression Array (Human, 4 × 44 K, Rockville, MD, USA).

    Article Title: Synergistic Effects of Glutamine Deprivation and Metformin in Acute Myeloid Leukemia.
    Article Snippet: [Abstract] Objective: The metabolic reprogramming of acute myeloid leukemia (AML) cells is a compensatory adaptation to meet energy requirements for rapid proliferation.. This study aimed to examine the synergistic effects of glutamine deprivation and metformin exposure on AML cells.. Methods: SKM-1 cells (an AML cell line) were subjected to glutamine deprivation and/or treatment with metformin or bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl) ethyl sulfide (BPTES, a glutaminase inhibitor) or cytarabine.

    Article Title: Transcriptome analysis of life stages of the house cricket, Acheta domesticus, to improve insect crop production.
    Article Snippet: Gene expression in each life stage was analyzed by ArrayStar (DNAStar).

    Generated:

    Article Title: Mitochondrial Functions Are Compromised in CD4 T Cells From ART-Controlled PLHIV
    Article Snippet: .. The gene expression analysis was performed by Arraystar Inc (Rockville, MD) and the heat map was generated using an online heatmapper software following the Average linkage clustering and Euclidean distance measurement methods ( ). ..

    Software:

    Article Title: Mitochondrial Functions Are Compromised in CD4 T Cells From ART-Controlled PLHIV
    Article Snippet: .. The gene expression analysis was performed by Arraystar Inc (Rockville, MD) and the heat map was generated using an online heatmapper software following the Average linkage clustering and Euclidean distance measurement methods ( ). ..

    Cell Culture:

    Article Title: Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway
    Article Snippet: .. CD4 T cells were isolated from HS and cultured with 5 μM KML001 or DPBS control for 48 h. Approximately 6 × 10 6 cells per group were sent to Arraystar Inc. (Rockville, MD) for gene expression analysis. ..



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    Agilent technologies microarray-based gene expression analysis guide
    NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by <t>microarray</t> analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.
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    Image Search Results


    NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by microarray analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.

    Journal: Journal of Experimental Botany

    Article Title: NAC61 regulates late- and post-ripening osmotic, oxidative, and biotic stress responses in grapevine

    doi: 10.1093/jxb/erad507

    Figure Lengend Snippet: NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by microarray analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.

    Article Snippet: The cDNA synthesis, labelling, hybridization, and washing steps were performed according to the Agilent Microarray-Based Gene Expression Analysis Guide ( https://www.agilent.com/cs/library/usermanuals/Public/G4140-90040_GeneExpression_OneColor_6.9.pdf ).

    Techniques: Expressing, Microarray