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5 ethynyl 2 deoxyuridine assay  (Yesen Biotech)

 
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    Yesen Biotech 5 ethynyl 2 deoxyuridine assay
    5 Ethynyl 2 Deoxyuridine Assay, supplied by Yesen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethynyl/2+5+assay+deoxyuridine+ethynyl/pm42320249-68-13-15
    Average 86 stars, based on 1 article reviews
    5 ethynyl 2 deoxyuridine assay - by Bioz Stars, 2026-09
    86/100 stars

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    <t>Proliferation</t> of minced cartilage and isolated cells on day 28. A biopsy from a chondral allograft, minced cartilage minced manually or arthroscopically and isolated chondrons and chondrocytes were cultured in fibrin gel and proliferation was determined on day 28. (a) Graph showing the percentage of proliferated cells in minced cartilage and isolated cells on day 28. Each point represents a donor with a minimum of one replicate per donor. Each donor is represented by a different symbol. Standard deviations are shown. A GEE analysis with a Bonferroni post-hoc correction for multiple testing between all groups was performed. All statistical significance for comparisons between groups including specific p-values and 95%-CI can be found in the appendix. Statistical differences are shown and marked as following: The chondrocytes group was statistically significantly higher than the arthroscopically and manually minced group. Picture b through f shows proliferated cells (green) and all cells (blue). (b) Chondral allograft biopsy; (c) Manually minced cartilage; (d) Arthroscopically minced cartilage; (e) Chondrons; (f) Chondrocytes. Isolated cell groups show a homogenous distribution of proliferated cells. Minced cartilage groups show less proliferation on the edges in comparison to the chondral allograft biopsy. All pictures taken at 20x magnification.
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    Proliferation of minced cartilage and isolated cells on day 28. A biopsy from a chondral allograft, minced cartilage minced manually or arthroscopically and isolated chondrons and chondrocytes were cultured in fibrin gel and proliferation was determined on day 28. (a) Graph showing the percentage of proliferated cells in minced cartilage and isolated cells on day 28. Each point represents a donor with a minimum of one replicate per donor. Each donor is represented by a different symbol. Standard deviations are shown. A GEE analysis with a Bonferroni post-hoc correction for multiple testing between all groups was performed. All statistical significance for comparisons between groups including specific p-values and 95%-CI can be found in the appendix. Statistical differences are shown and marked as following: The chondrocytes group was statistically significantly higher than the arthroscopically and manually minced group. Picture b through f shows proliferated cells (green) and all cells (blue). (b) Chondral allograft biopsy; (c) Manually minced cartilage; (d) Arthroscopically minced cartilage; (e) Chondrons; (f) Chondrocytes. Isolated cell groups show a homogenous distribution of proliferated cells. Minced cartilage groups show less proliferation on the edges in comparison to the chondral allograft biopsy. All pictures taken at 20x magnification.

    Journal: Osteoarthritis and Cartilage Open

    Article Title: Arthroscopically and manually minced cartilage demonstrates lower cell viability and lower proteoglycan deposition compared to isolated chondrons and chondrocytes

    doi: 10.1016/j.ocarto.2026.100843

    Figure Lengend Snippet: Proliferation of minced cartilage and isolated cells on day 28. A biopsy from a chondral allograft, minced cartilage minced manually or arthroscopically and isolated chondrons and chondrocytes were cultured in fibrin gel and proliferation was determined on day 28. (a) Graph showing the percentage of proliferated cells in minced cartilage and isolated cells on day 28. Each point represents a donor with a minimum of one replicate per donor. Each donor is represented by a different symbol. Standard deviations are shown. A GEE analysis with a Bonferroni post-hoc correction for multiple testing between all groups was performed. All statistical significance for comparisons between groups including specific p-values and 95%-CI can be found in the appendix. Statistical differences are shown and marked as following: The chondrocytes group was statistically significantly higher than the arthroscopically and manually minced group. Picture b through f shows proliferated cells (green) and all cells (blue). (b) Chondral allograft biopsy; (c) Manually minced cartilage; (d) Arthroscopically minced cartilage; (e) Chondrons; (f) Chondrocytes. Isolated cell groups show a homogenous distribution of proliferated cells. Minced cartilage groups show less proliferation on the edges in comparison to the chondral allograft biopsy. All pictures taken at 20x magnification.

    Article Snippet: To assess cell proliferation, samples were cultured with 5-ethynyl-2′-deoxyuridine (EdU, Invitrogen, Carlsbad, CA, USA).

    Techniques: Isolation, Cell Culture, Comparison