ethynyl Search Results


serum  (Thermo Fisher)
98
Thermo Fisher serum
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethylethynyl ether  (GFS Chemicals)
90
GFS Chemicals ethylethynyl ether
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Ethylethynyl Ether, supplied by GFS Chemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hy-118411  (medchemexpress)
95
medchemexpress hy-118411
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Hy 118411, supplied by medchemexpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethynyl  (Toronto Research Chemicals)
90
Toronto Research Chemicals ethynyl
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Ethynyl, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cas  (Selleck Chemicals)
93
Selleck Chemicals cas
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Cas, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5 ethynyl 2 deoxyuridine  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology 5 ethynyl 2 deoxyuridine
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
5 Ethynyl 2 Deoxyuridine, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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agn193109  (Toronto Research Chemicals)
85
Toronto Research Chemicals agn193109
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Agn193109, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aladdin scientific 61135 33 9  (Aladdin Scientific Corporation)
94
Aladdin Scientific Corporation aladdin scientific 61135 33 9
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Aladdin Scientific 61135 33 9, supplied by Aladdin Scientific Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jena bioscience clk n001 100  (Jena Bioscience)
95
Jena Bioscience jena bioscience clk n001 100
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Jena Bioscience Clk N001 100, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clk n002  (Jena Bioscience)
96
Jena Bioscience clk n002
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Clk N002, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleotide analog  (Tocris)
93
Tocris nucleotide analog
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
Nucleotide Analog, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethinyl estradiol  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ethinyl estradiol
Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h <t>serum</t> <t>starved</t> NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.
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Image Search Results


Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h serum starved NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.

Journal: The Journal of Biological Chemistry

Article Title: Mesothelin promotes acute myeloid leukemia progression through LYN-dependent signaling

doi: 10.1016/j.jbc.2026.111390

Figure Lengend Snippet: Characterization of MSLN -knockout (KO) in NOMO-1 and PDX AML001 cells. A , confirmation of MSLN-KO in two single-cell clones (2B2 – 1 bp insertion; 2E8 – 1 bp deletion) of NOMO-1 and PDX AML001 via flow cytometry staining with PE-conjugated anti-MSLN (Amatuximab). NOMO-1 2B2, 2E8, and PDX AML001 MSLN-KO had decreased cell surface MSLN expression as indicated by the peak shift to the left comparative to negative control. B , validation of MSLN-KO via western blotting analysis with vinculin or GAPDH serving as loading control, respectively. C , proliferation analysis of NOMO-1 (circles) and MSLN-KO (squares, 2B2; triangles, 2E8) over 96 h. Cell density was assessed via a hemocytometer following Trypan Blue staining. Mean ± standard deviation from 3 independent experiments in triplicates is plotted. ∗∗∗ p < 0.001. D , short-term NOMO-1 and MSLN-KO cell growth assessed using a Click-iT EdU assay after 24 h of serum starvation. Data from 3 independent experiments is plotted. ∗ p < 0.05, ∗∗ p < 0.01. E , cell cycle analysis on 24 h serum starved NOMO-1 and MSLN-KO cells. Representative flow cytometry plots are shown. Data from 3 independent experiments are graphed as mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01. F , Kaplan-Meier survival estimates of mice transplanted with NOMO-1 (n = 6), MSLN-KO (n = 8), PDX AML001 (n = 5) or MSLN-KO AML001 (n = 4) via i.v. injection. ∗ p < 0.05.

Article Snippet: Cells were serum-starved for 24 h, then incubated in complete media supplemented with 10 μM 5-ethynyl-2′-deoxyuridine (EdU) for 2 h before analysis with Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (ThermoFisher Scientific) according to manufacturer instructions.

Techniques: Knock-Out, Single Cell, Clone Assay, Flow Cytometry, Staining, Expressing, Negative Control, Biomarker Discovery, Western Blot, Control, Standard Deviation, EdU Assay, Cell Cycle Assay, Injection