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Huabio Inc p erk
P Erk, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erk1/2+anti+erk1/pm40407305-80-31-33
Average 86 stars, based on 1 article reviews
p erk - by Bioz Stars, 2026-09
86/100 stars

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Transfection:

Article Title: Arginase 1 promotes hepatic lipogenesis by regulating ERK2/PPARγ signaling in a non-canonical manner.
Article Snippet: .. Following transfection, the cells were collected by centrifugation at 800 g for 10 minutes at 4°C.Additionally, plasmids for full-length mouse Arg1, mutant Arg1, full-length ERK2, and various ERK2 mapping constructs were also designed and synthesized by HUABIO, China. ..

Centrifugation:

Article Title: Arginase 1 promotes hepatic lipogenesis by regulating ERK2/PPARγ signaling in a non-canonical manner.
Article Snippet: .. Following transfection, the cells were collected by centrifugation at 800 g for 10 minutes at 4°C.Additionally, plasmids for full-length mouse Arg1, mutant Arg1, full-length ERK2, and various ERK2 mapping constructs were also designed and synthesized by HUABIO, China. ..

Mutagenesis:

Article Title: Arginase 1 promotes hepatic lipogenesis by regulating ERK2/PPARγ signaling in a non-canonical manner.
Article Snippet: .. Following transfection, the cells were collected by centrifugation at 800 g for 10 minutes at 4°C.Additionally, plasmids for full-length mouse Arg1, mutant Arg1, full-length ERK2, and various ERK2 mapping constructs were also designed and synthesized by HUABIO, China. ..

Article Title: Arginase 1 promotes hepatic lipogenesis by regulating ERK2/PPARγ signaling in a non-canonical manner
Article Snippet: .. In addition, plasmids for full-length mouse Arg1, mutant Arg1, full-length ERK2, and various ERK2 mapping constructs were also designed and synthesized by HUABIO, China. ..

Construct:

Article Title: Arginase 1 promotes hepatic lipogenesis by regulating ERK2/PPARγ signaling in a non-canonical manner.
Article Snippet: .. Following transfection, the cells were collected by centrifugation at 800 g for 10 minutes at 4°C.Additionally, plasmids for full-length mouse Arg1, mutant Arg1, full-length ERK2, and various ERK2 mapping constructs were also designed and synthesized by HUABIO, China. ..

Article Title: Arginase 1 promotes hepatic lipogenesis by regulating ERK2/PPARγ signaling in a non-canonical manner
Article Snippet: .. In addition, plasmids for full-length mouse Arg1, mutant Arg1, full-length ERK2, and various ERK2 mapping constructs were also designed and synthesized by HUABIO, China. ..

Synthesized:

Article Title: Arginase 1 promotes hepatic lipogenesis by regulating ERK2/PPARγ signaling in a non-canonical manner.
Article Snippet: .. Following transfection, the cells were collected by centrifugation at 800 g for 10 minutes at 4°C.Additionally, plasmids for full-length mouse Arg1, mutant Arg1, full-length ERK2, and various ERK2 mapping constructs were also designed and synthesized by HUABIO, China. ..

Article Title: Arginase 1 promotes hepatic lipogenesis by regulating ERK2/PPARγ signaling in a non-canonical manner
Article Snippet: .. In addition, plasmids for full-length mouse Arg1, mutant Arg1, full-length ERK2, and various ERK2 mapping constructs were also designed and synthesized by HUABIO, China. ..

Incubation:

Article Title: Erbin Confers Neuroprotection Against Cerebral Ischemia-Reperfusion Injury in Mice via MAPK Pathway Inhibition
Article Snippet: Protein extraction was performed, and 251 the total protein concentration was determined using a commercial bicinchoninic acid 252 assay (BCA) kit (Yeasen Biotechnology, China), followed by subjecting the samples to 253 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or 4%-20% 254 gradient gel (ACE Biotechnology, China) followed by electro-transfer onto a 255 polyvinylidene fluoride (PVDF) membrane. .. 256 The membranes were incubated overnight at 4°C with the primary antibodies 257 against the following proteins: Erbin (1:1000, NBP2-56104, Novus, USA), ERK1/2 258 (1:500, ET1601-29, Huabio, China), p-ERK1/2 (1:1000, ET1610-13, Huabio, China), 259 JNK1/2/3 (1:500, ET1601-28, Huabio, China), p-JNK1/2/3 (1:500, ET1609-42, Huabio, 260 China), p38 (1:1000, ET1702-65, Huabio, China), p-p38 (1:1000, ER2001-52, Huabio, 261 China), β-tubulin (1:10000, ET1602-4, Huabio, China). ..

Article Title: The crucial role of RIG-I in upregulating neutrophil CXCR2 expression through the MAPK signaling pathway in viral pneumonia.
Article Snippet: Equal quantities of proteins were subjected to SDS-PAGE and transferred onto PVDF membranes (IPVH00010, Millipore Corporation, Billerica MA, USA). .. The membranes were blocked with a solution of 5% bovine serum albumin in TBST (0.1% Tween 20) before being incubated with primary antibodies including anti-human β-actin (1:1000, Cat: 66009-1-Ig, Proteintech), RIG-I(1:1000, Cat: NBP2-45678, Novus, Colorado, USA), MEK (1:1000, Cat: ET1602-3, Huabio, Zhejiang, China), phosphorylated MEK (1:1000, Cat: #9154, Cell Signaling Technology (CST)), ERK1/2 (1:1000, Cat: ET1601-29, Huabio), phosphorylated ERK1/2 (1:1000, Cat: ET1610-13, Huabio). .. After washing with TBST, the membranes were incubated with an HRP-conjugated secondary antibody (at a 1:10000 dilution, Proteintech) for 1 h at room temperature.



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Siglec-5 induces through the <t>HSF1/SYK/ERK1/2/SIRPα</t> signaling pathway. ( A) Western blot for detecting Siglec5, HSF1, NLRP3, p-SYK, p-ERK, p-ELK1, SIRPα, p-SHP2, and NOX4 protein expression. (B) Data statistics. Relative protein expression was quantified by densitometry using ImageJ and normalized to corresponding internal reference proteins (e.g., GAPDH or total protein). Data represent mean ± SD from three independent experiments. (C) CO-IP experiments confirmed the interaction; (C) Co-IP assays verified the interactions between Siglec5/HSF1 and ELK1/SIRPa.
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Image Search Results


Siglec-5 induces through the HSF1/SYK/ERK1/2/SIRPα signaling pathway. ( A) Western blot for detecting Siglec5, HSF1, NLRP3, p-SYK, p-ERK, p-ELK1, SIRPα, p-SHP2, and NOX4 protein expression. (B) Data statistics. Relative protein expression was quantified by densitometry using ImageJ and normalized to corresponding internal reference proteins (e.g., GAPDH or total protein). Data represent mean ± SD from three independent experiments. (C) CO-IP experiments confirmed the interaction; (C) Co-IP assays verified the interactions between Siglec5/HSF1 and ELK1/SIRPa.

Journal: Frontiers in Medicine

Article Title: Siglec-5 suppresses LPS-induced acute lung injury via negative regulation of HSF1/SYK-mediated ROS production and pyroptosis

doi: 10.3389/fmed.2026.1799874

Figure Lengend Snippet: Siglec-5 induces through the HSF1/SYK/ERK1/2/SIRPα signaling pathway. ( A) Western blot for detecting Siglec5, HSF1, NLRP3, p-SYK, p-ERK, p-ELK1, SIRPα, p-SHP2, and NOX4 protein expression. (B) Data statistics. Relative protein expression was quantified by densitometry using ImageJ and normalized to corresponding internal reference proteins (e.g., GAPDH or total protein). Data represent mean ± SD from three independent experiments. (C) CO-IP experiments confirmed the interaction; (C) Co-IP assays verified the interactions between Siglec5/HSF1 and ELK1/SIRPa.

Article Snippet: HSF1 inhibitor Rocaglamide, SYK inhibitor Piceatannol, ERK1/2 activator mSIRK, and ELK1 inhibitor TAT-DEF-Elk-1 (TDE) were purchased from MCE.

Techniques: Western Blot, Expressing, Co-Immunoprecipitation Assay

Siglec-5 inhibits LPS-induced lung injury by modulating the HSF1/SYK/ERK1/2/SIRPα signaling pathway to suppress ROS and pyroptosis responses.

Journal: Frontiers in Medicine

Article Title: Siglec-5 suppresses LPS-induced acute lung injury via negative regulation of HSF1/SYK-mediated ROS production and pyroptosis

doi: 10.3389/fmed.2026.1799874

Figure Lengend Snippet: Siglec-5 inhibits LPS-induced lung injury by modulating the HSF1/SYK/ERK1/2/SIRPα signaling pathway to suppress ROS and pyroptosis responses.

Article Snippet: HSF1 inhibitor Rocaglamide, SYK inhibitor Piceatannol, ERK1/2 activator mSIRK, and ELK1 inhibitor TAT-DEF-Elk-1 (TDE) were purchased from MCE.

Techniques: