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ecori-digested lambda vector  (Agilent technologies)


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    Agilent technologies ecori-digested lambda vector
    Ecori Digested Lambda Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecori-digested+lambda+vector/us07105324-297-16-20
    Average 90 stars, based on 1 article reviews
    ecori-digested lambda vector - by Bioz Stars, 2026-10
    90/100 stars

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    Plasmid Preparation:

    Article Title: VMP-like sequences of pathogenic
    Article Snippet: .. The preparation was then digested with EcoRI and ligated into EcoRI-treated λDASH II vector (Stratagene). ..

    Amplification:

    Article Title: XtracTB Assay, a Mycobacterium tuberculosis molecular screening test with sensitivity approaching culture
    Article Snippet: .. Synthetic DNA fragments (gBlocks) containing a portion of the cotJC gene of Bacillus atrophaeus were ordered from Integrated DNA Technologies (IDT, Coralville, IA, USA), and were amplified using primers with EcoRI restriction sites at the 5′ end of each primer (F primer: 5′-TTT TTG AAT TCT CAA TCA GCC ATT GGT AGG TC-3′; R primer: 5′-TTT TTG AAT TCA GCT GCA ATA TCC TGT AAA GGT C -3′) and ligated into Lambda Zap II vector predigested with EcoRI (Agilent, Santa Clara, CA, USA) using T4 DNA ligase (New England Biolabs, Ipswich MA, USA) following manufacturer’s instructions. .. Ligated vector was packaged into phages using Gigapack II Plus Packaging Extract (Agilent) following manufacturer’s instructions.

    other:

    Article Title: Chromosome compaction is triggered by an autonomous DNA-binding module within condensin.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Immobilon Crescendo Western HRP substrate Millipore Cat # WBLUR0100 ClarityTM Western ECL Substrate Bio-Rad Laboratories Cat # 1705060 Deposited data Raw and analyzed data This study Zenodo: https://doi.org/10.5281/zenodo.10699285 Experimental models: Organisms/strains Saccharomyces cerevisiae strain background W303: MATa/a leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 [phi+] This study, Table S3 N/A Oligonucleotides rDNA9 (Forward): 50- GGGGCCTAGTTTAGAGAGAAGTAGAC-30 Johzuka and Horiuchi57 N/A rDNA9 (Reverse): 50- GGGAAGCTTTGTGAAAGCCCTTCTCT-30 Johzuka and Horiuchi57 N/A rDNA10 (Forward): 50-GTGAAAGGATTTGCCCGGACAGTTTG-30 Johzuka and Horiuchi57 N/A rDNA10 (Reverse): 50-CCCACTGTTCACTGTTCACTGTTCAC-30 Johzuka and Horiuchi57 N/A rDNA11 (Forward): 50-AGGCAGCGTAAAAGGATGAGGCTACT-30 Johzuka and Horiuchi57 N/A rDNA11 (Reverse): 50-TTCCTTCCCGCTTTCCTGCACTAACA-30 Johzuka and Horiuchi57 N/A rDNA12 (Forward): 50-TGGAGGGAAGAGAACAAAACCGGAGT-30 Johzuka and Horiuchi57 N/A rDNA12 (Reverse): 50-ATACGCGAAACTCAGGTGCTGCAATC-30 Johzuka and Horiuchi57 N/A rDNA13 (Forward): 50-ATATGAGGGCAGGGTCCAGACATGTT-30 Johzuka and Horiuchi57 N/A rDNA13 (Reverse): 50-ACCTGTCACCTTGAAACTACCTCTGC-30 Johzuka and Horiuchi57 N/A Recombinant DNA FX174 virion ssDNA, 5386nt New England Biolabs Cat #N3023S EcoRI-digested pBluescript II KS dsDNA, 2961bp Agilent Cat # 212207 FX174 RF I DNA New England Biolabs Cat #N3021S pET SUMO Invitrogen Cat #K30001 pET-30(a) Novagen (Sigma) Cat # 69909 pRS316-RDN1 This study N/A Software and algorithms Fiji NIH https://imagej.nih.gov/ij/index.html GraphPad Prism 9.0 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ UCSF ChimeraX Pettersen et al.74 https://www.cgl.ucsf.edu/chimera/ PyMOL Schrödinger, LLC https://pymol.org/ IDPConformerGenerator platform Teixeira et al.28; Liu et al.29 https://github.com/julie-forman-kay- lab/IDPConformerGenerator AlphaFold Protein Structure Database Jumper et al.31; Varadi et al.30 https://alphafold.ebi.ac.uk/ NetSurfP-3.0 Høie et al.20 https://services.healthtech.dtu.dk/ services/NetSurfP-3.0/ PrDOS Ishida et al.21 https://prdos.hgc.jp/cgi-bin/top.cgi IUPred3 Erd}os et al.22 https://iupred3.elte.hu DisoRDPbind Peng et al.50 http://biomine.cs.vcu.edu/servers/DisoRDPbind/ IDR Bioinformatics Resource - Evolutionary Signatures Zarin et al.34 http://www.moseslab.csb.utoronto.ca/idr/ Cell Reports 43, 114419, July 23, 2024 19

    Article Title: Compounds and methods for diagnosis and treatment of leishmaniasis
    Article Snippet: The insert was subsequently ligated into the ZAP Express© vector predigested with EcoRI (Stratagene, La Jolla, Calif.) and packaged using Gigapack III© Gold Packaging Extract (Stratagene).

    Article Title: Molecular Cloning of an Immunogenic Protein of Baylisascaris procyonis and Expression in Escherichia coli for Use in Developing Improved Serodiagnostic Assays
    Article Snippet: The cDNA was synthesized using a ZAP-cDNA synthesis kit (Stratagene, La Jolla, CA) and cloned into bacteriophage lambda ZAP II vector digested with EcoRI and XhoI (Uni-ZAP XR vector; Stratagene) according to the manufacturer's suggested procedures.

    Ligation:

    Article Title: Methods of using genetic variants for the diagnosis and treatment of inflammatory bowel disease
    Article Snippet: Adapted inserts were then phosphorylated with T4 polynucleotide kinase (Stratagene) and were selected by size with a Sephacryl 400-HR column (Sigma-Aldrich). .. Approximately 0.25 μg of insert was ligated to 1.0 μg Lambda ZAP Express Vector treated with EcoRI and calf intestinal alkaline phosphatase (Stratagene), and the ligation mix was packaged with Gigapack III Gold packaging extract (Stratagene) following the manufacturer's instructions. ..

    Labeling:

    Article Title: Protein-deamidating enzyme, microorganism producing the same, gene encoding the same, production process therefor, and use thereof
    Article Snippet: .. The chromosomal DNA prepared in the above step a) was digested with EcoRI, and the thus obtained digest was ligated to an EcoRI-treated λ ZAPII (mfd. by Stratagene) vector and packaged using Gigapack III Gold (mfd. by Stratagene) to obtain the gene library. e) Screening of Gene Library The 0.48 kb DNA fragment obtained in the above step c) was labeled using Megaprime DNA Labeling System (mfd. by Amersham) and 32P-α-dCTP. ..

    DNA Labeling:

    Article Title: Protein-deamidating enzyme, microorganism producing the same, gene encoding the same, production process therefor, and use thereof
    Article Snippet: .. The chromosomal DNA prepared in the above step a) was digested with EcoRI, and the thus obtained digest was ligated to an EcoRI-treated λ ZAPII (mfd. by Stratagene) vector and packaged using Gigapack III Gold (mfd. by Stratagene) to obtain the gene library. e) Screening of Gene Library The 0.48 kb DNA fragment obtained in the above step c) was labeled using Megaprime DNA Labeling System (mfd. by Amersham) and 32P-α-dCTP. ..



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