bsiwi (New England Biolabs)
96
Structured Review
New England Biolabs
bsiwi
Bsiwi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori-digested+lambda+vector/BsiWI/custom%40r0553%4033463551
Average 96 stars, based on 315 article reviews
Bsiwi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori-digested+lambda+vector/BsiWI/custom%40r0553%4033463551
Average 96 stars, based on 315 article reviews
bsiwi - by Bioz Stars,
2026-10
96/100 stars
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Polymerase Chain Reaction:Article Title: A trypanosome trifecta: an independently tunable triple inducible system for genetic studies in Trypanosoma brucei Article Snippet: .. The neomycin resistance gene was PCR amplified from pC-PTP-NEO ( ) using primers UM184 and UM185 for subsequent Gibson assembly (NEB) with Article Title: A trypanosome trifecta: an independently tunable triple inducible system for genetic studies in Trypanosoma brucei . Article Snippet: .. Cumate inducible expression vectors The neomycin resistance gene was PCR amplified from pC-PTP-NEO (24) using primers UM184 and UM185 for subsequent Gibson assembly (NEB) with Article Title: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia. Article Snippet: The PDK1 ORF was amplified from pDONR223-PDK1 (Addgene, #23804) (primer pair PDK1_ORFstd_forw and PDK1_ORFstd_rev). .. A pGL4.13 plasmid (Promega, E6681), modified by introduction of a BsiWI restriction site downstream of the SV40 promoter, was digested with Amplification:Article Title: A trypanosome trifecta: an independently tunable triple inducible system for genetic studies in Trypanosoma brucei Article Snippet: .. The neomycin resistance gene was PCR amplified from pC-PTP-NEO ( ) using primers UM184 and UM185 for subsequent Gibson assembly (NEB) with Article Title: A trypanosome trifecta: an independently tunable triple inducible system for genetic studies in Trypanosoma brucei . Article Snippet: .. Cumate inducible expression vectors The neomycin resistance gene was PCR amplified from pC-PTP-NEO (24) using primers UM184 and UM185 for subsequent Gibson assembly (NEB) with Article Title: Rax1/2 promote memory of growth by early Ras1 activation to maintain polarity across generations Article Snippet: .. The amplified fragments were then assembled with either Expressing:Article Title: A trypanosome trifecta: an independently tunable triple inducible system for genetic studies in Trypanosoma brucei . Article Snippet: .. Cumate inducible expression vectors The neomycin resistance gene was PCR amplified from pC-PTP-NEO (24) using primers UM184 and UM185 for subsequent Gibson assembly (NEB) with Plasmid Preparation:Article Title: In vivo -directed evolution identifies AAV-WM04 as a next-generation vector for potent and durable hearing restoration in DFNB9 Article Snippet: .. The wild-type AAV2 capsid plasmid was linearized via Article Title: Leading and lagging strand abasic sites differentially affect vertebrate replisome progression but involve analogous bypass mechanisms Article Snippet: A plasmid designed to position the leading strand abasic site positioned 600 bp from the LacR array (pMC17) was commercially synthesized (Genscript) by inserting the oMC30/31 duplex in the reverse orientation at a position that places the damage site on the bottom strand 600 bp from the first lacO sequence of the array. .. To generate a plasmid containing a site-specific, but not strand-specific, AP site, pMC10 was digested with BsrGI and Article Title: Mapping the gene regulatory landscape of archaic hominin introgression in modern Papuans Article Snippet: .. The plasmid library was digested with Article Title: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia. Article Snippet: The PDK1 ORF was amplified from pDONR223-PDK1 (Addgene, #23804) (primer pair PDK1_ORFstd_forw and PDK1_ORFstd_rev). .. A pGL4.13 plasmid (Promega, E6681), modified by introduction of a BsiWI restriction site downstream of the SV40 promoter, was digested with Clone Assay:Article Title: Mapping the gene regulatory landscape of archaic hominin introgression in modern Papuans Article Snippet: .. The plasmid library was digested with Modification:Article Title: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia. Article Snippet: The PDK1 ORF was amplified from pDONR223-PDK1 (Addgene, #23804) (primer pair PDK1_ORFstd_forw and PDK1_ORFstd_rev). .. A pGL4.13 plasmid (Promega, E6681), modified by introduction of a BsiWI restriction site downstream of the SV40 promoter, was digested with Luciferase:Article Title: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia. Article Snippet: The PDK1 ORF was amplified from pDONR223-PDK1 (Addgene, #23804) (primer pair PDK1_ORFstd_forw and PDK1_ORFstd_rev). .. A pGL4.13 plasmid (Promega, E6681), modified by introduction of a BsiWI restriction site downstream of the SV40 promoter, was digested with Cloning:Article Title: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia. Article Snippet: The PDK1 ORF was amplified from pDONR223-PDK1 (Addgene, #23804) (primer pair PDK1_ORFstd_forw and PDK1_ORFstd_rev). .. A pGL4.13 plasmid (Promega, E6681), modified by introduction of a BsiWI restriction site downstream of the SV40 promoter, was digested with Article Title: Rax1/2 promote memory of growth by early Ras1 activation to maintain polarity across generations Article Snippet: .. The amplified fragments were then assembled with either |