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agilent-based dna microarrays  (Agilent technologies)


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    Structured Review

    Agilent technologies agilent-based dna microarrays
    Agilent Based Dna Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna-based+microarray/pmc06279330-45-12-11
    Average 90 stars, based on 1 article reviews
    agilent-based dna microarrays - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: LIN9, a Subunit of the DREAM Complex, Regulates Mitotic Gene Expression and Proliferation of Embryonic Stem Cells
    Article Snippet: .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts. .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts.

    Control:

    Article Title: LIN9, a Subunit of the DREAM Complex, Regulates Mitotic Gene Expression and Proliferation of Embryonic Stem Cells
    Article Snippet: .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts. .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts.

    Transfection:

    Article Title: LIN9, a Subunit of the DREAM Complex, Regulates Mitotic Gene Expression and Proliferation of Embryonic Stem Cells
    Article Snippet: .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts. .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts.

    shRNA:

    Article Title: LIN9, a Subunit of the DREAM Complex, Regulates Mitotic Gene Expression and Proliferation of Embryonic Stem Cells
    Article Snippet: .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts. .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts.

    Plasmid Preparation:

    Article Title: LIN9, a Subunit of the DREAM Complex, Regulates Mitotic Gene Expression and Proliferation of Embryonic Stem Cells
    Article Snippet: .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts. .. RNA was isolated from control cells and from cells transfected with the LIN9 specific shRNA plasmid and subjected to Agilent DNA microarrays monitoring more than 39,000 transcripts.

    Labeling:

    Article Title: Mechanisms of Resistance to Decitabine in the Myelodysplastic Syndrome
    Article Snippet: .. Amplicons from patients with MDS after relapse were labeled with the Cy5 dye and cohybridized against amplicons from patients at diagnosis labeled with the Cy3 dye on Agilent Technologies 4×44 K custom DNA microarrays (Agilent, Santa Clara, CA) as described previously . ..

    Article Title: The Flagellin FliC of Clostridium difficile Is Responsible for Pleiotropic Gene Regulation during In Vivo Infection
    Article Snippet: Groups of germ-free mice were orally challenged with either C. difficile R20291 or the fliC mutant. .. Bacterial RNA was recovered from the caeca at 14 h post-infection, transcribed into cDNA and then labeled for hybridization to DNA microarrays (Agilent) as described in the . .. When we compared the in vivo transcriptomic profiles of the R20291 C. difficile strain to its isogenic fliC mutant, we observed a total of 310 genes differentially expressed at 14 h post-infection.

    Biomarker Discovery:

    Article Title: Mechanisms of Resistance to Decitabine in the Myelodysplastic Syndrome
    Article Snippet: .. Amplicons from patients with MDS after relapse were labeled with the Cy5 dye and cohybridized against amplicons from patients at diagnosis labeled with the Cy3 dye on Agilent Technologies 4×44 K custom DNA microarrays (Agilent, Santa Clara, CA) as described previously . ..

    other:

    Article Title: TNFR2/BIRC3-TRAF1 signaling pathway as a novel NK cell immune checkpoint in cancer
    Article Snippet: The transcriptional landscape of these NK cell subsets was analyzed by Agilent-based DNA microarrays and statistical analyses were performed, attempting to select gene products mostly overexpressed in tumor beds (rather than blood), in cases of poor (rather than favorable) prognosis as well as in NKp30C (rather than AB) isoform profiles ( , Suppl.

    Article Title: Robust interlaboratory reproducibility of a gene expression signature measurement consistent with the needs of a new generation of diagnostic tools
    Article Snippet: The DNA microarrays were fabricated by Agilent Technologies according to specifications provided by Agendia BV.

    Hybridization:

    Article Title: Adenovirus type 5 exerts genome-wide control over cellular programs governing proliferation, quiescence, and survival
    Article Snippet: .. Samples were processed and hybridized to Agilent Whole Human 44 k DNA microarrays for 17 hours at 60°C in parallel staggered batches using the Agilent hybridization kit. .. Slides were washed in parallel according to the manufacturer's protocol, which included a final drying rinse in acetonitrile, and scanned in batch using an Agilent two color scanner.

    Article Title: The Flagellin FliC of Clostridium difficile Is Responsible for Pleiotropic Gene Regulation during In Vivo Infection
    Article Snippet: Groups of germ-free mice were orally challenged with either C. difficile R20291 or the fliC mutant. .. Bacterial RNA was recovered from the caeca at 14 h post-infection, transcribed into cDNA and then labeled for hybridization to DNA microarrays (Agilent) as described in the . .. When we compared the in vivo transcriptomic profiles of the R20291 C. difficile strain to its isogenic fliC mutant, we observed a total of 310 genes differentially expressed at 14 h post-infection.



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    IHC staining of the NQO1 protein on tissue <t>microarray</t> of cervical lesions. (A) NQO1 protein was weakly positive in cervical SCC. (B) NQO1 protein showed diffuse and strong cytoplasmic-positive staining in cervical SCC. (C) NQO1 protein was negative in normal cervical epithelia. (D) NQO1 protein was positive in SCC.
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    Image Search Results


    IHC staining of the NQO1 protein on tissue microarray of cervical lesions. (A) NQO1 protein was weakly positive in cervical SCC. (B) NQO1 protein showed diffuse and strong cytoplasmic-positive staining in cervical SCC. (C) NQO1 protein was negative in normal cervical epithelia. (D) NQO1 protein was positive in SCC.

    Journal: BMC Cancer

    Article Title: NQO1 overexpression is associated with poor prognosis in squamous cell carcinoma of the uterine cervix

    doi: 10.1186/1471-2407-14-414

    Figure Lengend Snippet: IHC staining of the NQO1 protein on tissue microarray of cervical lesions. (A) NQO1 protein was weakly positive in cervical SCC. (B) NQO1 protein showed diffuse and strong cytoplasmic-positive staining in cervical SCC. (C) NQO1 protein was negative in normal cervical epithelia. (D) NQO1 protein was positive in SCC.

    Article Snippet: DNA was extracted from paraffin-embedded cervical lesion specimens using the High Pure PCR Template Preparation kit (Cat.11796828001, Roche, Penzberg, Germany), and HPV detection and genotyping were performed using a PCR-based HPV-DNA microarray system (Biomedlab, Seoul, South Korea).

    Techniques: Immunohistochemistry, Microarray, Staining