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Sysmex Corporation diffquik staining kit
Diffquik Staining Kit, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diffquik+staining+kit/diffquik+kit/pmc06506732-46-9-12
Average 90 stars, based on 1 article reviews
diffquik staining kit - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Staining:

Article Title: Accelerated apoptosis of peripheral blood monocytes in Cebpb-deficient mice.
Article Snippet: .. Smears of mouse peripheral blood were stained using a DiffQuik Kit (Sysmex, Kobe, Japan), which is a modified Wright Giemsa staining system. .. Images were obtained using an Olympus BX43 microscope (Olympus, Tokyo, Japan) at 100 magnification.

Article Title: Internal radiotherapy with copper-64-diacetyl-bis (N4-methylthiosemicarbazone) reduces CD133+ highly tumorigenic cells and metastatic ability of mouse colon carcinoma.
Article Snippet: .. At day 7, colonies were stained with DiffQuik kit (Sysmex, Kobe, Japan), and the number of colonies containing more than 50 cells was counted to determine the survival fractions. ..

Article Title: Copper-64-diacetyl-bis (N4-methylthiosemicarbazone) accumulates in rich regions of CD133+ highly tumorigenic cells in mouse colon carcinoma.
Article Snippet: Introduction: Cu-diacetyl-bis (N-methylthiosemicarbazone) (Cu-ATSM) is a potential imaging agent of hypoxic tumor for use with PET.. Recent literature demonstrated that cancer cells expressing CD133, which is a frequently used marker for so-called cancer stem cells or cancer stem cell-like cells (collectively referred to here as CSCs), contribute to tumor's therapeutic resistance and metastasis ability.. Culturing under hypoxia is also reported to enlarge the proportion of CD133 cells, which would indicate survival advantage of CD133 cells under hypoxia.

Article Title: HN1-mediated activation of lipogenesis through Akt-SREBP signaling promotes hepatocellular carcinoma cell proliferation and metastasis.
Article Snippet: .. After incubation, the inserts were washed and stained with a DiffQuik kit (Sysmex Corp., Kobe, Japan). ..

Article Title: Liquid nitrogen vapor is comparable to liquid nitrogen for storage of cryopreserved human sperm: evidence from the characteristics of post-thaw human sperm.
Article Snippet: 74 75 76 77 78 79 80 Jingmei Hu, M.D., Shidou Zhao, Ph.D., Chengyan Xu, Ph.D., Lin Zhang, M.D., Shaoming Lu, Ph.D., Linlin Cui, Ph.D., Jinlong Ma, Ph.D., and Zi-Jiang Chen, Ph.D. a Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University; b National Research Center for Assisted Reproductive Technology and Reproductive Genetics; c The Key Laboratory for Reproductive Endocrinology of Ministry of Education; d Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan; and e Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 Objective: To compare the differences in the characteristics of post-thaw human sperm after storage in either liquid nitrogen (LN2; 196 C) or LN2 vapor ( 167 C).. Design: Experimental study.. Setting: University hospital.

Article Title: β-Catenin expression is associated with cell invasiveness in pancreatic cancer
Article Snippet: .. After incubation at 37°C, invaded cells were stained using DiffQuik staining kit (Sysmex, Kobe, Japan). ..

Article Title: Effects of cerulein on keratin 8 phosphorylation and perinuclear reorganization in pancreatic cancer cells: Involvement of downregulation of protein phosphatase 2A and alpha4.
Article Snippet: Toxicants can perturb cellular homeostasis by modifying phosphorylation-based signaling.. In the present study, we examined the effects of cerulein, an inducer of acute pancreatitis, on keratin 8 (K8) phosphorylation.. We found that cerulein dose-dependently induced K8 phosphorylation and perinuclear reorganization in PANC-1 cells, thus leading to migration and invasion.

Article Title: Different pancreatic cancer microenvironments convert iPSCs into cancer stem cells exhibiting distinct plasticity with altered gene expression of metabolic pathways.
Article Snippet: .. After 48 h of incubation at 37 °C and 5% CO2, the media were discarded, and inserts were washed with PBS and stained with DiffQuik kit (Sysmex, Japan) according to the instructions provided with BioCoatTM Matrigel Invasion Chamber. .. The invaded cells were counted and photographed by the FSX100 microscope (Olympus, Japan).

Modification:

Article Title: Accelerated apoptosis of peripheral blood monocytes in Cebpb-deficient mice.
Article Snippet: .. Smears of mouse peripheral blood were stained using a DiffQuik Kit (Sysmex, Kobe, Japan), which is a modified Wright Giemsa staining system. .. Images were obtained using an Olympus BX43 microscope (Olympus, Tokyo, Japan) at 100 magnification.

Incubation:

Article Title: HN1-mediated activation of lipogenesis through Akt-SREBP signaling promotes hepatocellular carcinoma cell proliferation and metastasis.
Article Snippet: .. After incubation, the inserts were washed and stained with a DiffQuik kit (Sysmex Corp., Kobe, Japan). ..

Article Title: β-Catenin expression is associated with cell invasiveness in pancreatic cancer
Article Snippet: .. After incubation at 37°C, invaded cells were stained using DiffQuik staining kit (Sysmex, Kobe, Japan). ..

Article Title: Different pancreatic cancer microenvironments convert iPSCs into cancer stem cells exhibiting distinct plasticity with altered gene expression of metabolic pathways.
Article Snippet: .. After 48 h of incubation at 37 °C and 5% CO2, the media were discarded, and inserts were washed with PBS and stained with DiffQuik kit (Sysmex, Japan) according to the instructions provided with BioCoatTM Matrigel Invasion Chamber. .. The invaded cells were counted and photographed by the FSX100 microscope (Olympus, Japan).

Microscopy:

Article Title: Liquid nitrogen vapor is comparable to liquid nitrogen for storage of cryopreserved human sperm: evidence from the characteristics of post-thaw human sperm.
Article Snippet: 74 75 76 77 78 79 80 Jingmei Hu, M.D., Shidou Zhao, Ph.D., Chengyan Xu, Ph.D., Lin Zhang, M.D., Shaoming Lu, Ph.D., Linlin Cui, Ph.D., Jinlong Ma, Ph.D., and Zi-Jiang Chen, Ph.D. a Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University; b National Research Center for Assisted Reproductive Technology and Reproductive Genetics; c The Key Laboratory for Reproductive Endocrinology of Ministry of Education; d Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan; and e Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 Objective: To compare the differences in the characteristics of post-thaw human sperm after storage in either liquid nitrogen (LN2; 196 C) or LN2 vapor ( 167 C).. Design: Experimental study.. Setting: University hospital.

Migration:

Article Title: Effects of cerulein on keratin 8 phosphorylation and perinuclear reorganization in pancreatic cancer cells: Involvement of downregulation of protein phosphatase 2A and alpha4.
Article Snippet: Toxicants can perturb cellular homeostasis by modifying phosphorylation-based signaling.. In the present study, we examined the effects of cerulein, an inducer of acute pancreatitis, on keratin 8 (K8) phosphorylation.. We found that cerulein dose-dependently induced K8 phosphorylation and perinuclear reorganization in PANC-1 cells, thus leading to migration and invasion.

Membrane:

Article Title: Effects of cerulein on keratin 8 phosphorylation and perinuclear reorganization in pancreatic cancer cells: Involvement of downregulation of protein phosphatase 2A and alpha4.
Article Snippet: Toxicants can perturb cellular homeostasis by modifying phosphorylation-based signaling.. In the present study, we examined the effects of cerulein, an inducer of acute pancreatitis, on keratin 8 (K8) phosphorylation.. We found that cerulein dose-dependently induced K8 phosphorylation and perinuclear reorganization in PANC-1 cells, thus leading to migration and invasion.

Light Microscopy:

Article Title: Effects of cerulein on keratin 8 phosphorylation and perinuclear reorganization in pancreatic cancer cells: Involvement of downregulation of protein phosphatase 2A and alpha4.
Article Snippet: Toxicants can perturb cellular homeostasis by modifying phosphorylation-based signaling.. In the present study, we examined the effects of cerulein, an inducer of acute pancreatitis, on keratin 8 (K8) phosphorylation.. We found that cerulein dose-dependently induced K8 phosphorylation and perinuclear reorganization in PANC-1 cells, thus leading to migration and invasion.



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Triangle Biomedical diffquik stain kit
Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with <t>DiffQuik</t> ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.
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Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with <t>DiffQuik</t> ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.
Diffquik Staining Kit, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diffquik+staining+kit/diffquik+kit/pmc06506732-46-9-12
Average 90 stars, based on 1 article reviews
diffquik staining kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with DiffQuik ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.

Journal: Cancers

Article Title: ALDH1A2 Is a Candidate Tumor Suppressor Gene in Ovarian Cancer

doi: 10.3390/cancers11101553

Figure Lengend Snippet: Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with DiffQuik ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.

Article Snippet: After 24 h, the membranes were fixed with 100% methanol and stained with a DiffQuik stain kit (Triangle Biomedical Sciences, Inc., Durham, NC, USA).

Techniques: Expressing, Invasion Assay, Transfection, Plasmid Preparation, Staining, Microscopy, Incubation, Fluorescence