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diff-quick staining solution  (Sysmex Corporation)

 
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    Sysmex Corporation diff-quick staining solution
    Diff Quick Staining Solution, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diff-quick+staining+solution/diff+quick+staining+solution/pm39768218-63-16-19
    Average 90 stars, based on 1 article reviews
    diff-quick staining solution - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Inhaled underground subway dusts may stimulate multiple pathways of cell death signals and disrupt immune balance.
    Article Snippet: In this study, we aimed to identify a toxic mechanism and the potential health effects of ambient dusts in an underground subway station.. At 24 h exposure to human bronchial epithelial (BEAS-2B) cells (0, 2.5, 10, and 40 μg/mL), dusts located within autophagosome-like vacuoles, whereas a series of autophagic processes appeared to be blocked.. The volume, potential and activity of mitochondria decreased in consistent with a condensed configuration, and the percentage of late apoptotic cells increased accompanying S phase arrest.

    Article Title: Twenty-eight-day repeated inhalation toxicity study of nano-sized lanthanum oxide in male sprague-dawley rats.
    Article Snippet: Although the use of lanthanum has increased in field of high-tech industry worldwide, potential adverse effects to human health and to the environment are largely unknown.. The present study aimed to investigate the potential toxicity of nano-sized lanthanum oxide (La2O3) following repeated inhalation exposure in male Sprague-Dawley rats.. Male rats were exposed nose-only to nano-sized La2O3 for 28 days (5 days/week) at doses of 0, 0.5, 2.5, and 10 mg/m.

    Article Title: GRPR Drives Metastasis via CRABP2 and FNDC4 Pathways in Lung Adenocarcinoma.
    Article Snippet: After 24 h of incubation, cells that had migrated through the pores were stained using the Diff-Quick staining solution (Sysmex, Kobe, Japan) and observed under a microscope.

    Article Title: Bone morphogenetic protein 4 provides cancer-supportive phenotypes to liver fibroblasts in patients with hepatocellular carcinoma.
    Article Snippet: Background Cancer-associated fibroblasts (CAFs) are essential constituents of cancer-supportive microenvironments.. The high incidence of hepatocellular carcinoma (HCC) in advanced fibrosis patients implies that fibroblasts have a promoting effect on HCC development.. We aimed to explore the regulators of phenotypes and function of CAFs in the liver.

    Article Title: GRPR Drives Metastasis via CRABP2 and FNDC4 Pathways in Lung Adenocarcinoma
    Article Snippet: After 24 h of incubation, cells that had migrated through the pores were stained using the Diff-Quick staining solution (Sysmex, Kobe, Japan) and observed under a microscope.

    Staining:

    Article Title: Anti-semaphorin 3A antibody and treatment of Alzheimer's disease and inflammatory immune diseases using same
    Article Snippet: .. Then, the chamber was immersed with a cell staining solution (Diff-Quick, SYSMEX INTERNATIONAL REAGENTS CO., LTD) for 10 minutes or more, followed by washing twice with ultrapure water and drying. ..

    Article Title: Applicability of Endobronchial Ultrasound and Virtual Bronchoscopic Navigation Combined with Rapid On-Site Evaluation in Diagnosing Peripheral Lung Lesions
    Article Snippet: .. Diff stain was applied for specimen staining (Diff-Quik; Sysmex Ltd. Kobe, Japan). ..

    Article Title: Genome‑wide DNA hypomethylation drives a more invasive pancreatic cancer phenotype and has predictive occult distant metastasis and prognosis potential.
    Article Snippet: .. After the removal of the non‐migratory cells, the cells in the upper chamber were fixed and permeabilized for 2 min at room temperature, and stained for 4 min at room temperature using a Diff‐Quick® simple and rapid staining solution (Sysmex Corporation) according to the manufacturer's protocol. .. A total of 5 random fields per well in four independent experiments were observed using a Keyence BZ‐X700 fluorescence microscope (Keyence Corporation) to assess cell migration.

    Diff-Quik:

    Article Title: Anti-semaphorin 3A antibody and treatment of Alzheimer's disease and inflammatory immune diseases using same
    Article Snippet: .. Then, the chamber was immersed with a cell staining solution (Diff-Quick, SYSMEX INTERNATIONAL REAGENTS CO., LTD) for 10 minutes or more, followed by washing twice with ultrapure water and drying. ..

    Article Title: Applicability of Endobronchial Ultrasound and Virtual Bronchoscopic Navigation Combined with Rapid On-Site Evaluation in Diagnosing Peripheral Lung Lesions
    Article Snippet: .. Diff stain was applied for specimen staining (Diff-Quik; Sysmex Ltd. Kobe, Japan). ..

    Article Title: Genome‑wide DNA hypomethylation drives a more invasive pancreatic cancer phenotype and has predictive occult distant metastasis and prognosis potential.
    Article Snippet: .. After the removal of the non‐migratory cells, the cells in the upper chamber were fixed and permeabilized for 2 min at room temperature, and stained for 4 min at room temperature using a Diff‐Quick® simple and rapid staining solution (Sysmex Corporation) according to the manufacturer's protocol. .. A total of 5 random fields per well in four independent experiments were observed using a Keyence BZ‐X700 fluorescence microscope (Keyence Corporation) to assess cell migration.



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    Effect of IL-13Rα2 on migration and invasion activity of osteosarcoma cells: ( A ) Migration assay was conducted by seeding 1 × 10 5 cells into the upper chamber of each well. ( B ) Invasion assays were performed by seeding 2 × 10 5 cells in each well of the Matrigel-coated upper chamber. Serum-free media was used in the upper chamber for both migration and invasion assays, while the lower chamber contained media with 10% serum to serve as a chemoattractant. The migrated or invaded cells were <t>stained</t> with <t>Diff</t> <t>Quick</t> staining <t>solution</t> and counted in five separate ×100 microscopic fields per well. Original magnification, ×400. *** p < 0.001; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2.
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    Effect of IL-13Rα2 on migration and invasion activity of osteosarcoma cells: ( A ) Migration assay was conducted by seeding 1 × 10 5 cells into the upper chamber of each well. ( B ) Invasion assays were performed by seeding 2 × 10 5 cells in each well of the Matrigel-coated upper chamber. Serum-free media was used in the upper chamber for both migration and invasion assays, while the lower chamber contained media with 10% serum to serve as a chemoattractant. The migrated or invaded cells were stained with Diff Quick staining solution and counted in five separate ×100 microscopic fields per well. Original magnification, ×400. *** p < 0.001; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2.

    Journal: Pharmaceuticals

    Article Title: IL-13Rα2 Is Involved in Resistance to Doxorubicin and Survival of Osteosarcoma Patients

    doi: 10.3390/ph17111526

    Figure Lengend Snippet: Effect of IL-13Rα2 on migration and invasion activity of osteosarcoma cells: ( A ) Migration assay was conducted by seeding 1 × 10 5 cells into the upper chamber of each well. ( B ) Invasion assays were performed by seeding 2 × 10 5 cells in each well of the Matrigel-coated upper chamber. Serum-free media was used in the upper chamber for both migration and invasion assays, while the lower chamber contained media with 10% serum to serve as a chemoattractant. The migrated or invaded cells were stained with Diff Quick staining solution and counted in five separate ×100 microscopic fields per well. Original magnification, ×400. *** p < 0.001; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2.

    Article Snippet: Cells that migrated or invaded to the lower side of the membrane were stained with Diff Quick stain solution (#468.037.1A, Diff-Quick, Dade Behring Inc., Newark, DE, USA).

    Techniques: Migration, Activity Assay, Staining, Diff-Quik, Plasmid Preparation, Control, shRNA