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Nanjing Jiancheng Bioengineering Research Institute Co Ltd 3,3′-diamino benzidine solution (dab
3,3′ Diamino Benzidine Solution (Dab, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diamino-benzidine+solution/tetramethylbenzidine++tmb++chromogenic+reagent/pmc10550778-149-10-14
Average 90 stars, based on 1 article reviews
3,3′-diamino benzidine solution (dab - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Strategies for understanding the role of cellular heterogeneity in the pathogenesis of lung cancer: a cell model for chronic exposure to cigarette smoke extract
Article Snippet: We detected specific target proteins with a 3.3′-diaminobenzidine peroxidase substrate kit (Nanjing Jiancheng Bioengineering Institute).

Article Title: Induction of attenuated Nocardia seriolae and their use as live vaccine trials against fish nocardiosis.
Article Snippet: Nocardia seriolae, a Gram-positive facultative intercellular pathogen, has been identified as the causative agent of fish nocardiosis, causing substantial mortality and morbidity of a wide range of fish species.. Looking into that fact, the effective vaccine against this pathogen is urgently needed to control the significant losses in aquaculture practices.. In order to induct attenuated strains for developing the potential live vaccines, the mutagenic N. seriolae strain S-250 and U-20 were obtained from wild-type strain ZJ0503 through continuous passaging and ultraviolet (UV) irradiation, respectively.

Article Title: Design and synthesis of pterostilbene derivatives bearing triazole moiety that might treat DSS-induced colitis in mice through modulation of NF-κB/MAPK signaling pathways.
Article Snippet: In this study, a series of novel anti-inflammatory compounds with high activity and low toxicity were designed and synthesized based on the natural product pterostilbene skeleton.. According to the strategy of pharmacophore combination, we introduced thiazole moiety into pterostilbene skeleton to design and synthesize a novel series of pterostilbene derivatives (a total of 41 compounds), and lipopolysaccharide (LPS)-treated RAW 264.7 cells were screened for anti-inflammatory activity and cytotoxicity.. Among them, compound 8 was found to be the most active (against NO: IC50 = 0.6 μM) compared with pterostilbene and indomethacin.

Staining:

Article Title: Melatonin-loaded self-healing hydrogel targets mitochondrial energy metabolism and promotes annulus fibrosus regeneration
Article Snippet: The next day, the sections were incubated with a biotinylated secondary antibody (Abcam) for 1 h at room temperature. .. The sections were stained using an SP immunohistochemistry kit and 3,3′-diamino benzidine solution (DAB; Nanjing Jiancheng Bioengineering Institute, China) to visualize immunoreactivity. ..

Immunohistochemistry:

Article Title: Melatonin-loaded self-healing hydrogel targets mitochondrial energy metabolism and promotes annulus fibrosus regeneration
Article Snippet: The next day, the sections were incubated with a biotinylated secondary antibody (Abcam) for 1 h at room temperature. .. The sections were stained using an SP immunohistochemistry kit and 3,3′-diamino benzidine solution (DAB; Nanjing Jiancheng Bioengineering Institute, China) to visualize immunoreactivity. ..

Blocking Assay:

Article Title: Interleukin-12 exacerbates symptoms in an MRL/MpJ-Faslpr mouse model of systemic lupus erythematosus
Article Snippet: Briefly, 96-well microtiter plates were coated with 50 μg/ml calf thymus dsDNA (Sigma-Aldrich; Merck KGaA) overnight at 4 ̊C. .. After blocking with 1%BSA (Sigma-Aldrich; Merck KGaA) in PBS at room temperature for 1 h, 100-fold diluted serum (100 μl) was added, followed by incubation at room temperature for 2 h. Subsequently, goat anti-mouse antibody conjugated to horseradish peroxidase (1:2,000; cat. no. SA00001-1; Proteintech Group, Inc.) was added, followed by incubation at room temperature for 1 h. Next, 3,3',5,5'-Tetramethylbenzidine substrate solution (cat. no. B019-1-1; Nanjing Jiancheng Bioengineering Institute)was added, and plates were incubated at room temperature for 30 min. ..

Incubation:

Article Title: Interleukin-12 exacerbates symptoms in an MRL/MpJ-Faslpr mouse model of systemic lupus erythematosus
Article Snippet: Briefly, 96-well microtiter plates were coated with 50 μg/ml calf thymus dsDNA (Sigma-Aldrich; Merck KGaA) overnight at 4 ̊C. .. After blocking with 1%BSA (Sigma-Aldrich; Merck KGaA) in PBS at room temperature for 1 h, 100-fold diluted serum (100 μl) was added, followed by incubation at room temperature for 2 h. Subsequently, goat anti-mouse antibody conjugated to horseradish peroxidase (1:2,000; cat. no. SA00001-1; Proteintech Group, Inc.) was added, followed by incubation at room temperature for 1 h. Next, 3,3',5,5'-Tetramethylbenzidine substrate solution (cat. no. B019-1-1; Nanjing Jiancheng Bioengineering Institute)was added, and plates were incubated at room temperature for 30 min. ..

Article Title: Eucalyptol prevents bleomycin-induced pulmonary fibrosis and M2 macrophage polarization.
Article Snippet: Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, fibrosing interstitial pneumonia with limited therapeutic options.. Eucalyptol, a terpenoid oxide isolated from eucalyptus species, reportedly exhibits various biological activities such as anti-inflammatory and antioxidant effects.. In the present study, we aimed to determine whether eucalyptol could alleviate bleomycin (BLM)-induced pulmonary fibrosis and inhibit interleukin (IL)-13-induced M2 macrophage polarization.



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