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full-iterative deconvolution based on an experimental point-spread function softworx software  (Softworx Inc)

 
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    Structured Review

    Softworx Inc full-iterative deconvolution based on an experimental point-spread function softworx software
    Full Iterative Deconvolution Based On An Experimental Point Spread Function Softworx Software, supplied by Softworx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+point+spread+function/softworx+deconvolution+plugin/pm16249234-140-2-9
    Average 90 stars, based on 1 article reviews
    full-iterative deconvolution based on an experimental point-spread function softworx software - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Dual role of Mic10 in mitochondrial cristae organization and ATP synthase-linked metabolic adaptation and respiratory growth
    Article Snippet: Fluorescence microscopy images were recorded with a DeltaVision Ultra High Resolution microscope (GE Healthcare, Applied Precision) equipped with an UPlanSApo 100x/1.4 oil objective (Olympus), an sCMOS pco.edge camera (PCO), and a seven channel solid state light source (Lumencor). .. Raw microscopy images were deconvolved using the softWorX deconvolution plugin. ..

    Article Title: Global kinome profiling reveals DYRK1A as critical activator of the human mitochondrial import machinery
    Article Snippet: Fluorescent microscopy images were recorded with a DeltaVision Ultra High Resolution Microscope with UPlanSApo 100x/1.4 oil Olympus objective, using a sCMOS pro.edge camera at 37 °C. .. Raw fluorescent microscopy images were deconvolved at the DeltaVision microscope using SoftWorx deconvolution plugin. .. Quantification of morphological parameters of mitochondria was performed with the ImageJ 1.52 software (Wayne Rasband, National Institutes of Health, USA), using the Analyze Particles functionality .

    Article Title: Decoding the function of Atg13 phosphorylation reveals a role of Atg11 in bulk autophagy initiation.
    Article Snippet: Fluorescence microscopy images were recorded with a DeltaVision Ultra High-Resolution microscope (GE Healthcare, Applied Precision) equipped with an UPlanSApo 100x/1.4 oil objective (Olympus), a sCMOS pco.edge camera (PCO), and a seven-channel solid state light source (Lumencor). .. Raw microscopy images were deconvolved using the softWorX deconvolution plugin (version 7.2.1). ..

    Article Title: Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities
    Article Snippet: Cells were blocked in 3% BSA in PBS-T for 30 min before incubation with GFP-Booster _Atto488 (1:300; Chromotek), or mouse anti-G3BP1 in 3% BSA in PBS-T for 1 h at room temperature. .. Unbound primary antibodies were removed by washing four times for 5 min in PBS-T at room temperature followed by incubation with secondary antibodies (Alexa Fluor 546; 1:1000; Invitrogen) and DAPI for 45 min. Ibid dishes were then washed four times for 5 min in PBS-T. Z stacks 200 nm apart were recorded on a microscope (DeltaVision Elite) using a 40 × oil objective lens (numerical aperture 1.35) followed by deconvolution using SoftWoRx. ..

    Article Title: Spatial control of avidity regulates initiation and progression of selective autophagy.
    Article Snippet: Fluorescent microscopy images were recorded with a DeltaVision Ultra High Resolution microscope (GE Healthcare, Applied Precision) equipped with an UPlanSApo 100x/1.4 oil objective (Olympus), an sCMOS pco.edge camera (PCO), and a seven channel solid state light source (Lumencor) (Figs. 1a, b, d–g, 2a, b, and Supplementary Figs. 1a–d, 2a, b, 3b, 4d, e, 5c, d, 6d, 8b, e, f, 9b, c); or with a PersonalDeltaVision microscope (GE Healthcare, Applied Precision) equipped with an UPlanSApo 100× oil/1.4 oil objective (Olympus), a CoolSNAP HQ2 Monochrome CCD camera (Photometrics), and a seven-color InsightSSI solid state illumination unit (GE Healthcare, Applied Precision) (Fig. 2f and Supplementary Fig. 3a); or with an AxioObserver Z1 inverted microscope (ZEISS) equipped with an EC Plan-Neofluar 100x/1.3 oil M27 objective (ZEISS), a CoolSnap HQ2 Monochrome CCD camera (Photometrics), and a SOLA 6-LCR-SB light source (Lumencor) with the VisiView software (Visitron Systems) (Figs. 3c–g, 4b–e, g, 5a–d, 6a–e, 7a, b, and Supplementary Figs. 4b, c, 5a, b, e–g, 6a–c, e, 7a, b, 8a, c, d, 9a). .. Raw microscopy images acquired with the PersonalDeltaVision microscope or the DeltaVision Ultra High Resolution microscope were deconvolved using the softWorX deconvolution plugin (version R6.1.1 and version 7.2.1, respectively). ..

    Software:

    Article Title: Deletion of a Golgi protein in Trypanosoma cruzi reveals a critical role for Mn 2+ in protein glycosylation needed for host cell invasion and intracellular replication
    Article Snippet: .. Differential interference contrast and fluorescent optical images were acquired with a 100× objective (1.35 aperture) under non-saturating conditions with a Photometrix CoolSnapHQ charge-coupled device camera driven by DeltaVision software (Applied Precision, Issaquah, WA) and deconvolved for 15 cycles using Softworx deconvolution software. ..

    Incubation:

    Article Title: Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities
    Article Snippet: Cells were blocked in 3% BSA in PBS-T for 30 min before incubation with GFP-Booster _Atto488 (1:300; Chromotek), or mouse anti-G3BP1 in 3% BSA in PBS-T for 1 h at room temperature. .. Unbound primary antibodies were removed by washing four times for 5 min in PBS-T at room temperature followed by incubation with secondary antibodies (Alexa Fluor 546; 1:1000; Invitrogen) and DAPI for 45 min. Ibid dishes were then washed four times for 5 min in PBS-T. Z stacks 200 nm apart were recorded on a microscope (DeltaVision Elite) using a 40 × oil objective lens (numerical aperture 1.35) followed by deconvolution using SoftWoRx. ..



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    Image Search Results


    Upper row: examples of three experimental fluorescence images (cross-sections from three different F2-Z-stacks). Bottom row: results of the deconvolution of corresponding F2-Z-stack fluorescence images (white) and of the filling procedures convex hull (red contour) and alpha shape (blue contour). The scale bars represent 2 µm.

    Journal: Biomedical Optics Express

    Article Title: Method for nanoparticles uptake evaluation based on double labeled fluorescent cells scanned in enhanced darkfield microscopy

    doi: 10.1364/BOE.490136

    Figure Lengend Snippet: Upper row: examples of three experimental fluorescence images (cross-sections from three different F2-Z-stacks). Bottom row: results of the deconvolution of corresponding F2-Z-stack fluorescence images (white) and of the filling procedures convex hull (red contour) and alpha shape (blue contour). The scale bars represent 2 µm.

    Article Snippet: For F1-Z- and F2-Z-stacks, the point spread function and deconvolution routines provided by the CytoViva producer were run while for WL-Z-stacks, the built-in specific plugin “Just locate nanoparticles” was used [ 46 ].

    Techniques: Fluorescence