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Structured Review

Synthego Inc ice deconvolution algorithm
Ice Deconvolution Algorithm, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deconvolution+algorithms/algorithm+deconvolution+ice/pm41419196-192-14-17
Average 86 stars, based on 1 article reviews
ice deconvolution algorithm - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Produced:

Article Title: Glycerophosphodiesters inhibit lysosomal phospholipid catabolism in Batten disease
Article Snippet: .. Synthego ICE indel deconvolution web-based tool ( https://synthego.com ) was then used to determine the indels that were produced at the sgRNA target site. ..

Sequencing:

Article Title: NEUROG3 Is Sufficient to Drive Neuroendocrine Differentiation in Prostate Cancer Cells
Article Snippet: .. Sequence analysis confirmed indels at the target site, and Synthego ICE deconvolution verified high editing efficiency and frameshift disruption resulting in premature stop codons (Supplementary Figure S3A). ..

Article Title: A protective cGAMP-mediated anti-tumor immune response can proceed without LRRC8/VRAC channels
Article Snippet: Cells were transfected using Lipofectamine 2000 (Invitrogen, 11668019), and single-cell FACS of GFP-positive cells was performed with a FACSAria II or FACSAria III (BD Bioscience) device. .. Gene editing in arising monoclonal cell lines was validated by Sanger sequencing using the ICE deconvolution algorithm (Synthego). .. Target sequences specific for Lrrc8a and Cgas ( ) were designed with the Benchling software and cloned into PX458 vector (Addgene, 48138) expressing GFP, Cas9 and a single guide RNA (gRNA).

Disruption:

Article Title: NEUROG3 Is Sufficient to Drive Neuroendocrine Differentiation in Prostate Cancer Cells
Article Snippet: .. Sequence analysis confirmed indels at the target site, and Synthego ICE deconvolution verified high editing efficiency and frameshift disruption resulting in premature stop codons (Supplementary Figure S3A). ..

other:

Article Title: A protective cGAMP-mediated anti-tumor immune response can proceed without LRRC8/VRAC channels.
Article Snippet: Cell lines and culture conditions MC38 (provided by T. Blankenstein, MDC, Berlin) and B16-F10 (ATCC, #CRL6475) cells were cultured in RPMI 1640 (PAN Biotech, P04–16500) or DMEM (PAN Biotech, P04–03550), respectively.

CRISPR:

Article Title: PTPN2-KO CAR-T Cells Demonstrate Enhanced Effector Function, CNS Infiltration, and Toxicity in a Non-Human Primate CAR-T Model
Article Snippet: .. We first identified a CRISPR/Cas9 guide that would selectively and effectively delete PTPN2 in primary human T-cells in a region with low homology to PTPN1 ( Supplemental Fig.1A-B ), achieving a mean editing efficiency of 73.5+/-2.9% in primary T and CAR-T cells measured by interference analysis using Synthego ICE deconvolution ( , Supplemental Fig.1A) and transcriptional analysis (Supplemental Fig.1B) . ..

Analysis:

Article Title: PTPN2-KO CAR-T Cells Demonstrate Enhanced Effector Function, CNS Infiltration, and Toxicity in a Non-Human Primate CAR-T Model
Article Snippet: .. We first identified a CRISPR/Cas9 guide that would selectively and effectively delete PTPN2 in primary human T-cells in a region with low homology to PTPN1 ( Supplemental Fig.1A-B ), achieving a mean editing efficiency of 73.5+/-2.9% in primary T and CAR-T cells measured by interference analysis using Synthego ICE deconvolution ( , Supplemental Fig.1A) and transcriptional analysis (Supplemental Fig.1B) . ..



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