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dapi staining solution  (Beyotime)


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    Structured Review

    Beyotime dapi staining solution
    Reagents and tools table
    Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+solution/DAPI+Staining+Solution/pmc13650158-92-0-4
    Average 99 stars, based on 2060 article reviews
    dapi staining solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding"

    Article Title: Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding

    Journal: EMBO Reports

    doi: 10.1038/s44319-026-00911-4

    Reagents and tools table
    Figure Legend Snippet: Reagents and tools table

    Techniques Used: Recombinant, Magnetic Beads, Sequencing, Microscopy, Transfection, Plasmid Preparation, Staining, 96 Well Plates, Protease Inhibitor, Purification, SYBR Green Assay, Software, Mass Spectrometry, Transmission Assay

    Related Articles

    Staining:

    Article Title: TRPC6-TXNIP-NLRP3 signaling axis is involved in type 2 diabetes-associated cognitive dysfunction and the intervention of ginsenoside Rg1.
    Article Snippet: Type 2 diabetes-associated cognitive dysfunction (TDACD) poses a significant global public health challenge.. However, the core molecular mechanisms underlying its pathogenesis remain incompletely understood, and the development of effective therapeutic interventions continues to face considerable obstacles.. Here, we identify the role of the TRPC6-TXNIP-NLRP3 signaling axis in TDACD and to investigate the protective effects and mechanisms of ginsenoside Rg1 against TDACD.

    Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
    Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

    Article Title: Targeted delivery of lupeol via hyaluronic acid-modified ZIF-8 for anti-hepatic fibrosis therapy
    Article Snippet: Quantitative analysis was performed using flow cytometry (Beckman Coulter, USA). .. A DAPI Staining Solution (Beyotime Biotechnology, Shanghai, China) was used to perform the cell apoptosis shape assay on LX-2 cells according to the manufacturer's instructions. .. Cells were seeded into six-well plates at a density of 1.0 × 10 6 cells per well and cultured for 24 h. The next day, cells were treated with different media containing Lupeol, Lupeol@ZIF-8 and HA/Lupeol@ZIF-8 (60 μM lupeol), with blank and model groups included as controls, and incubated for an additional 24 h. For qualitative analysis, DAPI solution was added 24 h after treatment, and fluorescence signals were recorded using a fluorescence microscope (Olympus, Tokyo, Japan).

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    Article Title: Osthole promotes cell senescence-like phenotype to enhance radiosensitivity in non-small cell lung carcinoma possibly through modulating the Sirt1/NF-κB pathway
    Article Snippet: Background Radioresistance is a major problem in non-small cell lung carcinoma (NSCLC) treatment.. Osthole is a natural coumarin with antitumor effects.. This research sought to clarify if Osthole enhances the sensitivity of NSCLC to radiotherapy and to uncover the underlying mechanisms involved.

    Article Title: Development of perilla essential oil-based nanoemulsions as anti-psoriatic and dermal delivery platforms for topical therapy
    Article Snippet: PEG-40 stearyl ether (S40) was obtained from Croda GmbH (Nett et al., Germany). .. DAPI staining solution, RIPA buffer, hematoxylin and eosin (H&E) staining kit, and bicinchoninic acid (BCA) protein assay kits were obtained from Beyotime Biotechnology (Shanghai, China). .. Antibodies against Ki67, TNF-α, IL-6, IL-1β, IL-17 and a one-step TUNEL assay kit were from Servicebio (Wuhan, China).

    Article Title: Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding
    Article Snippet: Vector TrueVIEW kit , Vector laboratories , SP-8400. .. DAPI Staining Solution , Beyotime , C1006. .. mTeSR1 medium , STEMCELL , 85850.

    Article Title: EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT
    Article Snippet: .. Finally, nuclei were stained with DAPI staining solution (Beyotime, C1005). .. The expression intensity of target proteins was observed under a CSU-X1 laser confocal microscope (Nikon, Japan).

    ROS Assay:

    Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
    Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

    Membrane:

    Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
    Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    Immunostaining:

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    Blocking Assay:

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    BIA-KA:

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    Protein Concentration:

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    TUNEL Assay:

    Article Title: Osthole promotes cell senescence-like phenotype to enhance radiosensitivity in non-small cell lung carcinoma possibly through modulating the Sirt1/NF-κB pathway
    Article Snippet: Background Radioresistance is a major problem in non-small cell lung carcinoma (NSCLC) treatment.. Osthole is a natural coumarin with antitumor effects.. This research sought to clarify if Osthole enhances the sensitivity of NSCLC to radiotherapy and to uncover the underlying mechanisms involved.

    Incubation:

    Article Title: Osthole promotes cell senescence-like phenotype to enhance radiosensitivity in non-small cell lung carcinoma possibly through modulating the Sirt1/NF-κB pathway
    Article Snippet: Background Radioresistance is a major problem in non-small cell lung carcinoma (NSCLC) treatment.. Osthole is a natural coumarin with antitumor effects.. This research sought to clarify if Osthole enhances the sensitivity of NSCLC to radiotherapy and to uncover the underlying mechanisms involved.

    Bicinchoninic Acid Protein Assay:

    Article Title: Development of perilla essential oil-based nanoemulsions as anti-psoriatic and dermal delivery platforms for topical therapy
    Article Snippet: PEG-40 stearyl ether (S40) was obtained from Croda GmbH (Nett et al., Germany). .. DAPI staining solution, RIPA buffer, hematoxylin and eosin (H&E) staining kit, and bicinchoninic acid (BCA) protein assay kits were obtained from Beyotime Biotechnology (Shanghai, China). .. Antibodies against Ki67, TNF-α, IL-6, IL-1β, IL-17 and a one-step TUNEL assay kit were from Servicebio (Wuhan, China).



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    Image Search Results


    Reagents and tools table

    Journal: EMBO Reports

    Article Title: Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding

    doi: 10.1038/s44319-026-00911-4

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: DAPI Staining Solution , Beyotime , C1006.

    Techniques: Recombinant, Magnetic Beads, Sequencing, Microscopy, Transfection, Plasmid Preparation, Staining, 96 Well Plates, Protease Inhibitor, Purification, SYBR Green Assay, Software, Mass Spectrometry, Transmission Assay