dapi (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
dapi
Dapi, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining+solution+c1002/DAPI/pm38969190-76-38-16
Average 96 stars, based on 1459 article reviews
Dapi, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining+solution+c1002/DAPI/pm38969190-76-38-16
Average 96 stars, based on 1459 article reviews
dapi - by Bioz Stars,
2026-11
96/100 stars
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Staining:Article Title: Exposure of Human Corneal Epithelial Cells to Microplastic Particles Induces a Phase-Specific Cytokine Response. Article Snippet: 37 Microplastics have been identified in ophthalmic therapeutics and linked to dry eye 38 disease and meibomian gland dysfunction.. Inflammation is hypothesised to drive 39 ocular surface damage.. This in-vitro study investigates how varying concentrations of 40 microplastics affect corneal cell viability and cytokine expression. Article Title: CD200 + fibroblasts prime the immunoregulatory function of granulocytic myeloid-derived suppressor cells in inflammatory arthritis. Article Snippet: .. Secondary antibodies were stained for 1 h at room temperature followed by incubation with Article Title: Macrophage-derived IL-6 reprograms lipid metabolism to promote colorectal cancer development through USP14-mediated FASN deubiquitination Article Snippet: For immunohistochemistry, the slices were incubated with antibodies against IL-6 (Cat#AF-206-NA, R&D Systems, Minneapolis, Minnesota, USA), p-STAT3 (Cell Signaling Technology, Danvers, Massachusetts, USA), USP14 (Proteintech, Wuhan, Hubei, China) and FASN (Cat#10624-2-AP, Proteintech) and then treated with an HRP-labeled secondary antibody (Proteintech). .. After the final round of staining, Article Title: Glycine receptors in circulating white blood cells regulated by neuroinflammation Article Snippet: After incubation, the slides were washed three times with PBS for 5 minutes each, then incubated with appropriate secondary antibodies, i.e., Alexa Fluor 488 (1:200) and/or 594 (1:200) (Thermo-Fisher Scientific) for 1 hour at room temperature. .. A Fluorescence:Article Title: Exposure of Human Corneal Epithelial Cells to Microplastic Particles Induces a Phase-Specific Cytokine Response. Article Snippet: 37 Microplastics have been identified in ophthalmic therapeutics and linked to dry eye 38 disease and meibomian gland dysfunction.. Inflammation is hypothesised to drive 39 ocular surface damage.. This in-vitro study investigates how varying concentrations of 40 microplastics affect corneal cell viability and cytokine expression. Microscopy:Article Title: Exposure of Human Corneal Epithelial Cells to Microplastic Particles Induces a Phase-Specific Cytokine Response. Article Snippet: 37 Microplastics have been identified in ophthalmic therapeutics and linked to dry eye 38 disease and meibomian gland dysfunction.. Inflammation is hypothesised to drive 39 ocular surface damage.. This in-vitro study investigates how varying concentrations of 40 microplastics affect corneal cell viability and cytokine expression. Incubation:Article Title: CD200 + fibroblasts prime the immunoregulatory function of granulocytic myeloid-derived suppressor cells in inflammatory arthritis. Article Snippet: .. Secondary antibodies were stained for 1 h at room temperature followed by incubation with Article Title: Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice Article Snippet: For quantitative analysis of trans-synaptic tracing, 40-μm coronal sections of the whole brain were collected using cryostat (model #CM1860; Leica). .. Free-floating slices were incubated at room temperature with gentle agitation as follows: 2 h in blocking solution (5% heat-inactivated goat serum, 0.4% Triton X-100 in PBS); overnight in primary antibody diluted 1:1000 in blocking solution (mouse anti-GFP, GFP-1010, Aves Labs; or anti-RFP, 5f8, Chromotek); 2–3 h in secondary antibody diluted 1:500 (donkey anti-chicken-IgY Alexa488 or goat anti-rat IgG Cy3, Jackson ImmunoResearch); and 15 min in 2.5 μg/mL of Article Title: Donor-intrinsic regenerative potential outweighs histocompatibility in iPSC-derived skeletal muscle engraftment in nonhuman primates. Article Snippet: Cultured cells were washed with PBS and fixed with 4% paraformaldehyde (Millipore Sigma) for 15 min. .. Cells were then permeabilized with 0.3% Triton X-100 (Millipore Sigma) in PBS for 15 min at room temperature, blocked with 3% Bovine Serum Albumin (BSA) in PBS for 45 min, and incubated with primary antibodies overnight at 4 ◦ C. Following washing, cells were incubated with fluorophore-conjugated secondary antibodies containing Gentle:Article Title: Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice Article Snippet: For quantitative analysis of trans-synaptic tracing, 40-μm coronal sections of the whole brain were collected using cryostat (model #CM1860; Leica). .. Free-floating slices were incubated at room temperature with gentle agitation as follows: 2 h in blocking solution (5% heat-inactivated goat serum, 0.4% Triton X-100 in PBS); overnight in primary antibody diluted 1:1000 in blocking solution (mouse anti-GFP, GFP-1010, Aves Labs; or anti-RFP, 5f8, Chromotek); 2–3 h in secondary antibody diluted 1:500 (donkey anti-chicken-IgY Alexa488 or goat anti-rat IgG Cy3, Jackson ImmunoResearch); and 15 min in 2.5 μg/mL of Blocking Assay:Article Title: Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice Article Snippet: For quantitative analysis of trans-synaptic tracing, 40-μm coronal sections of the whole brain were collected using cryostat (model #CM1860; Leica). .. Free-floating slices were incubated at room temperature with gentle agitation as follows: 2 h in blocking solution (5% heat-inactivated goat serum, 0.4% Triton X-100 in PBS); overnight in primary antibody diluted 1:1000 in blocking solution (mouse anti-GFP, GFP-1010, Aves Labs; or anti-RFP, 5f8, Chromotek); 2–3 h in secondary antibody diluted 1:500 (donkey anti-chicken-IgY Alexa488 or goat anti-rat IgG Cy3, Jackson ImmunoResearch); and 15 min in 2.5 μg/mL of FACS:Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody Article Snippet: Cells were surface-stained with fluorescent dye-conjugated antibodies anti-human CD4 (clone RPA-T4, BioLegend, Cat.-No. 300532), CD8 (clone RPa-T8, BioLegend, Cat.-No. 3010441), CD62L (clone DREG-56, BioLegend, Cat.-No. 304834), CD127 (clone 019D5, BioLegend, Cat.-No. A019D5), CD134 (clone Ber-ACT35, BioLegend, Cat.-No. 350008), CD137 (clone 4B4-1, BioLegend, Cat.-No. 309814), GITR (clone 621, BioLegend, Cat.-No. 3311608) and CD25 (clone M-A251, BioLegend, Cat.-No. 356112) for 20 min at 4° C. in FACS buffer. .. Then, they were washed once with FACS buffer before being resuspended in 85 μL/well FACS buffer containing containing 0.2 μg/mL Software:Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody Article Snippet: Cells were surface-stained with fluorescent dye-conjugated antibodies anti-human CD4 (clone RPA-T4, BioLegend, Cat.-No. 300532), CD8 (clone RPa-T8, BioLegend, Cat.-No. 3010441), CD62L (clone DREG-56, BioLegend, Cat.-No. 304834), CD127 (clone 019D5, BioLegend, Cat.-No. A019D5), CD134 (clone Ber-ACT35, BioLegend, Cat.-No. 350008), CD137 (clone 4B4-1, BioLegend, Cat.-No. 309814), GITR (clone 621, BioLegend, Cat.-No. 3311608) and CD25 (clone M-A251, BioLegend, Cat.-No. 356112) for 20 min at 4° C. in FACS buffer. .. Then, they were washed once with FACS buffer before being resuspended in 85 μL/well FACS buffer containing containing 0.2 μg/mL |
