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4 6 diamidino 2 phenylindole dapi staining solution  (Beyotime)


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    Structured Review

    Beyotime 4 6 diamidino 2 phenylindole dapi staining solution
    4 6 Diamidino 2 Phenylindole Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+solution+c1002/DAPI/pmc12929097-49-0-7
    Average 99 stars, based on 32584 article reviews
    4 6 diamidino 2 phenylindole dapi staining solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination
    Article Snippet: .. Cell staining and viability assays were performed using a LIVE/DEAD kit (calcein AM/propidium iodide), an annexin V–FITC/PI apoptosis detection kit, MTT reagent, Alexa Fluor 488–conjugated secondary antibody, and DAPI (Beyotime Biotechnology, Shanghai, China). .. The JC-1 MitoMP Detection Kit was obtained from Dojindo Molecular Technologies Inc. (Kumamoto, Japan).

    Article Title: CD52+ cells are associated with faster short-term neutrophil recovery in mobilized hematopoietic stem cell transplant.
    Article Snippet: .. Cells were stained with CD52 primary antibody (Bioss, #bs-34117R) at 4 oC for overnight, and subsequently incubated for 2 hours at 37 oC with the Alexa Flur 488- conjugated anti-rabbit secondary antibody (Invitrogen, #A21206) and DAPI (Beyotime Biotechnology, #P0131) were used for depiction of the nuclei. .. Image acquisition was performed using either a confocal ultra-spectral microscope (Zeiss, LSM900).

    Article Title: DEAD-box RNA helicase 21 interacts with the nucleocapsid protein and inhibits porcine deltacoronavirus infection through upregulation of interferon responses.
    Article Snippet: The cells were then incubated with primary antibodies for 1 h, followed by washing with PBS and incubation with Alexa Fluor 488- and 594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 h at 37°C in the dark. .. Afterward, the cells were stained with DAPI (Cat. No. C1005, Beyotime), mounted with glycerin, and observed under a confocal microscope (Olympus). ..

    MTT Assay:

    Article Title: Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination
    Article Snippet: .. Cell staining and viability assays were performed using a LIVE/DEAD kit (calcein AM/propidium iodide), an annexin V–FITC/PI apoptosis detection kit, MTT reagent, Alexa Fluor 488–conjugated secondary antibody, and DAPI (Beyotime Biotechnology, Shanghai, China). .. The JC-1 MitoMP Detection Kit was obtained from Dojindo Molecular Technologies Inc. (Kumamoto, Japan).

    Incubation:

    Article Title: CD52+ cells are associated with faster short-term neutrophil recovery in mobilized hematopoietic stem cell transplant.
    Article Snippet: .. Cells were stained with CD52 primary antibody (Bioss, #bs-34117R) at 4 oC for overnight, and subsequently incubated for 2 hours at 37 oC with the Alexa Flur 488- conjugated anti-rabbit secondary antibody (Invitrogen, #A21206) and DAPI (Beyotime Biotechnology, #P0131) were used for depiction of the nuclei. .. Image acquisition was performed using either a confocal ultra-spectral microscope (Zeiss, LSM900).

    Article Title: Glucocorticoid-induced METTL3 promotes SONFH by enhancing MEG3 m6A methylation and subsequent ABL2 upregulation.
    Article Snippet: Background: Steroid-induced osteonecrosis of the femoral head (SONFH) is a refractory orthopedic disease.. While N6-methyladenosine (m6A) modification is implicated in bone metabolism, its specific role and mechanism in SONFH pathogenesis require further elucidation.. Methods: Bioinformatics analysis identified key m6A-related genes.

    TUNEL Assay:

    Article Title: Glucocorticoid-induced METTL3 promotes SONFH by enhancing MEG3 m6A methylation and subsequent ABL2 upregulation.
    Article Snippet: Background: Steroid-induced osteonecrosis of the femoral head (SONFH) is a refractory orthopedic disease.. While N6-methyladenosine (m6A) modification is implicated in bone metabolism, its specific role and mechanism in SONFH pathogenesis require further elucidation.. Methods: Bioinformatics analysis identified key m6A-related genes.

    Confocal:

    Article Title: DEAD-box RNA helicase 21 interacts with the nucleocapsid protein and inhibits porcine deltacoronavirus infection through upregulation of interferon responses.
    Article Snippet: The cells were then incubated with primary antibodies for 1 h, followed by washing with PBS and incubation with Alexa Fluor 488- and 594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 h at 37°C in the dark. .. Afterward, the cells were stained with DAPI (Cat. No. C1005, Beyotime), mounted with glycerin, and observed under a confocal microscope (Olympus). ..

    Microscopy:

    Article Title: DEAD-box RNA helicase 21 interacts with the nucleocapsid protein and inhibits porcine deltacoronavirus infection through upregulation of interferon responses.
    Article Snippet: The cells were then incubated with primary antibodies for 1 h, followed by washing with PBS and incubation with Alexa Fluor 488- and 594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 h at 37°C in the dark. .. Afterward, the cells were stained with DAPI (Cat. No. C1005, Beyotime), mounted with glycerin, and observed under a confocal microscope (Olympus). ..



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    Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence <t>(DAPI)</t> represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.
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    Beyotime 4 6 diamidino 2 phenylindole dapi nuclear staining solution
    Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence <t>(DAPI)</t> represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.
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    Image Search Results


    Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence (DAPI) represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.

    Journal: Journal of Advanced Research

    Article Title: Increased Rab1a accelerates osteoarthritis by inhibiting autophagy via activation of the mTORC1-S6K pathway

    doi: 10.1016/j.jare.2024.11.009

    Figure Lengend Snippet: Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence (DAPI) represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.

    Article Snippet: Subsequently, The TUNEL reaction mixture was applied at room temperature for 1 h. This was followed by coloring the nuclei with DAPI Staining Solution(Beyotime, China).

    Techniques: Transfection, Control, Immunohistochemical staining, TUNEL Assay, Staining, Fluorescence, End Labeling