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dapi staining solution  (Beyotime)


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    Structured Review

    Beyotime dapi staining solution
    Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2090 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+solution+c1002/DAPI+Staining+Solution/pm37153902-53-0-8
    Average 99 stars, based on 2090 article reviews
    dapi staining solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Labeling:

    Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos
    Article Snippet: .. Upon completion of all labeling procedures, DAPI staining solution (C1005, Beyotime, Shanghai, China) was added for 5 min of nuclear staining in the dark, and residual DAPI was washed off with PBS. .. Slides were treated with tissue autofluorescence quencher for 5 min in darkness and rinsed under running tap water for 10 min, then mounted with anti-fluorescence quenching mounting medium (P0126, Beyotime, Shanghai, China).

    Staining:

    Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos
    Article Snippet: .. Upon completion of all labeling procedures, DAPI staining solution (C1005, Beyotime, Shanghai, China) was added for 5 min of nuclear staining in the dark, and residual DAPI was washed off with PBS. .. Slides were treated with tissue autofluorescence quencher for 5 min in darkness and rinsed under running tap water for 10 min, then mounted with anti-fluorescence quenching mounting medium (P0126, Beyotime, Shanghai, China).

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury
    Article Snippet: .. The sections were rinsed with PBS for another 3 cycles, 5 min per wash. 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Cat. No.: C1005; Beyotime Jo urn al Pr e-p roo f 7 / 40 Biotechnology) was added dropwise and incubated at room temperature in the dark for 5 min for nuclear counterstaining. ..

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Article Title: Hierarchically sequential targeting nanoplatform suppresses NETosis-promoted angiogenesis for corneal neovascularization therapy.
    Article Snippet: The Phorbol 12-myristate 13-acetate (PMA, S1819) was purchased from Beyotime (Shanghai, China). .. The 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride Jo ur na l P re -p ro of (DAPI, C1005) Staining Solution was purchased from Beyotime (Shanghai, China). .. The clamidine hydrochloride was purchased from Sparkjade (SJ-MX0367A, Shandong, China).

    Article Title: Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding
    Article Snippet: Vector TrueVIEW kit , Vector laboratories , SP-8400. .. DAPI Staining Solution , Beyotime , C1006. .. mTeSR1 medium , STEMCELL , 85850.

    Incubation:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury
    Article Snippet: .. The sections were rinsed with PBS for another 3 cycles, 5 min per wash. 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Cat. No.: C1005; Beyotime Jo urn al Pr e-p roo f 7 / 40 Biotechnology) was added dropwise and incubated at room temperature in the dark for 5 min for nuclear counterstaining. ..

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Glass:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Inverted Microscopy:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Fluorescence:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    other:

    Article Title: Lacticaseibacillus rhamnosus GG enhances gefitinib efficacy and reduces treatment-related toxicity via propionyl-L-carnitine regulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Propionyl-L-carnitine (PLC) Aladdin Biochemical Technology, Shanghai, China CAS: 20064-19-1 Acetonitrile Xilong Scientific Co., Ltd., China CAS: 75-05-8 Formic acid Xilong Scientific Co., Ltd., China CAS: 64-18-6 Methotrexate Yuanye Bio-Technology, Shanghai, China CAS: 59-05-2 Luciferin sodium salt Yuanye Bio-Technology, Shanghai, China Cat#: B25437 Sodium carboxymethylcellulose (CMC-Na) Solarbio Life Sciences, Beijing, China CAS: 9004-32-4 Universal Tissue Fixative (Neutral) Servicebio, Wuhan, China Cat#: G1101 Trypsin Gibco Cat#: 25200-056 Fetal bovine serum Gibco Cat#: A5669701 Penicillin-streptomycin Gibco Cat#: 15140122 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#: 11965092 RPMI 1640 medium Gibco Cat#: C11875500BT BSA blocking solution Thermo Fisher Scientific Cat#: 37525 RIPA lysis buffer Thermo Fisher Scientific Cat#: 89901 RIPA buffer Beyotime Biotechnology Cat#: P0013B PMSF Beyotime Biotechnology Cat#: ST507 Protease/phosphatase inhibitor cocktail Beyotime Biotechnology Cat#: P1045 0.1% Triton X-100 Beyotime Biotechnology Cat#: P0096 DAPI staining solution Beyotime Biotechnology Cat#: C1006 Anhydrous Sodium Sulfate Xilong Scientific Co., Ltd., China CAS: 7757-82-6 Sodium Chloride (NaCl) Xilong Scientific Co., Ltd., China CAS: 7647-14-5 Dilute Sulfuric Acid (H2SO4) Xilong Scientific Co., Ltd., China CAS: 7664-93-9 n-Hexane Xilong Scientific Co., Ltd., China CAS: 110-54-3 Collagenase Type IV Sigma-Aldrich Cat#: C4-28 DNase I Beyotime Biotechnology Cat#: D7073 Critical commercial assays CCK-8 Kit MedChemExpress Cat#: HY-K0301 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat#: C0009S Annexin V-FITC/PI Apoptosis Detection Kit Huiyucheng Biotechnology, Wuhan, China Cat#: HYC2019 BCA Protein Assay Kit Thermo Fisher Scientific Cat#: 23225 TUNEL Assay Kit Beyotime Biotechnology Cat#: C1098 DAB Substrate Kit Beyotime Biotechnology Cat# P0202 Pierce ECL Western Blotting Substrate Thermo Fisher Scientific Cat#: 32106 CEA ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9M0916 CYFRA21-1 ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY926701a GLUT1 ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9NM11861 LDHA ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9NM1898 Mitochondrial Complex I Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737601a Mitochondrial Complex II Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737701a Mitochondrial Complex III Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737801a Mitochondrial Complex IV Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737901a Mitochondrial Complex V Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737011a Propionyl-L-carnitine (PLC) ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY972791a Mouse ROS ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XYM9437001 Mouse IKKα ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9438821a (Continued on next page) 18 Cell Reports 45, 118068, October 27, 2026



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    Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence <t>(DAPI)</t> represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.
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    Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence <t>(DAPI)</t> represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.
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    Image Search Results


    Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence (DAPI) represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.

    Journal: Journal of Advanced Research

    Article Title: Increased Rab1a accelerates osteoarthritis by inhibiting autophagy via activation of the mTORC1-S6K pathway

    doi: 10.1016/j.jare.2024.11.009

    Figure Lengend Snippet: Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence (DAPI) represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.

    Article Snippet: Subsequently, The TUNEL reaction mixture was applied at room temperature for 1 h. This was followed by coloring the nuclei with DAPI Staining Solution(Beyotime, China).

    Techniques: Transfection, Control, Immunohistochemical staining, TUNEL Assay, Staining, Fluorescence, End Labeling