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dapi  (Miltenyi Biotec)


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Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
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    Structured Review

    Miltenyi Biotec dapi
    Dapi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/DAPI+Staining+Solution/pm42811304-68-17-18
    Average 97 stars, based on 93 article reviews
    dapi - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Staining:

    Article Title: Receptor-guided AAV Tropism Engineering via MATCH
    Article Snippet: After 10 minutes, cells were stained with APC anti-human CD3 Antibody (BioLegend, 300412) and FITC anti-human CD25 Antibody (BioLegend, 302603) for 30 minutes on ice. .. Cells were washed once with 1✕ PBS with 2% FBS, pH 7.4, and finally resuspended in 1✕ PBS with 2% FBS and 0.1 μg/mL DAPI Staining Solution (Miltenyi Biotec, 130-111-570), pH 7.4 and analyzed on the BD LSRFortessaTM Cell Analyzer. ..

    Article Title: Receptor-guided AAV Tropism Engineering via MATCH
    Article Snippet: .. For general transduction experiments, 48 hours after AAV transduction, cells were centrifuged and resuspended in 1✕ PBS with 2% FBS and 0.1 μg/mL DAPI Staining Solution (Miltenyi Biotec, 130-111-570), pH 7.4 at 1 ✕ 106 cells/mL and analyzed on the BD LSRFortessaTM Cell Analyzer, gating for live cells and mCherry or GFP fluorescence. ..

    Article Title: Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1.
    Article Snippet: Analytical flow cytometry was performed on an Attune NxT flow cytometer (Thermo Fisher Scientific) equipped with an automated plate reader. .. Cells were prepared by single-cell dissociation using TrypLE, then resuspended in 1 × DPBS supplemented with 2mM EDTA and DAPI staining solution (Miltenyi Biotec) for live/dead discrimination. ..

    Article Title: An Integrin β6-targeted antibody-drug conjugate optimized for intravesical delivery to treat non-muscle invasive bladder cancer
    Article Snippet: PDOs and cells were dissociated with TrypLE (ThermoFisher, 12605010) for 5-10 min at 37°C, then distributed into 96-well V-bottom plates (2 x 105 cells/well). .. Cells were stained with h2A2 primary antibody (1 hour, 4°C), anti-IgG-FITC (45 minutes, 4°C) and DAPI (Miltenyi Biotec, 130-111-570) (5 minutes). .. Samples were acquired within 4 hours on a MACSQuant Analyzer 10 (Miltenyi Biotec, RRID:SCR_020268) or Attune NxT flow cytometer and analyzed using FlowJo v10.8.

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: For the detection of CARs or nsCARs on T-cells in the three fractions (pre-MACS, flow-through, and post-MACS), staining was performed using FITC- or PE-conjugated CD34 antibodies (clone QBEND/10, Thermo Fisher Scientific; FITC: MA1-10204, PE: MA1-10205) and APC- or PE-Vio770-conjugated CD271 antibodies (clone ME20.4, Miltenyi Biotec; APC: 130-113-418, PE-Vio770: 130-133-422). .. After staining and washing, cells were resuspended in MACS buffer containing either PI (Thermo Scientific; P1304MP) or DAPI (Miltenyi Biotec; 130-111-570) for live/dead cell discrimination. ..

    Transduction:

    Article Title: Receptor-guided AAV Tropism Engineering via MATCH
    Article Snippet: .. For general transduction experiments, 48 hours after AAV transduction, cells were centrifuged and resuspended in 1✕ PBS with 2% FBS and 0.1 μg/mL DAPI Staining Solution (Miltenyi Biotec, 130-111-570), pH 7.4 at 1 ✕ 106 cells/mL and analyzed on the BD LSRFortessaTM Cell Analyzer, gating for live cells and mCherry or GFP fluorescence. ..

    Bioprocessing:

    Article Title: Receptor-guided AAV Tropism Engineering via MATCH
    Article Snippet: .. For general transduction experiments, 48 hours after AAV transduction, cells were centrifuged and resuspended in 1✕ PBS with 2% FBS and 0.1 μg/mL DAPI Staining Solution (Miltenyi Biotec, 130-111-570), pH 7.4 at 1 ✕ 106 cells/mL and analyzed on the BD LSRFortessaTM Cell Analyzer, gating for live cells and mCherry or GFP fluorescence. ..

    Fluorescence:

    Article Title: Receptor-guided AAV Tropism Engineering via MATCH
    Article Snippet: .. For general transduction experiments, 48 hours after AAV transduction, cells were centrifuged and resuspended in 1✕ PBS with 2% FBS and 0.1 μg/mL DAPI Staining Solution (Miltenyi Biotec, 130-111-570), pH 7.4 at 1 ✕ 106 cells/mL and analyzed on the BD LSRFortessaTM Cell Analyzer, gating for live cells and mCherry or GFP fluorescence. ..

    Single Cell:

    Article Title: Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1.
    Article Snippet: Analytical flow cytometry was performed on an Attune NxT flow cytometer (Thermo Fisher Scientific) equipped with an automated plate reader. .. Cells were prepared by single-cell dissociation using TrypLE, then resuspended in 1 × DPBS supplemented with 2mM EDTA and DAPI staining solution (Miltenyi Biotec) for live/dead discrimination. ..

    Incubation:

    Article Title: Hyaluronic acid and tissue mechanics orchestrate mammalian digit tip regeneration.
    Article Snippet: .. Then, cells were incubated with ready- to- use DAPI (Miltenyi Biotec, 130- 111- 570) for 5 min in 2% FBS and 2.5 mM EDTA in PBS. ..

    Magnetic Cell Separation:

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: For the detection of CARs or nsCARs on T-cells in the three fractions (pre-MACS, flow-through, and post-MACS), staining was performed using FITC- or PE-conjugated CD34 antibodies (clone QBEND/10, Thermo Fisher Scientific; FITC: MA1-10204, PE: MA1-10205) and APC- or PE-Vio770-conjugated CD271 antibodies (clone ME20.4, Miltenyi Biotec; APC: 130-113-418, PE-Vio770: 130-133-422). .. After staining and washing, cells were resuspended in MACS buffer containing either PI (Thermo Scientific; P1304MP) or DAPI (Miltenyi Biotec; 130-111-570) for live/dead cell discrimination. ..



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