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dapi  (Dojindo Labs)


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    Structured Review

    Dojindo Labs dapi
    Dapi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/Cellstain+DAPI+solution/pmc13636658-94-53-54
    Average 96 stars, based on 489 article reviews
    dapi - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: A CD4 + T cell-fibroblast crosstalk exacerbates autoimmunity in a mouse model of primary Sjögren disease.
    Article Snippet: To evaluate nuclear translocation of NF-κB p65, fibroblasts (1×105 cells/well) from WT- or Tnfrsf8-deficient pSjD model mouse SG were first AR TI CL E IN P RE SS cultured in DMEM containing 10% FBS on collagen coated-culture cover glass (Matsunami Glass Ind., Ltd., Cat# CS1163) placed in a 24-well plate for one day.

    Article Title: Inter-organ metabolic feedback via BCAA catabolism regulates glucagon-like hormone secretion in Drosophila.
    Article Snippet: Primary and secondary antibodies used were rabbit polyclonal anti-phospho-S6K (1:500, #9209, Cell Signaling Technology), rabbit monoclonal anti-phospho-Akt (1:500, #4060, Cell Signaling Technology), rabbit monoclonal anti-Akt (1:500, #4691, Cell Signaling Technology), goat polyclonal anti-GAPDH (1:500, IMG-3073, Imgenex), HRP-linked anti-rabbit IgG (1:2,000, #7074, Cell Signaling Technology), and HRP-linked anti-goat IgG (1:2,000, ab97110, Abcam).

    Staining:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Incubation:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Blocking Assay:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Cell-Signaling:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.



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