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1 mg/ml 4,6-diamidino-2-phenylindole hydrochloride (dapi) solution  (Millipore)


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    Millipore 1 mg/ml 4,6-diamidino-2-phenylindole hydrochloride (dapi) solution
    1 Mg/Ml 4,6 Diamidino 2 Phenylindole Hydrochloride (Dapi) Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution+1+mg/dapi/pmc04499382-41-8-12
    Average 90 stars, based on 1 article reviews
    1 mg/ml 4,6-diamidino-2-phenylindole hydrochloride (dapi) solution - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells are incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections are mounted on slides with FluorSave reagent (Millipore). .. Images are taken using an Olympus IX83 microscope (Olympus).

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells were incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections were mounted on slides with FluorSave reagent (Millipore). .. Images were taken using an Olympus IX83 microscope (Olympus).

    Marker:

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells are incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections are mounted on slides with FluorSave reagent (Millipore). .. Images are taken using an Olympus IX83 microscope (Olympus).

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells were incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections were mounted on slides with FluorSave reagent (Millipore). .. Images were taken using an Olympus IX83 microscope (Olympus).

    Staining:

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells are incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections are mounted on slides with FluorSave reagent (Millipore). .. Images are taken using an Olympus IX83 microscope (Olympus).

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells were incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections were mounted on slides with FluorSave reagent (Millipore). .. Images were taken using an Olympus IX83 microscope (Olympus).

    Fluorsave:

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells are incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections are mounted on slides with FluorSave reagent (Millipore). .. Images are taken using an Olympus IX83 microscope (Olympus).

    Article Title: Fusion protein comprising IL13
    Article Snippet: .. Cells were incubated with rabbit anti-β3 tubulin (Anti-beta III Tubulin antibody-Neuronal Marker, ab18207, 1:1500, Abcam, Cambridge, UK) and NeuN (Anti-NeuN Antibody clone A60, MAB377, 1:500) Sigma Aldrich (Merck), Darmstadt, Germany) overnight at 4° C., followed by washes and incubation with AF488-conjugated donkey anti-rabbit and 568-conjugated donkey anti-mouse secondary antibodies (Thermofisher, 1:500) followed by DAPI (1:5000, Sigma) staining before sections were mounted on slides with FluorSave reagent (Millipore). .. Images were taken using an Olympus IX83 microscope (Olympus).

    other:

    Article Title: Regulatory T Cells Boost Efficacy of Post-Infarction Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cell Transplants.
    Article Snippet: The number of capillaries were counted and expressed as the number of capillaries per square millimetre (mm2) using a staining Griffonia Bandeiraea Simplicifolia Lectin I (20 mg/mL, Vector, CA, USA) conjugated with rhodamine, in conjunction with the nuclear marker 4′,6-diamidino-2-phenylindole (1 mg/mL, DAPI, Calbiochem, France).

    Article Title: Supplemental Information DOT1L interaction partner AF10 controls patterning of H3K79 methyla- tion and RNA polymerase II to maintain cell identity
    Article Snippet: Coverslips were washed once, incubated with 4′,6-Diamidino-2-phenylindole dihydrochloride (1:10,000; Millipore Sigma, D8417) in wash buffer, and then washed a second time before mounting with Aqua-Poly/Mount (Fisher Scientific, NC9439247).

    Article Title: Hypoxia-Induced Metabolic Reprogramming and Markings of Cell Fate in Concentric Arterial Hypertrophy
    Article Snippet: Residual red blood cells were lysed by Red Blood Cell Lysis Buffer (Sigma-Aldrich).

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998

    Article Title: Unique recognition, phagocytosis, and intracellular survival of Cryptococcus neoformans in a human immortalized microglia cell line
    Article Snippet: Coverslips were washed with PBS and a corresponding Alexa Fluor-conjugated goat secondary antibody (Invitrogen) was diluted to 1 μg/mL in PBS with 2 μg/mL of 4’6-diamidino-2-phenylindole (DAPI; Millipore Sigma) and added to cells for 1 hour at RT.



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    Image Search Results


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    Article Snippet: Chemical compound, drug , DAPI solution (1 mg/mL) , Invitrogen , Cat#62248 , 1:1000.

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