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caspase 3 (Boster Bio)

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Boster Bio caspase 3

Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, Bcl-2, Bax, <t>caspase-3,</t> and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.

Caspase 3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cpp/pmc12841121-266-20-22?v=Boster+Bio
Average 93 stars, based on 94 article reviews

caspase 3 - by Bioz Stars, 2026-08

93/100 stars

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1) Product Images from "Altered Magnesium Environments Restrict Colorectal HT-29 Spheroid Growth by Disturbing Cellular Mg 2+ Homeostasis"

Article Title: Altered Magnesium Environments Restrict Colorectal HT-29 Spheroid Growth by Disturbing Cellular Mg 2+ Homeostasis

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms27020834

Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, Bcl-2, Bax, caspase-3, and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.

Figure Legend Snippet: Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, Bcl-2, Bax, caspase-3, and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.

Techniques Used: Western Blot, Derivative Assay, Cell Culture, Control, Membrane, Fluorescence

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caspase 3 (Boster Bio)

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1) Product Images from "Altered Magnesium Environments Restrict Colorectal HT-29 Spheroid Growth by Disturbing Cellular Mg 2+ Homeostasis"

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