Journal: eLife

Article Title: Synapse-specific opioid modulation of thalamo-cortico-striatal circuits

doi: 10.7554/eLife.45146

Figure Lengend Snippet:

Article Snippet: Chemical compound, drug , CPP , R and D Systems/Tocris , Cat# 0173 , .

Techniques: Virus, Plasmid Preparation, RNAscope, Multiplex Assay, Fluorescence, Software

Journal: Translational Stroke Research

Article Title: Remote Ischemic Preconditioning Exerts Neuroprotective Effects Via the PGC-1α/FNDC5/BDNF Pathway in Focal Brain Ischemia of Rats

doi: 10.1007/s12975-026-01422-z

Figure Lengend Snippet: Effects of RIpreC on the expression of Bcl-2 ( A , B , F , G ), Bax ( A , C , F , H ), caspase-3 ( A , D , F , J ), and TUNEL-positive neurons ( A , E ) in the ischemic brain. Bcl-2 immunoreactivity in the perilesional cortex and its protein level were significantly increased in the RIpreC group compared the IR group. By contrast, Bax immunoreactivity and its protein level were significantly reduced in the RIpreC group compared with the IR group. The Bax/Bcl-2 ratios were also significantly reduced in the RIpreC group compared with the IR group ( I ). Subsequently, the number of TUNEL-positive neurons (arrow) and the ratios of caspase-3 positive to NeuN-positive neuron in the perilesional cortex were significantly decreased in the RIpreC group compared with the IR group ( E , K , L ). Mean ± standard error. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bar = 50 μm ( A ) and 25 μm ( K ). ( n = 4–11 in each group)

Article Snippet: # p < 0.05 (Mann–Whitney U test), # p < 0.01 (Student’s t-test), Scale bar = 1 mm ( B ). ( n = 5–11 in each group) Co-localization of mouse anti-neuronal nuclei (NeuN) antibody (1:200; a marker of neurons; ab104224; Abcam plc, Cambridge, UK) with rabbit anti-caspase-3 antibody (1:200; 19677-1-AP; Proteintech, USA) immunoreactivities was examined using immunofluorescence staining.

Techniques: Expressing, TUNEL Assay

Journal: Translational Stroke Research

Article Title: Remote Ischemic Preconditioning Exerts Neuroprotective Effects Via the PGC-1α/FNDC5/BDNF Pathway in Focal Brain Ischemia of Rats

doi: 10.1007/s12975-026-01422-z

Figure Lengend Snippet: Effects of PGC-1αInhibition by SR-18,292. Inhibition of PGC-1α blunted the neuroprotective and neurorestorative effects of RIpreC ( A – F ). No changes in brain infarctions, neurological scores, and sensorimotor functions were observed in the RIpreC-treated SR-18,292 group compared with the IR group. Immunohistochemical analysis of the rectangular areas in A showed that immunoreactivities of PGC-1α, FNDC5, BDNF, and caspase-3 surrounding the lesion in the RIpreC-treated SR-18,292 group were similar to those in the IR group ( G – K ). Scale bar = 50 μm ( G ). ( n = 5–11 in each group)

Article Snippet: # p < 0.05 (Mann–Whitney U test), # p < 0.01 (Student’s t-test), Scale bar = 1 mm ( B ). ( n = 5–11 in each group) Co-localization of mouse anti-neuronal nuclei (NeuN) antibody (1:200; a marker of neurons; ab104224; Abcam plc, Cambridge, UK) with rabbit anti-caspase-3 antibody (1:200; 19677-1-AP; Proteintech, USA) immunoreactivities was examined using immunofluorescence staining.

Techniques: Inhibition, Immunohistochemical staining

Journal: International Journal of Molecular Sciences

Article Title: Altered Magnesium Environments Restrict Colorectal HT-29 Spheroid Growth by Disturbing Cellular Mg 2+ Homeostasis

doi: 10.3390/ijms27020834

Figure Lengend Snippet: Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, Bcl-2, Bax, caspase-3, and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.

Article Snippet: Membranes were probed with specific primary antibodies against p53 (PB9008; Bosterbio, Pleasanton, CA, USA), BCL-2 (A00040; Bosterbio), Bax (A00183; Bosterbio), caspase-3 (PB9188; Bosterbio), cleaved caspase-3 (ab32042; Abcam), pS556 ULK1 (ab203207; Abcam), p62/SQSTM1 (ab109012; Abcam), TRPM6 (0ST00108W; Thermo Fisher Scientific Inc.), TRPM7 (ab729; Abcam), MagT1 (ab244490; Abcam), CNNM4 (ab191207; Abcam), Mrs2 (ab246915; Abcam), β-actin (ab6276; Abcam), and Na + /K + -ATpase (ab76020; Abcam).

Techniques: Western Blot, Derivative Assay, Cell Culture, Control, Membrane, Fluorescence

Journal: Metabolites

Article Title: Improvements in Insulin Resistance and Glucose Metabolism Related to Breastfeeding Are Not Mediated by Subclinical Inflammation

doi: 10.3390/metabo14110608

Figure Lengend Snippet: Participants’ data throughout pre-gestation, third trimester, and postpartum periods, according to breastfeeding status.

Article Snippet: E-selectin (Human SELE Elabscience-E-EL-H0876; Houston, TX, USA), zonulin (Human Zonulin Elabscience-E-EL-H5560; Houston, TX, USA), adiponectin (Human ADP/Acrp30 Elabscience-E-EL-H6122; Houston, TX, USA), copeptin (Human CPP Elabscience-E-EL-H0851; Houston, TX, USA) and lipopolysaccharides (LPS—Human LBP Elabscience-E-EL-H6108; Houston, TX, USA) were measured using enzyme-linked immunosorbent assay (ELISA Kit, Elabscience Kit; Houston, TX, USA), while branched-chain amino acids (BCAAs) were assessed using High-Performance Liquid Chromatography (MBS169297, HPLC, My Biosource; San Diego, CA, USA).

Techniques: Activity Assay

Journal: Cancer Cell International

Article Title: Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

doi: 10.1186/s12935-025-04089-y

Figure Lengend Snippet: UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of caspase-9, and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars

Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: UNG, P70 S6K, p-P70 S6K(S424) (1:1000, Bioworld), β-actin (1:1000, Abcam), cleaved caspase-3, AMPKα1/AMPKα2, p-AMPKα1(Thr183)/AMPKα2(Thr172) (1:1000, Beyotime), BAX, BCL2, AKT1/2/3, mTOR, and p-mTOR (Ser2448) (1:1000; BOSTER), cleaved caspase-9, p-AKT(Ser473), ERK1/2, and p-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) (1:1000; ZEN BIO), and GAPDH (1:1000; Proteintech).

Techniques: Protein-Protein interactions, Western Blot, Knockdown