Journal: eLife

Article Title: Synapse-specific opioid modulation of thalamo-cortico-striatal circuits

doi: 10.7554/eLife.45146

Figure Lengend Snippet:

Article Snippet: Chemical compound, drug , CPP , R and D Systems/Tocris , Cat# 0173 , .

Techniques: Virus, Plasmid Preparation, RNAscope, Multiplex Assay, Fluorescence, Software

Journal: International Journal of Molecular Sciences

Article Title: Altered Magnesium Environments Restrict Colorectal HT-29 Spheroid Growth by Disturbing Cellular Mg 2+ Homeostasis

doi: 10.3390/ijms27020834

Figure Lengend Snippet: Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, Bcl-2, Bax, caspase-3, and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.

Article Snippet: Membranes were probed with specific primary antibodies against p53 (PB9008; Bosterbio, Pleasanton, CA, USA), BCL-2 (A00040; Bosterbio), Bax (A00183; Bosterbio), caspase-3 (PB9188; Bosterbio), cleaved caspase-3 (ab32042; Abcam), pS556 ULK1 (ab203207; Abcam), p62/SQSTM1 (ab109012; Abcam), TRPM6 (0ST00108W; Thermo Fisher Scientific Inc.), TRPM7 (ab729; Abcam), MagT1 (ab244490; Abcam), CNNM4 (ab191207; Abcam), Mrs2 (ab246915; Abcam), β-actin (ab6276; Abcam), and Na + /K + -ATpase (ab76020; Abcam).

Techniques: Western Blot, Derivative Assay, Cell Culture, Control, Membrane, Fluorescence

Journal: Metabolites

Article Title: Improvements in Insulin Resistance and Glucose Metabolism Related to Breastfeeding Are Not Mediated by Subclinical Inflammation

doi: 10.3390/metabo14110608

Figure Lengend Snippet: Participants’ data throughout pre-gestation, third trimester, and postpartum periods, according to breastfeeding status.

Article Snippet: E-selectin (Human SELE Elabscience-E-EL-H0876; Houston, TX, USA), zonulin (Human Zonulin Elabscience-E-EL-H5560; Houston, TX, USA), adiponectin (Human ADP/Acrp30 Elabscience-E-EL-H6122; Houston, TX, USA), copeptin (Human CPP Elabscience-E-EL-H0851; Houston, TX, USA) and lipopolysaccharides (LPS—Human LBP Elabscience-E-EL-H6108; Houston, TX, USA) were measured using enzyme-linked immunosorbent assay (ELISA Kit, Elabscience Kit; Houston, TX, USA), while branched-chain amino acids (BCAAs) were assessed using High-Performance Liquid Chromatography (MBS169297, HPLC, My Biosource; San Diego, CA, USA).

Techniques: Activity Assay

Journal: Cancer Cell International

Article Title: Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

doi: 10.1186/s12935-025-04089-y

Figure Lengend Snippet: UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of caspase-9, and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars

Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: UNG, P70 S6K, p-P70 S6K(S424) (1:1000, Bioworld), β-actin (1:1000, Abcam), cleaved caspase-3, AMPKα1/AMPKα2, p-AMPKα1(Thr183)/AMPKα2(Thr172) (1:1000, Beyotime), BAX, BCL2, AKT1/2/3, mTOR, and p-mTOR (Ser2448) (1:1000; BOSTER), cleaved caspase-9, p-AKT(Ser473), ERK1/2, and p-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) (1:1000; ZEN BIO), and GAPDH (1:1000; Proteintech).

Techniques: Protein-Protein interactions, Western Blot, Knockdown