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ls400 confocal microarray scanner  (Tecan Systems)


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    Structured Review

    Tecan Systems ls400 confocal microarray scanner
    Ls400 Confocal Microarray Scanner, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/confocal+microarray+scanner/ls400+microarray+scanner/pm40664182-99-5-9
    Average 90 stars, based on 1 article reviews
    ls400 confocal microarray scanner - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Autoantibody screening of plasma and peritoneal fluid of patients with endometriosis.
    Article Snippet: 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Warsaw, Poland OVIklinika Infertility Center, Warsaw, Poland Women’s Health Research Institute, Calisia University, Kalisz, Poland Department of Obstetrics, Gynecology and Gynecological Oncology, Medical University of Warsaw, Warsaw, Poland Clinic of Gynecology, Oncological Gynecology and Obstetrics, Municipal Polyclinical Hospital in Olsztyn, Olsztyn, Poland Angelius Provita Hospital, Katowice, Poland Center for Gynecology, Obstetrics and Infertility Treatment Pastelova, Poznan, Poland Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, Poznan, Poland Chair and Department of Laboratory Diagnostics, Poznan University of Medical Sciences, Poznan, Poland Department of Obstetrics and Gynecology, Institute of Mother and Child, Warsaw, Poland Department of Obstetrics and Gynecology, Central Clinical Hospital of the Ministry of Interior, Warsaw, Poland Collegium Medicum Jan Kochanowski University in Kielce, Kielce, Poland Clinic of Obstetrics and Gynecology, Provincial Combined Hospital in Kielce, Kielce, Poland Department of Gynecology and Obstetrics Medical, University of Lodz, Lodz, Poland Department of Surgical Gynecology and Oncology, Medical University of Lodz, Lodz, Poland Department of Fetal Medicine and Gynecology, Medical University of Lodz, Lodz, Poland Clinic of Obstetric and Gynecology in Przemysl, Przemysl, Poland University of Rzeszow, Rzeszow, Poland Department of Gynecology, Gynecology Oncology and Obstetrics, Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszow, Poland IPDx Diagnostics OY, Tallinn, Estonia Institute of Computer Science, University of Tartu, Tartu, Estonia Competence Centre on Health Technologies, Tartu, Estonia Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, Tartu, Estonia Division of Obstetrics and Gynaecology, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institute and Karolinska University Hospital, Stockholm, Sweden

    Article Title: Para-infectious brain injury in COVID-19 persists at follow-up despite attenuated cytokine and autoantibody responses.
    Article Snippet: Briefly, a protein arrayof antigens (based on the HuProtTM (version 4.0) platform) was used to measure bound IgM and IgG from sera, using secondary antibodies with different fluorescent labels detected by a Tecan LS400 scanner andGenePix Pro v4 software.

    Microarray:

    Article Title: Synthesis of trisaccharide antigens featuring colitose, abequose and fucose residues and assessment of antibody binding on antigen arrays.
    Article Snippet: The detection of breast milk IgA and antibody binding to LPS or trisaccharide antigens was done using Cy3 AffiniPureTM Goat Anti-Human IgA, α chain specific (109-165-011; Jackson ImmunoResearch) and Cy3 AffiniPureTM Goat Anti-Rabbit IgG (H + L) (111-165-003; Jackson ImmunoResearch), respectively. .. The slides were scanned using the LS400 confocal microarray scanner (Tecan, Männerdorf, Switzerland) with the ArrayPro software (Media 366 Cybernetics, Rockerville, USA) and Cy3 fluorescence was excited at 532 nm and detected at 590 nm. ..

    Article Title: Differential Recognition of Lipopolysaccharide O-Antigens by the Pattern Recognition Molecules MBL and Ficolins of the Complement System.
    Article Snippet: Detection was done using Mab anti-MBL Cy3 (0.005 μg/mL), Mab anti-Mficolin Cy3 (1.5 μg/mL), Mab anti-H-ficolin Cy3 (1 μg/mL) and Mab anti-L-ficolin Cy3 (1 μg/mL), diluted in washing buffer without CaCl2. .. Scanning was performed using the LS400 confocal microarray scanner (Tecan, Männedorf, Switzerland). ..

    Article Title: Inter-individual and inter-regional variability of breast milk antibody reactivity to bacterial lipopolysaccharides
    Article Snippet: .. The slides were scanned immediately after processing using an LS400 confocal microarray scanner (Tecan, Männedorf, Switzerland). ..

    Article Title: Inter-individual and inter-regional variability of breast milk antibody reactivity to bacterial lipopolysaccharides
    Article Snippet: .. The slides were scanned immediately after processing using an LS400 confocal microarray scanner (Tecan, Männedorf, Switzerland). ..

    Software:

    Article Title: Synthesis of trisaccharide antigens featuring colitose, abequose and fucose residues and assessment of antibody binding on antigen arrays.
    Article Snippet: The detection of breast milk IgA and antibody binding to LPS or trisaccharide antigens was done using Cy3 AffiniPureTM Goat Anti-Human IgA, α chain specific (109-165-011; Jackson ImmunoResearch) and Cy3 AffiniPureTM Goat Anti-Rabbit IgG (H + L) (111-165-003; Jackson ImmunoResearch), respectively. .. The slides were scanned using the LS400 confocal microarray scanner (Tecan, Männerdorf, Switzerland) with the ArrayPro software (Media 366 Cybernetics, Rockerville, USA) and Cy3 fluorescence was excited at 532 nm and detected at 590 nm. ..

    Fluorescence:

    Article Title: Synthesis of trisaccharide antigens featuring colitose, abequose and fucose residues and assessment of antibody binding on antigen arrays.
    Article Snippet: The detection of breast milk IgA and antibody binding to LPS or trisaccharide antigens was done using Cy3 AffiniPureTM Goat Anti-Human IgA, α chain specific (109-165-011; Jackson ImmunoResearch) and Cy3 AffiniPureTM Goat Anti-Rabbit IgG (H + L) (111-165-003; Jackson ImmunoResearch), respectively. .. The slides were scanned using the LS400 confocal microarray scanner (Tecan, Männerdorf, Switzerland) with the ArrayPro software (Media 366 Cybernetics, Rockerville, USA) and Cy3 fluorescence was excited at 532 nm and detected at 590 nm. ..

    Article Title: Nanobody engineering for SARS-CoV-2 neutralization and detection.
    Article Snippet: .. The Dylight 633-labeled nanobodies bound to the arrayed antigens were detected by fluorescence scanning through the clear bottom of the microplate with a Tecan LS400 confocal laser scanner, using a 633 nm laser for excitation and a 670/25 emission filter. ..

    Article Title: Nanobody engineering for SARS-CoV-2 neutralization and detection
    Article Snippet: .. The Dylight 633-labeled nanobodies bound to the arrayed antigens were detected by fluorescence scanning through the clear bottom of the microplate with a Tecan LS400 confocal laser scanner, using a 633 nm laser for excitation and a 670/25 emission filter. ..



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    Effects of physiological hypoxia (physioxia) on proliferation, spontaneous differentiation and cell cycle phase distribution of fetal mNSCs. (A) Representative bright-field images of E13.5 fetal mNSCs cultured for 6 or 13 days in 3% or 21% O 2 . Scale bar is 25 μm. (B) Representative images of mNSCs stained for Tuj1, Map2, GFAP, Nestin and Hoechst. A total of 10 5 cells were seeded on a coverslip and grown for three days under physioxic or normoxic conditions. Scale bar is 50 μm. (C) Comparison of a marker selection of midbrain NSC development (from left to right side) showed additional reduction of late neuronal markers such as Lmo3 or Sox6 . (D) Quantitative analysis of immunoreactivity of mNSCs grown in 3% or 21% O 2 for Tuj1, Map2, GFAP and Nestin normalised to the total number of cells (Hoechst + ) in percent. (E) Quantitative analysis of the fraction of mNSCs in the different cell cycle phases of mNSCs grown in 3 and 21% O 2 . Plots show the relative distribution of cells grown under 3% or 21% O 2 across the different cell cycle phases G0-G1, S or G2-M in percent. (F, G) Comparison of cell cycle markers (F) and senescence/quiescence markers (G) of mNSCs by <t>microarray</t> analysis. Heat-maps represent the fold change of the relative mRNA expression levels of mNSCs cultured under physioxia for 48 h or 13 d in comparison to those cultured under normoxia. Colour bar displays the colour contrast level of the heat-map with red and green indicating high and low expression levels for (C, F, G). *p<0.05, **p<0.01, ***p<0.001 in respect to 21% O 2 (unpaired two-sided t-test with Bonferroni correction).
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    Effects of physiological hypoxia (physioxia) on proliferation, spontaneous differentiation and cell cycle phase distribution of fetal mNSCs. (A) Representative bright-field images of E13.5 fetal mNSCs cultured for 6 or 13 days in 3% or 21% O 2 . Scale bar is 25 μm. (B) Representative images of mNSCs stained for Tuj1, Map2, GFAP, Nestin and Hoechst. A total of 10 5 cells were seeded on a coverslip and grown for three days under physioxic or normoxic conditions. Scale bar is 50 μm. (C) Comparison of a marker selection of midbrain NSC development (from left to right side) showed additional reduction of late neuronal markers such as Lmo3 or Sox6 . (D) Quantitative analysis of immunoreactivity of mNSCs grown in 3% or 21% O 2 for Tuj1, Map2, GFAP and Nestin normalised to the total number of cells (Hoechst + ) in percent. (E) Quantitative analysis of the fraction of mNSCs in the different cell cycle phases of mNSCs grown in 3 and 21% O 2 . Plots show the relative distribution of cells grown under 3% or 21% O 2 across the different cell cycle phases G0-G1, S or G2-M in percent. (F, G) Comparison of cell cycle markers (F) and senescence/quiescence markers (G) of mNSCs by <t>microarray</t> analysis. Heat-maps represent the fold change of the relative mRNA expression levels of mNSCs cultured under physioxia for 48 h or 13 d in comparison to those cultured under normoxia. Colour bar displays the colour contrast level of the heat-map with red and green indicating high and low expression levels for (C, F, G). *p<0.05, **p<0.01, ***p<0.001 in respect to 21% O 2 (unpaired two-sided t-test with Bonferroni correction).
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    Effects of physiological hypoxia (physioxia) on proliferation, spontaneous differentiation and cell cycle phase distribution of fetal mNSCs. (A) Representative bright-field images of E13.5 fetal mNSCs cultured for 6 or 13 days in 3% or 21% O 2 . Scale bar is 25 μm. (B) Representative images of mNSCs stained for Tuj1, Map2, GFAP, Nestin and Hoechst. A total of 10 5 cells were seeded on a coverslip and grown for three days under physioxic or normoxic conditions. Scale bar is 50 μm. (C) Comparison of a marker selection of midbrain NSC development (from left to right side) showed additional reduction of late neuronal markers such as Lmo3 or Sox6 . (D) Quantitative analysis of immunoreactivity of mNSCs grown in 3% or 21% O 2 for Tuj1, Map2, GFAP and Nestin normalised to the total number of cells (Hoechst + ) in percent. (E) Quantitative analysis of the fraction of mNSCs in the different cell cycle phases of mNSCs grown in 3 and 21% O 2 . Plots show the relative distribution of cells grown under 3% or 21% O 2 across the different cell cycle phases G0-G1, S or G2-M in percent. (F, G) Comparison of cell cycle markers (F) and senescence/quiescence markers (G) of mNSCs by <t>microarray</t> analysis. Heat-maps represent the fold change of the relative mRNA expression levels of mNSCs cultured under physioxia for 48 h or 13 d in comparison to those cultured under normoxia. Colour bar displays the colour contrast level of the heat-map with red and green indicating high and low expression levels for (C, F, G). *p<0.05, **p<0.01, ***p<0.001 in respect to 21% O 2 (unpaired two-sided t-test with Bonferroni correction).
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    Image Search Results


    Effects of physiological hypoxia (physioxia) on proliferation, spontaneous differentiation and cell cycle phase distribution of fetal mNSCs. (A) Representative bright-field images of E13.5 fetal mNSCs cultured for 6 or 13 days in 3% or 21% O 2 . Scale bar is 25 μm. (B) Representative images of mNSCs stained for Tuj1, Map2, GFAP, Nestin and Hoechst. A total of 10 5 cells were seeded on a coverslip and grown for three days under physioxic or normoxic conditions. Scale bar is 50 μm. (C) Comparison of a marker selection of midbrain NSC development (from left to right side) showed additional reduction of late neuronal markers such as Lmo3 or Sox6 . (D) Quantitative analysis of immunoreactivity of mNSCs grown in 3% or 21% O 2 for Tuj1, Map2, GFAP and Nestin normalised to the total number of cells (Hoechst + ) in percent. (E) Quantitative analysis of the fraction of mNSCs in the different cell cycle phases of mNSCs grown in 3 and 21% O 2 . Plots show the relative distribution of cells grown under 3% or 21% O 2 across the different cell cycle phases G0-G1, S or G2-M in percent. (F, G) Comparison of cell cycle markers (F) and senescence/quiescence markers (G) of mNSCs by microarray analysis. Heat-maps represent the fold change of the relative mRNA expression levels of mNSCs cultured under physioxia for 48 h or 13 d in comparison to those cultured under normoxia. Colour bar displays the colour contrast level of the heat-map with red and green indicating high and low expression levels for (C, F, G). *p<0.05, **p<0.01, ***p<0.001 in respect to 21% O 2 (unpaired two-sided t-test with Bonferroni correction).

    Journal: International Journal of Stem Cells

    Article Title: Notch Is Not Involved in Physioxia-Mediated Stem Cell Maintenance in Midbrain Neural Stem Cells

    doi: 10.15283/ijsc22168

    Figure Lengend Snippet: Effects of physiological hypoxia (physioxia) on proliferation, spontaneous differentiation and cell cycle phase distribution of fetal mNSCs. (A) Representative bright-field images of E13.5 fetal mNSCs cultured for 6 or 13 days in 3% or 21% O 2 . Scale bar is 25 μm. (B) Representative images of mNSCs stained for Tuj1, Map2, GFAP, Nestin and Hoechst. A total of 10 5 cells were seeded on a coverslip and grown for three days under physioxic or normoxic conditions. Scale bar is 50 μm. (C) Comparison of a marker selection of midbrain NSC development (from left to right side) showed additional reduction of late neuronal markers such as Lmo3 or Sox6 . (D) Quantitative analysis of immunoreactivity of mNSCs grown in 3% or 21% O 2 for Tuj1, Map2, GFAP and Nestin normalised to the total number of cells (Hoechst + ) in percent. (E) Quantitative analysis of the fraction of mNSCs in the different cell cycle phases of mNSCs grown in 3 and 21% O 2 . Plots show the relative distribution of cells grown under 3% or 21% O 2 across the different cell cycle phases G0-G1, S or G2-M in percent. (F, G) Comparison of cell cycle markers (F) and senescence/quiescence markers (G) of mNSCs by microarray analysis. Heat-maps represent the fold change of the relative mRNA expression levels of mNSCs cultured under physioxia for 48 h or 13 d in comparison to those cultured under normoxia. Colour bar displays the colour contrast level of the heat-map with red and green indicating high and low expression levels for (C, F, G). *p<0.05, **p<0.01, ***p<0.001 in respect to 21% O 2 (unpaired two-sided t-test with Bonferroni correction).

    Article Snippet: Microarray chips were then immediately scanned using an Agilent microarray confocal laser scanner.

    Techniques: Cell Culture, Staining, Comparison, Marker, Selection, Microarray, Expressing

    The Notch pathway is active in mNSCs. (A) Representative images of mNSCs stained for NICD and Hoechst. A total of 10 5 cells were seeded on a coverslip and cultured for three days under hypoxic or normoxic conditions. Scale bar is 50 μm. (B) Comparison of mRNA expression of mNSCs by microarray analysis. Heat-map represents the log of the fold change of the relative mRNA expression levels of mNSCs cultured under physioxia for 48 h or 13 d in comparison to those cultured under normoxia. Colour bar displays the colour contrast level of the heat-map with red and green indicating high and low expression levels. (C) Relative mRNA levels of Hes1, 3, 5, Id1, Hey1 and Hif-1α target genes Vegf, and Pgk1 in respect to Hmbs levels as housekeeping gene of mNSCs grown in 3% or 21% O 2 . (D) Relative mRNA levels of Hes1, 3, 5, Hey1, and Notch 1 and Hif-1α target genes Vegf, and Pgk1 in respect to Hmbs, normalised to the respective control condition (Hif lox/lox ) of mNSCs Hif-1α CKO grown in 3% or 21% O 2 . Red dotted line indicates threshold for relevant changed genes. *p<0.05, **p<0.01 and ***p<0.001 in respect to normoxia (B, C) or control condition Hif lox/lox (D; unpaired two-sided t-test with Bonferroni correction).

    Journal: International Journal of Stem Cells

    Article Title: Notch Is Not Involved in Physioxia-Mediated Stem Cell Maintenance in Midbrain Neural Stem Cells

    doi: 10.15283/ijsc22168

    Figure Lengend Snippet: The Notch pathway is active in mNSCs. (A) Representative images of mNSCs stained for NICD and Hoechst. A total of 10 5 cells were seeded on a coverslip and cultured for three days under hypoxic or normoxic conditions. Scale bar is 50 μm. (B) Comparison of mRNA expression of mNSCs by microarray analysis. Heat-map represents the log of the fold change of the relative mRNA expression levels of mNSCs cultured under physioxia for 48 h or 13 d in comparison to those cultured under normoxia. Colour bar displays the colour contrast level of the heat-map with red and green indicating high and low expression levels. (C) Relative mRNA levels of Hes1, 3, 5, Id1, Hey1 and Hif-1α target genes Vegf, and Pgk1 in respect to Hmbs levels as housekeeping gene of mNSCs grown in 3% or 21% O 2 . (D) Relative mRNA levels of Hes1, 3, 5, Hey1, and Notch 1 and Hif-1α target genes Vegf, and Pgk1 in respect to Hmbs, normalised to the respective control condition (Hif lox/lox ) of mNSCs Hif-1α CKO grown in 3% or 21% O 2 . Red dotted line indicates threshold for relevant changed genes. *p<0.05, **p<0.01 and ***p<0.001 in respect to normoxia (B, C) or control condition Hif lox/lox (D; unpaired two-sided t-test with Bonferroni correction).

    Article Snippet: Microarray chips were then immediately scanned using an Agilent microarray confocal laser scanner.

    Techniques: Staining, Cell Culture, Comparison, Expressing, Microarray

    Verification of specificity . Microarrays were printed with 1816 oligonucleotides in duplicate. Each sub-grid has 13 columns and 8 rows. (A) SYTO59 DNA staining demonstrating the layout of one sub-grid. The spot in the upper right corner and the spot four columns to the left were printed with Cy3-labeled oligonucleotides for orientation purposes. Some positions near the top of each sub-grid were printed with buffer only, and are thus not visible on SYTO59 staining. (B)-(F): cDNAs contain the complementary strand of one of the 50mers on the array were amplified by PCR and labeled with Cy3. Hybridization of these cDNAs to the microarray shows that only the complementary spot had fluorescent signal in most cases. cDNA for Rn.2401 also hybridized to another spot in the same sub-grid, but with lower intensity.

    Journal: BMC Bioinformatics

    Article Title: Oliz, a suite of Perl scripts that assist in the design of microarrays using 50mer oligonucleotides from the 3' untranslated region

    doi: 10.1186/1471-2105-3-27

    Figure Lengend Snippet: Verification of specificity . Microarrays were printed with 1816 oligonucleotides in duplicate. Each sub-grid has 13 columns and 8 rows. (A) SYTO59 DNA staining demonstrating the layout of one sub-grid. The spot in the upper right corner and the spot four columns to the left were printed with Cy3-labeled oligonucleotides for orientation purposes. Some positions near the top of each sub-grid were printed with buffer only, and are thus not visible on SYTO59 staining. (B)-(F): cDNAs contain the complementary strand of one of the 50mers on the array were amplified by PCR and labeled with Cy3. Hybridization of these cDNAs to the microarray shows that only the complementary spot had fluorescent signal in most cases. cDNA for Rn.2401 also hybridized to another spot in the same sub-grid, but with lower intensity.

    Article Snippet: The arrays were spun dry and were scanned using a confocal microarray scanner (BioRad, Hercules, CA).

    Techniques: Staining, Labeling, Amplification, Hybridization, Microarray

    Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide microarray in A. Data are represented as mean ± SEM.

    Journal: iScience

    Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function

    doi: 10.1016/j.isci.2019.10.061

    Figure Lengend Snippet: Glycan Array Analysis of AgRP Binding to Heparan Sulfate Oligosaccharides (A) Fluorescent image of the glass slide glycan arrays showing fluorescence signals (green dots) of AgRP binding to 52 immobilized heparan sulfate oligosaccharides (low-molecular-weight heparan sulfate, LMHS). (B) Bar graph showing the relative fluorescence intensity of AgRP binding to the heparan sulfate oligosaccharides arrays. Heparan sulfate oligosaccharides 22 and 41 show the highest intensity. (C) The structures of the different heparan sulfate oligosaccharides on the slide microarray in A. Data are represented as mean ± SEM.

    Article Snippet: The washed arrays were dried by centrifugation and immediately scanned for green fluorescence using an InnoScan confocal microarray scanner (Innopsys, Carbonne, France).

    Techniques: Binding Assay, Fluorescence, Molecular Weight, Microarray