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Azenta codon optimization tool
Codon Optimization Tool, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/codon+optimization+tools/codon+optimized/pmc12875667-254-12-16
Average 86 stars, based on 1 article reviews
codon optimization tool - by Bioz Stars, 2026-09
86/100 stars

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Plasmid Preparation:

Article Title: Promoter and use thereof
Article Snippet: .. The codon optimization was conducted on the lacZα gene (SEQ ID NO: 39) in a pUC57 plasmid using codon optimization software (developed by Genewiz Inc. Suzhou), where the optimized lacZα gene was synthesized by Genewiz Inc. Suzhou. ..

Article Title: A fungal effector targets the chloroplast to support biotrophy by balancing disease and plant health
Article Snippet: .. The coding sequence of UmPce3, codon-optimized and synthesized for N. benthamiana (GENEWIZ), was cloned into a pCAMBIA-based vector containing the N-terminal coding sequence of luciferase (Vector 2C) using a SpeI/KpnI double-restriction digest. .. In parallel, AtRH3 was amplified using primers containing DraIII restriction sites, as detailed in Supplemental Table 5 and cloned into the pCAMBIA vector carrying the C-terminal coding sequence of luciferase (Vector 2B) and ZmRh3b (codon-optimized and synthesized for N. benthamiana by GENEWIZ) was cloned into the Vector 2B by GENEWIZ.

Article Title: Coiled-coil centrosomal proteins facilitate tubulin concentration via polyKR motifs.
Article Snippet: 1 Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan 2 Department of Infectious Diseases, Virology, Heidelberg University, Germany 3 Max Planck School Matter to Life, Heidelberg, Germany 4 Protein Expression and Purification Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany 5 Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, CA, USA

Article Title: Conserved stem-loops of the SARS-CoV-2 5’-UTR activate OAS1
Article Snippet: .. Plasmid DNA encoding nucleotides 1-3621 from strain SARS-CoV-2/human/USA/GA-PGCoE-0083E/2024 (PQ331003.1) (Genewiz) was used as template for PCR amplification of the full 5’-SE (SL1-8) region and 3’-end truncation variants (SL-7 to SL1-3) using primers listed in ( Supplemental Table S1 ). ..

Software:

Article Title: Promoter and use thereof
Article Snippet: .. The codon optimization was conducted on the lacZα gene (SEQ ID NO: 39) in a pUC57 plasmid using codon optimization software (developed by Genewiz Inc. Suzhou), where the optimized lacZα gene was synthesized by Genewiz Inc. Suzhou. ..

Synthesized:

Article Title: Promoter and use thereof
Article Snippet: .. The codon optimization was conducted on the lacZα gene (SEQ ID NO: 39) in a pUC57 plasmid using codon optimization software (developed by Genewiz Inc. Suzhou), where the optimized lacZα gene was synthesized by Genewiz Inc. Suzhou. ..

Article Title: A fungal effector targets the chloroplast to support biotrophy by balancing disease and plant health
Article Snippet: .. The coding sequence of UmPce3, codon-optimized and synthesized for N. benthamiana (GENEWIZ), was cloned into a pCAMBIA-based vector containing the N-terminal coding sequence of luciferase (Vector 2C) using a SpeI/KpnI double-restriction digest. .. In parallel, AtRH3 was amplified using primers containing DraIII restriction sites, as detailed in Supplemental Table 5 and cloned into the pCAMBIA vector carrying the C-terminal coding sequence of luciferase (Vector 2B) and ZmRh3b (codon-optimized and synthesized for N. benthamiana by GENEWIZ) was cloned into the Vector 2B by GENEWIZ.

Article Title: Coiled-coil centrosomal proteins facilitate tubulin concentration via polyKR motifs.
Article Snippet: 1 Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan 2 Department of Infectious Diseases, Virology, Heidelberg University, Germany 3 Max Planck School Matter to Life, Heidelberg, Germany 4 Protein Expression and Purification Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany 5 Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, CA, USA

Article Title: A fungal effector targets the chloroplast to support biotrophy by balancing disease and plant health
Article Snippet: .. For constitutive heterologous expression of UmPce3 (UMAG_05298) in Arabidopsis, a GENEWIZ-codon-optimized UmPce3 sequence was synthesized and cloned by GENEWIZ into the pBASTA-35S-3HA vector. ..

Article Title: WTR: A Toolkit for Functional Anterograde Transsynaptic Circuit Mapping
Article Snippet: EF1α-DIO-hGluc-CMV-CLuc was made from pC0037 (Addgene, #181934). .. The codon-optimized WGA (mWGA) sequence was synthesized by Genewiz Inc. (South Plainfield, NJ) and fused with a TEVp cleavage site (GAGAACCTGTACTTCCAGGGC), along with either Cre or Flpo recombinase. ..

Clone Assay:

Article Title: Genetically engineered Streptomyces viridosporus ATCC 14672 strains for the discovery of novel moenomycins.
Article Snippet: .. The codon-optimized synthetic gene plu3366, cloned into vector pUC57-kan, was ordered from Genewiz (South Plainfield, NJ); the nucleotide sequences of the natural and synthetic genes are given in Fig. S1, ESM. ..

Article Title: A fungal effector targets the chloroplast to support biotrophy by balancing disease and plant health
Article Snippet: .. The coding sequence of UmPce3, codon-optimized and synthesized for N. benthamiana (GENEWIZ), was cloned into a pCAMBIA-based vector containing the N-terminal coding sequence of luciferase (Vector 2C) using a SpeI/KpnI double-restriction digest. .. In parallel, AtRH3 was amplified using primers containing DraIII restriction sites, as detailed in Supplemental Table 5 and cloned into the pCAMBIA vector carrying the C-terminal coding sequence of luciferase (Vector 2B) and ZmRh3b (codon-optimized and synthesized for N. benthamiana by GENEWIZ) was cloned into the Vector 2B by GENEWIZ.

Article Title: Coiled-coil centrosomal proteins facilitate tubulin concentration via polyKR motifs.
Article Snippet: 1 Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan 2 Department of Infectious Diseases, Virology, Heidelberg University, Germany 3 Max Planck School Matter to Life, Heidelberg, Germany 4 Protein Expression and Purification Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany 5 Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, CA, USA

Article Title: A fungal effector targets the chloroplast to support biotrophy by balancing disease and plant health
Article Snippet: .. For constitutive heterologous expression of UmPce3 (UMAG_05298) in Arabidopsis, a GENEWIZ-codon-optimized UmPce3 sequence was synthesized and cloned by GENEWIZ into the pBASTA-35S-3HA vector. ..

Sequencing:

Article Title: A fungal effector targets the chloroplast to support biotrophy by balancing disease and plant health
Article Snippet: .. The coding sequence of UmPce3, codon-optimized and synthesized for N. benthamiana (GENEWIZ), was cloned into a pCAMBIA-based vector containing the N-terminal coding sequence of luciferase (Vector 2C) using a SpeI/KpnI double-restriction digest. .. In parallel, AtRH3 was amplified using primers containing DraIII restriction sites, as detailed in Supplemental Table 5 and cloned into the pCAMBIA vector carrying the C-terminal coding sequence of luciferase (Vector 2B) and ZmRh3b (codon-optimized and synthesized for N. benthamiana by GENEWIZ) was cloned into the Vector 2B by GENEWIZ.

Article Title: A fungal effector targets the chloroplast to support biotrophy by balancing disease and plant health
Article Snippet: .. For constitutive heterologous expression of UmPce3 (UMAG_05298) in Arabidopsis, a GENEWIZ-codon-optimized UmPce3 sequence was synthesized and cloned by GENEWIZ into the pBASTA-35S-3HA vector. ..

Article Title: WTR: A Toolkit for Functional Anterograde Transsynaptic Circuit Mapping
Article Snippet: EF1α-DIO-hGluc-CMV-CLuc was made from pC0037 (Addgene, #181934). .. The codon-optimized WGA (mWGA) sequence was synthesized by Genewiz Inc. (South Plainfield, NJ) and fused with a TEVp cleavage site (GAGAACCTGTACTTCCAGGGC), along with either Cre or Flpo recombinase. ..

Luciferase:

Article Title: A fungal effector targets the chloroplast to support biotrophy by balancing disease and plant health
Article Snippet: .. The coding sequence of UmPce3, codon-optimized and synthesized for N. benthamiana (GENEWIZ), was cloned into a pCAMBIA-based vector containing the N-terminal coding sequence of luciferase (Vector 2C) using a SpeI/KpnI double-restriction digest. .. In parallel, AtRH3 was amplified using primers containing DraIII restriction sites, as detailed in Supplemental Table 5 and cloned into the pCAMBIA vector carrying the C-terminal coding sequence of luciferase (Vector 2B) and ZmRh3b (codon-optimized and synthesized for N. benthamiana by GENEWIZ) was cloned into the Vector 2B by GENEWIZ.

Polymerase Chain Reaction:

Article Title: Conserved stem-loops of the SARS-CoV-2 5’-UTR activate OAS1
Article Snippet: .. Plasmid DNA encoding nucleotides 1-3621 from strain SARS-CoV-2/human/USA/GA-PGCoE-0083E/2024 (PQ331003.1) (Genewiz) was used as template for PCR amplification of the full 5’-SE (SL1-8) region and 3’-end truncation variants (SL-7 to SL1-3) using primers listed in ( Supplemental Table S1 ). ..

Amplification:

Article Title: Conserved stem-loops of the SARS-CoV-2 5’-UTR activate OAS1
Article Snippet: .. Plasmid DNA encoding nucleotides 1-3621 from strain SARS-CoV-2/human/USA/GA-PGCoE-0083E/2024 (PQ331003.1) (Genewiz) was used as template for PCR amplification of the full 5’-SE (SL1-8) region and 3’-end truncation variants (SL-7 to SL1-3) using primers listed in ( Supplemental Table S1 ). ..

Expressing:

Article Title: A fungal effector targets the chloroplast to support biotrophy by balancing disease and plant health
Article Snippet: .. For constitutive heterologous expression of UmPce3 (UMAG_05298) in Arabidopsis, a GENEWIZ-codon-optimized UmPce3 sequence was synthesized and cloned by GENEWIZ into the pBASTA-35S-3HA vector. ..

Whole Genome Amplification:

Article Title: WTR: A Toolkit for Functional Anterograde Transsynaptic Circuit Mapping
Article Snippet: EF1α-DIO-hGluc-CMV-CLuc was made from pC0037 (Addgene, #181934). .. The codon-optimized WGA (mWGA) sequence was synthesized by Genewiz Inc. (South Plainfield, NJ) and fused with a TEVp cleavage site (GAGAACCTGTACTTCCAGGGC), along with either Cre or Flpo recombinase. ..



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