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Blue Heron Biotech codon/expression optimization tool
Codon/Expression Optimization Tool, supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/codon+optimization+tool/blue+heron+biotech+online+codon+optimization+tool/pmc05799272-99-19-22
Average 90 stars, based on 1 article reviews
codon/expression optimization tool - by Bioz Stars, 2026-09
90/100 stars

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other:

Article Title: Structural analysis of free and liganded forms of the Fab fragment of a high-affinity anti-cocaine antibody, h2E2
Article Snippet: Amino-acid sequences for the h2E2 heavy-chain and light-chain variable domains as well as for the human lambda 2 constant domain were reverse-transcribed and optimized for expression in E. coli using the Blue Heron Biotech online codon-optimization tool ( https://www.blueheronbio.com/codon-optimization ).

Article Title: Plasmodium PK9 Inhibitors Promote Growth of Liver Stage Parasites
Article Snippet: Recombinant Protein Expression and Purification Codon optimized Pf PK9-His (BlueHeron) in pET21a, Pf UBC13-His 6 (amplified from P. falciparum cDNA) in pET21a, and GST- Hs UBC13 in pGEX6 (kind gift from Dr. Wei Xiao at the University of Saskatchewan)( McKenna et al., 2001 ) were expressed in E. coli BL21(DE3).

Article Title: An integrated approach reveals a regulatory mechanism coupling Src’s kinase activity, localization, and phosphotransferase-independent functions
Article Snippet: Yeast growth assay To confirm that Src expression affects growth when expressed in S. cerevisiae , codon-optimized full-length human Src myr (Blue Heron Biotech) and indicated mutants were transformed into the S. cerevisiae BY4741 strain using standard LiAc transformation protocols ( Gietz and Schiestl, 2007 ) and plated on C-Leu plates to select for successful transformants.

Article Title: Plasmodium PK9 Inhibitors Promote Growth of Liver Stage Parasites
Article Snippet: Codon optimized Pf PK9-His (BlueHeron) in pET21a, Pf UBC13-His 6 (amplified from P. falciparum cDNA) in pET21a, and GST- Hs UBC13 in pGEX6 (kind gift from Dr. Wei Xiao at the University of Saskatchewan)( McKenna et al., 2001 ) were expressed in E. coli BL21(DE3).

Article Title: An integrated approach reveals a regulatory mechanism coupling Src’s kinase activity, localization, and phosphotransferase-independent functions
Article Snippet: To confirm that Src expression affects growth when expressed in S. cerevisiae , codon-optimized full-length human Src myr (Blue Heron Biotech) and indicated mutants were transformed into the S. cerevisiae BY4741 strain using standard LiAc transformation protocols ( Gietz and Schiestl, 2007 ) and plated on C-Leu plates to select for successful transformants.

Article Title: Structural analysis of free and liganded forms of the Fab fragment of a high-affinity anti-cocaine antibody, h2E2
Article Snippet: Vector construction Amino-acid sequences for the h2E2 heavy-chain and light-chain variable domains as well as for the human lambda 2 constant domain were reverse-transcribed and optimized for expression in E. coli using the Blue Heron Biotech online codon-optimization tool ( https://www.blueheronbio.com/codon-optimization ).

Expressing:

Article Title: Silica Nanodepletors: Targeting and Clearing Alzheimer's β‐Amyloid Plaques
Article Snippet: .. Construction of the 1E8 scFv Expression Vector: The 1E8 scFv amino acid sequence was reverse translated using the Codon Optimization Tool (Blue Heron Biotech, LLC, Bothell, WA) in order to maximize expression in E. coli. ..

Article Title: Characterization of 3-[(Carboxymethyl)thio]picolinic Acid: A Novel Inhibitor of Phosphoenolpyruvate Carboxykinase.
Article Snippet: Phosphoenolpyruvate carboxykinase (PEPCK) activity has traditionally been characterized for its role as the first committed step of gluconeogenesis.. The current understanding of PEPCK’s metabolic role has recently expanded as a general mediator of tricarboxylic acid cycle flux.. Selective inhibition of PEPCK in vivo and in vitro has been with 3-mercaptopicolinic acid (MPA) (Ki~8μM), whose mechanism of inhibition has only been recently elucidated.

Sequencing:

Article Title: Silica Nanodepletors: Targeting and Clearing Alzheimer's β‐Amyloid Plaques
Article Snippet: .. Construction of the 1E8 scFv Expression Vector: The 1E8 scFv amino acid sequence was reverse translated using the Codon Optimization Tool (Blue Heron Biotech, LLC, Bothell, WA) in order to maximize expression in E. coli. ..

Article Title: Characterization of 3-[(Carboxymethyl)thio]picolinic Acid: A Novel Inhibitor of Phosphoenolpyruvate Carboxykinase.
Article Snippet: Phosphoenolpyruvate carboxykinase (PEPCK) activity has traditionally been characterized for its role as the first committed step of gluconeogenesis.. The current understanding of PEPCK’s metabolic role has recently expanded as a general mediator of tricarboxylic acid cycle flux.. Selective inhibition of PEPCK in vivo and in vitro has been with 3-mercaptopicolinic acid (MPA) (Ki~8μM), whose mechanism of inhibition has only been recently elucidated.

Clone Assay:

Article Title: Characterization of 3-[(Carboxymethyl)thio]picolinic Acid: A Novel Inhibitor of Phosphoenolpyruvate Carboxykinase.
Article Snippet: Phosphoenolpyruvate carboxykinase (PEPCK) activity has traditionally been characterized for its role as the first committed step of gluconeogenesis.. The current understanding of PEPCK’s metabolic role has recently expanded as a general mediator of tricarboxylic acid cycle flux.. Selective inhibition of PEPCK in vivo and in vitro has been with 3-mercaptopicolinic acid (MPA) (Ki~8μM), whose mechanism of inhibition has only been recently elucidated.



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