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GenScript corporation gensmart codon optimization algorithm
Gensmart Codon Optimization Algorithm, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/codon+optimization+algorithm/gensmart+codon+optimization/pmc11289437-54-15-21
Average 90 stars, based on 1 article reviews
gensmart codon optimization algorithm - by Bioz Stars, 2026-09
90/100 stars

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Sequencing:

Article Title: The genome of Chinese medicinal herb Fuling reveals a key enzyme CYP5035D1C31 involved in pachymic acid biosynthesis.
Article Snippet: Fuling (Wolfiporia hoelen) is a widely used traditional Chinese herbal medicine.. Pachymic acid is the representative bioactive lanosterol triterpenoid in Fuling, with the potential for developing anti-tumor drugs.. However, the gene involved in the modification of lanosterol is unclear.

Article Title: Trans amplifying mRNA vaccine expressing consensus spike elicits broad neutralization of SARS CoV 2 variants.
Article Snippet: Plasmids for consensus spike and replicase The VEEV replicase sequence was sourced from plasmid pTK194, kindly provided by RonWeiss (Addgene plasmid #59563; http://n2t.net/addgene: 59563; RRID)74. .. This sequence was codon-optimized for expression in human cells using the GenSmartTM Codon Optimization Tool (Genscript, Fig. 5 | Transcriptomic analysis of A549 cells transfected with VEEV replicase mRNA and SARS-CoV-2 mRNA (treatment group, n= 3) compared to cells transfected with an unrelated mRNA and SARS-CoV-2 mRNA (control group, n= 3). ..

Expressing:

Article Title: Trans amplifying mRNA vaccine expressing consensus spike elicits broad neutralization of SARS CoV 2 variants.
Article Snippet: Plasmids for consensus spike and replicase The VEEV replicase sequence was sourced from plasmid pTK194, kindly provided by RonWeiss (Addgene plasmid #59563; http://n2t.net/addgene: 59563; RRID)74. .. This sequence was codon-optimized for expression in human cells using the GenSmartTM Codon Optimization Tool (Genscript, Fig. 5 | Transcriptomic analysis of A549 cells transfected with VEEV replicase mRNA and SARS-CoV-2 mRNA (treatment group, n= 3) compared to cells transfected with an unrelated mRNA and SARS-CoV-2 mRNA (control group, n= 3). ..

Article Title: Chimeric designs of rabies virus glycoprotein G enhance baculovirus pseudotyping and immunogenicity in mice: influence of the transmembrane domain and a flexible linker.
Article Snippet: .. All sequences were codon-optimized for expression in Spodoptera frugiperda using the GenSmartTM Codon Optimization Tool (GenScript, USA). .. The optimized sequences were chemically synthesized by GenScript and cloned into the pFastBAc1 plasmid using BamHI and HindIII restriction sites.

Article Title: Computational design of a multi-epitope mRNA vaccine against orthopoxviruses: A path toward comprehensive poxvirus protection.
Article Snippet: Along with the recent outbreak of monkeypox (MPOX), there are worries about other outbreaks of the poxvirus genus that threaten global public health.. Until now, although there are no specific vaccines for MPOXV, there are no comprehensive vaccines for all dangerous poxviruses.. This research utilizes immunoinformatics and structural vaccinology methodologies to develop a multi-epitope mRNA vaccine targeting variola virus (VARV), vaccinia virus (VACV), monkeypox virus (MPOXV), and cowpox virus (CPXV), which are four pathogenic orthopoxviruses (OPV).

Transfection:

Article Title: Trans amplifying mRNA vaccine expressing consensus spike elicits broad neutralization of SARS CoV 2 variants.
Article Snippet: Plasmids for consensus spike and replicase The VEEV replicase sequence was sourced from plasmid pTK194, kindly provided by RonWeiss (Addgene plasmid #59563; http://n2t.net/addgene: 59563; RRID)74. .. This sequence was codon-optimized for expression in human cells using the GenSmartTM Codon Optimization Tool (Genscript, Fig. 5 | Transcriptomic analysis of A549 cells transfected with VEEV replicase mRNA and SARS-CoV-2 mRNA (treatment group, n= 3) compared to cells transfected with an unrelated mRNA and SARS-CoV-2 mRNA (control group, n= 3). ..

Control:

Article Title: Trans amplifying mRNA vaccine expressing consensus spike elicits broad neutralization of SARS CoV 2 variants.
Article Snippet: Plasmids for consensus spike and replicase The VEEV replicase sequence was sourced from plasmid pTK194, kindly provided by RonWeiss (Addgene plasmid #59563; http://n2t.net/addgene: 59563; RRID)74. .. This sequence was codon-optimized for expression in human cells using the GenSmartTM Codon Optimization Tool (Genscript, Fig. 5 | Transcriptomic analysis of A549 cells transfected with VEEV replicase mRNA and SARS-CoV-2 mRNA (treatment group, n= 3) compared to cells transfected with an unrelated mRNA and SARS-CoV-2 mRNA (control group, n= 3). ..

Construct:

Article Title: Computational design of a multi-epitope mRNA vaccine against orthopoxviruses: A path toward comprehensive poxvirus protection.
Article Snippet: Along with the recent outbreak of monkeypox (MPOX), there are worries about other outbreaks of the poxvirus genus that threaten global public health.. Until now, although there are no specific vaccines for MPOXV, there are no comprehensive vaccines for all dangerous poxviruses.. This research utilizes immunoinformatics and structural vaccinology methodologies to develop a multi-epitope mRNA vaccine targeting variola virus (VARV), vaccinia virus (VACV), monkeypox virus (MPOXV), and cowpox virus (CPXV), which are four pathogenic orthopoxviruses (OPV).

other:

Article Title: Supporting Information for Synthesis of Bioengineered Heparin Chemically and Biologically Similar to Porcine-Derived Products and Convertible to Low MW Heparin
Article Snippet: Next, the protein sequences were reverse-translated, and the resulting DNA sequence was codon-optimized for expression in E. coli (GenSmart Codon Optimization, GenScript).



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(A) codon harmonization (CH) (B) Genewiz. (C) JCat.

Journal: bioRxiv

Article Title: Impact of different synonymous codon substitution strategies on SARS-CoV-2 nucleocapsid protein expression in Escherichia coli

doi: 10.1101/2024.11.06.622014

Figure Lengend Snippet: (A) codon harmonization (CH) (B) Genewiz. (C) JCat.

Article Snippet: In this study, we performed synonymous codon substitution and compared the underlining parameters affecting SARS-CoV-2 nucleocapsid protein expression using JCat and Genewiz codon optimization algorithms, and codon harmonization tool.

Techniques: