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Nature Biotechnology encode chip-seq data sets
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Titration:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

Chromatin Immunoprecipitation:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

shRNA:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

RNA Sequencing:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

Expressing:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

Control:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

Gene Expression:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

Genome Wide:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

Purification:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

FACS:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

In Vitro:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.

Modification:

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology Article https://doi.org/10.1038/s41587-022-01560-3 Extended Data Fig. 2 | scChIX-seq accurately deconvolves double-incubated signal into their respective histone modifications. (a) Histogram of unique fragment cuts per cell. (b) Histogram of fraction of unique fragments starting with a “TA” motif. (c, d) UMAP of latent Dirichlet allocation (LDA) embedding using k=30 topics for H3K27me3 (c) and H3K9me3 (d). (e, f ) UMAP representation of H3K27me3 (left) and H3K9me3 (right) data colored by unmixed or single-incubated cells (e) or ground truth cell type labels defined by FACS (f). (g, h) Genome-wide Pearson correlation between deconvolved H3K27me3 (g) and H3K9me3 (h) signal versus ground truth sortChIC purified by FACS.

Article Title: Chromatin and Nucleosome-Associated Features in Liquid Biopsy: Implications for Cancer Biomarker Discovery.
Article Snippet: 14 Cell-free DNA (cfDNA) from the bloodstream has been studied for cancer biomarker 15 discovery, and chromatin-derived epigenetic features have come into the spotlight for 16 their potential to expand clinical applications.. Methylation, fragmentation, and 17 nucleosome positioning patterns of cfDNA have previously been shown to reveal 18 epigenomic and inferred transcriptomic information.. More recently, histone modifications 19 have emerged as a tool to further identify tumor-specific chromatin variants in plasma.

Article Title: The Wild West of spike-in normalization.
Article Snippet: Spike-in normalization is a powerful approach to assess global changes in data obtained from the genomic mapping of DNA-associated proteins by methods such as chromatin immunoprecipitation with sequencing (ChIP-seq) or CUT&RUN.. It was developed to correctly quantify protein–DNA interactions in cases where the overall concentration of target-DNA-associated proteins differs significantly between samples.. Spiking in exogenous chromatin from another species before immunoprecipitation (IP) provides an internal control, with the assumption that the epitope of interest does not vary in the added exogenous material.

Article Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution.
Article Snippet: We next sought to apply NTT-seq in a complex tissue that contains differentiating cells to capture chromatin remodeling dynamics that Nature Biotechnology | Volume 41 | June 2023 | 806–812 809 Article https://doi.org/10.1038/s41587-022-01588-5 individually in bulk-cell NTT-seq experiments using K562 cells. d, Scatter plots showing normalized fragment counts for H3K27me3, H3K27ac and RNAPII peaks defined by ENCODE17, for bulk-cell and single-cell multiplexed NTT-seq experiments, for K562 cells.

Article Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens.
Article Snippet: Nature Biotechnology Brief Communication https://doi.org/10.1038/s41587-023-01948-9 Public ChIP-seq, shRNA RNA-seq and GRO-seq data analysis Genes with detectable expression were identified from shControl/ shAGO2 bulk RNA-seq in ENCODE.

Article Title: scChIX-seq infers dynamic relationships between histone modifications in single cells.
Article Snippet: Nature Biotechnology | Volume 41 | June 2023 | 813–823 813 nature biotechnology https://doi.org/10.1038/s41587-022-01560-3Article scChIX-seq infers dynamic relationships between histone modifications in single cells Jake Yeung 1,2,3 , Maria Florescu 1,3, Peter Zeller1,3, Buys Anton de Barbanson 1, Max D. Wellenstein1 & Alexander van Oudenaarden 1 Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression.

Article Title: CD137 Protein Expression Pattern Determines the Functional Role of Galectin-9 in Colorectal Cancer.
Article Snippet: D. Sun, X. Guan, A. E. Moran, et al., “Identifying Phenotype‐ Associated Subpopulations by Integrating Bulk and Single‐Cell Sequencing Data,” Nature Biotechnology 40, no. 4 (2022): 527–538.

Article Title: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.
Article Snippet: This limitation was addressed in 2015, when a landmark paper by David Weitz and Bradley E Bernstein in Nature Biotechnology introduced the concept of single-cell ChIP-Seq108.



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