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4 6 diamidino 2 phenylindole  (Dojindo Labs)


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    Structured Review

    Dojindo Labs 4 6 diamidino 2 phenylindole
    4 6 Diamidino 2 Phenylindole, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellstain+dapi+solution/Cellstain+DAPI+solution/pm42863997-63-16-24
    Average 96 stars, based on 495 article reviews
    4 6 diamidino 2 phenylindole - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: A CD4 + T cell-fibroblast crosstalk exacerbates autoimmunity in a mouse model of primary Sjögren disease.
    Article Snippet: To evaluate nuclear translocation of NF-κB p65, fibroblasts (1×105 cells/well) from WT- or Tnfrsf8-deficient pSjD model mouse SG were first AR TI CL E IN P RE SS cultured in DMEM containing 10% FBS on collagen coated-culture cover glass (Matsunami Glass Ind., Ltd., Cat# CS1163) placed in a 24-well plate for one day.

    Article Title: 4-Phenylbutyric acid activates an NF-κB-Egr-1 axis to control myoblast proliferation and ECM gene expression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Egr-1 Proteintech Cat #: 55117-1-AP; RRID: AB_2881272 Mouse anti-Fos Proteintech Cat #: 66590-1-IG; RRID: AB_2881950 Rabbit anti-MYH1, clone 1E15 Sigma-Aldrich Cat #: ZRB1214; RRID: AB_3083662 Rabbit anti-MYH7, clone 1B19 Sigma-Aldrich Cat #: ZRB1308 Mouse anti-NCL-Hamlet DYSF Leica Biosystems Cat #: NCL-Hamlet; RRID: AB_442097 Mouse anti-Myogenin BD Biosciences Cat #: 556358; RRID: AB_396383 Rabbit anti-Desmin Proteintech Cat #: 16520-1-AP; RRID: AB_2292918 Rabbit anti-β-Actin GeneTex Cat #: GTX110564; RRID: AB_10618080 Rabbit anti-Phospho- ERK1/2 (Thr202/Tyr204) Proteintech Cat #: 28733-1-AP; RRID: AB_2881202 Rabbit anti-Erk1/2 Proteintech Cat #: 11257-1-AP; RRID: AB_2139822 Rabbit anti-Phospho-NF-κB p65 (Ser468) Proteintech Cat #: 82335-1-RR; RRID: AB_3083091 Rabbit anti-Phospho-NF-kB p65 (Ser536) Cell Signaling Technology Cat #: 3033; RRID: AB_331284 Rabbit anti-Acetyl-NFkB p65 (Lys310) Invitrogen Cat #: PA5-17264; RRID: AB_10983502 Rabbit anti-NF-κB p65 BioLegend Cat #: 622602; RRID: AB_315956 Rabbit anti-Histone H3 (D1H2) Cell Signaling Technology Cat #: 4499; RRID: AB_10544537 Rabbit anti-Acetyl-Histone H3 (Lys9) (C5B11) Cell Signaling Technology Cat #: 9649; RRID: AB_823528 Rabbit anti-Acetyl-Histone H3 (Lys14) (D4B9) Cell Signaling Technology Cat #: 7627; RRID: AB_10839410 Rabbit anti-Acetyl-Histone H3 (Lys18) (D8Z5H) Cell Signaling Technology Cat #: 13998; RRID: AB_2783723 Rabbit anti-Acetyl-Histone H3 (Lys27) (D5E4) Cell Signaling Technology Cat #: 8173; RRID: AB_10949503 Rabbit anti-Acetyl-Histone H3 (Lys56) Cell Signaling Technology Cat #: 4243; RRID: AB_10548193 Rabbit anti-HDAC5 (D1J7V) Cell Signaling Technology Cat #: 20458; RRID: AB_2713973 Rabbit anti-Phospho-eIF2α (Ser51) (D9G8) Cell Signaling Technology Cat #: 3398T; RRID: AB_2096481 Rabbit anti-eIF2α (D7D3) Cell Signaling Technology Cat #: 5324T; RRID: AB_10692650 Rabbit anti-Grp78 GeneTex Cat #: GTX113340; RRID: AB_11173017 Rabbit anti-ATF4 GeneTex Cat #: GTX101943; RRID: AB_1240487 Rabbit anti-GADD153 (CHOP) GeneTex Cat #: GTX112827 Rabbit anti-IRE1 (pSer724) GeneTex Cat #: GTX132808; RRID: AB_2886751 Rabbit anti-IREα (14C10) Cell Signaling Technology Cat #: 3294T; RRID: AB_823545 Mouse anti-ATF6 Antibody (70B1413.1) Novus Biologicals Cat #: NBP1-40256; RRID: AB_2058774 Rabbit anti-Pax7 [EPR30466-531] abcam Cat #: ab324017 Rabbit anti-OPA1 (D6U6N) Cell Signaling Technology Cat #: 80471T; RRID: AB_2734117 Rabbit anti-DRP1 (C-terminal) Proteintech Cat #: 12957-1-AP; RRID: AB_2093525 Mouse anti-PGC1α Proteintech Cat #: 66369-1-IG; RRID: AB_2828002 Goat anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat #: 7074S; RRID: AB_2099233 Horse anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat #: 7076S; RRID: AB_330924 Goat anti-Rabbit IgG H&L (Alexa Fluor 488) abcam Cat #: ab150077; RRID: AB_2630356 Goat anti-Mouse IgG H&L (Alexa Fluor 488) abcam Cat #: ab150113; RRID: AB_2576208 Purified anti-Rabbit IgG BioLegend Cat #: 410404; RRID: AB_2632606 Chemicals, peptides, and recombinant proteins 4-Phenylbutyric acid (4-PBA) Sigma-Aldrich Cat #: P21005-25G Block Ace blocking reagent DS Pharma Biomedical Cat #: UK-B80 Bovine serum albumin (BSA) Nacalai tesque Cat #: 01281-26 Collagenase type 1 Worthington Biochemical Corporation Cat #: CLS1 (Continued on next page) 16 iScience 29, 117620, October 16, 2026

    Sampling:

    Article Title: Impact of Host α2-Antiplasmin Deficiency on Engraftment and the Morphological Maturation of Transplanted Neural Precursor Cells in the Mouse Brain.
    Article Snippet: .. To evaluate graft size, one series (sampling interval: 180 μm) from each animal was counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1 : 800 in PBS; D523; Dojindo), mounted on glass slides, and imaged using an all-in-one fluorescence microscope (BZ-8000; Keyence, Osaka, Japan). .. The GFP-positive area was quantified as graft area using ImageJ software program (National Institutes of Health, Bethesda, MD, U.S.A.), and graft volume was estimated by multiplying the sum of GFP-positive areas by the section interval (180 μm).

    Glass:

    Article Title: Impact of Host α2-Antiplasmin Deficiency on Engraftment and the Morphological Maturation of Transplanted Neural Precursor Cells in the Mouse Brain.
    Article Snippet: .. To evaluate graft size, one series (sampling interval: 180 μm) from each animal was counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1 : 800 in PBS; D523; Dojindo), mounted on glass slides, and imaged using an all-in-one fluorescence microscope (BZ-8000; Keyence, Osaka, Japan). .. The GFP-positive area was quantified as graft area using ImageJ software program (National Institutes of Health, Bethesda, MD, U.S.A.), and graft volume was estimated by multiplying the sum of GFP-positive areas by the section interval (180 μm).

    Article Title: Dysferlin promotes inflammatory macrophage responses and modulates Ca 2+ -dependent STAT1 signaling.
    Article Snippet: .. After washing with PBS, the cells and sections were incubated with an Alexa Fluor 488 Goat Anti-Rabbit IgG H&L (ab150077; Abcam), Alexa Fluor 488 Goat Anti-Mouse IgG H&L (ab150113; Abcam), Alexa Fluor 594 Goat Anti-Rabbit IgG H&L (ab150080; Abcam), or an Alexa 594 Anti-Mouse IgG antibody (ab150116; Abcam) as a secondary antibody at room temperature for 1 h. the cells and sections were enclosed using ProLong Glass Antifade Mountant (P36965; Thermo Fisher Scientific) after staining the nuclei with 4′,6-diamidino-2phenylindole (DAPI; D523; Dojindo) at room temperature for 5 min. .. The fluorescent images were obtained under a fluorescence microscope (Keyence).

    Fluorescence:

    Article Title: Impact of Host α2-Antiplasmin Deficiency on Engraftment and the Morphological Maturation of Transplanted Neural Precursor Cells in the Mouse Brain.
    Article Snippet: .. To evaluate graft size, one series (sampling interval: 180 μm) from each animal was counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1 : 800 in PBS; D523; Dojindo), mounted on glass slides, and imaged using an all-in-one fluorescence microscope (BZ-8000; Keyence, Osaka, Japan). .. The GFP-positive area was quantified as graft area using ImageJ software program (National Institutes of Health, Bethesda, MD, U.S.A.), and graft volume was estimated by multiplying the sum of GFP-positive areas by the section interval (180 μm).

    Article Title: Novel compounds induce AChR clustering and improve motor function in myasthenia gravis animal models.
    Article Snippet: The neuromuscular junction (NMJ), the synapse between motor nerves and skeletal muscles, is implicated in the pathogenesis of various neuromuscular disorders, including myasthenia gravis (MG), congenital myasthenia, and amyotrophic lateral sclerosis.. Postsynaptic clustering of acetylcholine receptors (AChRs) is crucial for NMJ formation.. This process is initiated by agrin, a glycoprotein secreted from motor nerve terminals, which activates muscle-specific tyrosine kinase (MuSK) via its coreceptor, low-density lipoprotein receptor-related protein 4 (Lrp4), and thereby activates downstream signaling pathways that induce AChR clustering.

    Microscopy:

    Article Title: Impact of Host α2-Antiplasmin Deficiency on Engraftment and the Morphological Maturation of Transplanted Neural Precursor Cells in the Mouse Brain.
    Article Snippet: .. To evaluate graft size, one series (sampling interval: 180 μm) from each animal was counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1 : 800 in PBS; D523; Dojindo), mounted on glass slides, and imaged using an all-in-one fluorescence microscope (BZ-8000; Keyence, Osaka, Japan). .. The GFP-positive area was quantified as graft area using ImageJ software program (National Institutes of Health, Bethesda, MD, U.S.A.), and graft volume was estimated by multiplying the sum of GFP-positive areas by the section interval (180 μm).

    Article Title: Novel compounds induce AChR clustering and improve motor function in myasthenia gravis animal models.
    Article Snippet: The neuromuscular junction (NMJ), the synapse between motor nerves and skeletal muscles, is implicated in the pathogenesis of various neuromuscular disorders, including myasthenia gravis (MG), congenital myasthenia, and amyotrophic lateral sclerosis.. Postsynaptic clustering of acetylcholine receptors (AChRs) is crucial for NMJ formation.. This process is initiated by agrin, a glycoprotein secreted from motor nerve terminals, which activates muscle-specific tyrosine kinase (MuSK) via its coreceptor, low-density lipoprotein receptor-related protein 4 (Lrp4), and thereby activates downstream signaling pathways that induce AChR clustering.

    Staining:

    Article Title: Novel compounds induce AChR clustering and improve motor function in myasthenia gravis animal models.
    Article Snippet: The neuromuscular junction (NMJ), the synapse between motor nerves and skeletal muscles, is implicated in the pathogenesis of various neuromuscular disorders, including myasthenia gravis (MG), congenital myasthenia, and amyotrophic lateral sclerosis.. Postsynaptic clustering of acetylcholine receptors (AChRs) is crucial for NMJ formation.. This process is initiated by agrin, a glycoprotein secreted from motor nerve terminals, which activates muscle-specific tyrosine kinase (MuSK) via its coreceptor, low-density lipoprotein receptor-related protein 4 (Lrp4), and thereby activates downstream signaling pathways that induce AChR clustering.

    Article Title: Correlation-Based Morphometric Analysis Reveals Structural Remodeling of Platelet-Bound Circulating Tumor Cells in Urothelial Carcinoma.
    Article Snippet: Circulating tumor cells (CTCs) mediate hematogenous metastasis and are key targets of liquid biopsy.. Although platelet−tumor cell interactions enhance survival and dissemination, systematic studies of CTCs remain limited because of their rarity and heterogeneity.. We report a single-cell approach for evaluating platelet-bound CTCs.

    Article Title: Dysferlin promotes inflammatory macrophage responses and modulates Ca 2+ -dependent STAT1 signaling.
    Article Snippet: .. After washing with PBS, the cells and sections were incubated with an Alexa Fluor 488 Goat Anti-Rabbit IgG H&L (ab150077; Abcam), Alexa Fluor 488 Goat Anti-Mouse IgG H&L (ab150113; Abcam), Alexa Fluor 594 Goat Anti-Rabbit IgG H&L (ab150080; Abcam), or an Alexa 594 Anti-Mouse IgG antibody (ab150116; Abcam) as a secondary antibody at room temperature for 1 h. the cells and sections were enclosed using ProLong Glass Antifade Mountant (P36965; Thermo Fisher Scientific) after staining the nuclei with 4′,6-diamidino-2phenylindole (DAPI; D523; Dojindo) at room temperature for 5 min. .. The fluorescent images were obtained under a fluorescence microscope (Keyence).

    Incubation:

    Article Title: Dysferlin promotes inflammatory macrophage responses and modulates Ca 2+ -dependent STAT1 signaling.
    Article Snippet: .. After washing with PBS, the cells and sections were incubated with an Alexa Fluor 488 Goat Anti-Rabbit IgG H&L (ab150077; Abcam), Alexa Fluor 488 Goat Anti-Mouse IgG H&L (ab150113; Abcam), Alexa Fluor 594 Goat Anti-Rabbit IgG H&L (ab150080; Abcam), or an Alexa 594 Anti-Mouse IgG antibody (ab150116; Abcam) as a secondary antibody at room temperature for 1 h. the cells and sections were enclosed using ProLong Glass Antifade Mountant (P36965; Thermo Fisher Scientific) after staining the nuclei with 4′,6-diamidino-2phenylindole (DAPI; D523; Dojindo) at room temperature for 5 min. .. The fluorescent images were obtained under a fluorescence microscope (Keyence).

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Blocking Assay:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Cell-Signaling:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.



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