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cellstack system  (Corning Life Sciences)

 
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    Corning Life Sciences cellstack system
    Cellstack System, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellstack+systems/cellstack/pmc08880717-356-19-21
    Average 90 stars, based on 1 article reviews
    cellstack system - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Small molecule induced STING degradation facilitated by the HECT ligase HERC4.
    Article Snippet: .. Briefly, 2.1 × 107 HEK293T cells were seeded into CellSTACK (Corning, Cat#3391) cell culture chambers and transfectedwith the sgRNA library plasmid mix together with ready-to-use lentiviral packaging plasmid mix (Cellecta, Cat#CPCP-K2A) 24 h after seeding using the Trans-IT transfection reagent (Mirus, Cat#MIR2304). .. Viral particles were harvested 72 h post-transfection and quantified using the Lenti-X qPCR kit (Clonetech, Cat#631235).

    Article Title: Limitations and challenges in the characterization of extracellular vesicles from stem cells and serum.
    Article Snippet: .. All donors have signed the informed consent forms. hUCESC cell culture and CM harvest hUCESC were cultured in cell stacks (Corning, NY) at 3000 cells/cm2 in DMEM-F12 with 10% fetal bovine serum (FBS), penicillin, and streptomycin (Corning) in an air-CO2 (95–5%) atmosphere at 37 °C in a Celsius 2007 cell incubator (Memmert GmbH, Germany). .. The cells were grown until 80% of cell confluence, cell culture media was discarded, and cells were washed three times with phosphate-buffered saline (PBS) (Lonza, Spain).

    Plasmid Preparation:

    Article Title: Small molecule induced STING degradation facilitated by the HECT ligase HERC4.
    Article Snippet: .. Briefly, 2.1 × 107 HEK293T cells were seeded into CellSTACK (Corning, Cat#3391) cell culture chambers and transfectedwith the sgRNA library plasmid mix together with ready-to-use lentiviral packaging plasmid mix (Cellecta, Cat#CPCP-K2A) 24 h after seeding using the Trans-IT transfection reagent (Mirus, Cat#MIR2304). .. Viral particles were harvested 72 h post-transfection and quantified using the Lenti-X qPCR kit (Clonetech, Cat#631235).

    Transfection:

    Article Title: Small molecule induced STING degradation facilitated by the HECT ligase HERC4.
    Article Snippet: .. Briefly, 2.1 × 107 HEK293T cells were seeded into CellSTACK (Corning, Cat#3391) cell culture chambers and transfectedwith the sgRNA library plasmid mix together with ready-to-use lentiviral packaging plasmid mix (Cellecta, Cat#CPCP-K2A) 24 h after seeding using the Trans-IT transfection reagent (Mirus, Cat#MIR2304). .. Viral particles were harvested 72 h post-transfection and quantified using the Lenti-X qPCR kit (Clonetech, Cat#631235).

    other:

    Article Title: Sabin inactivated polio vaccine upstream process development using fixed-bed bioreactor technology
    Article Snippet: For each experiment a cryo-vial of this Vero cell bank was thawed and expanded in T-flasks, followed by Cell Stacks (Corning), until sufficient viable cells were obtained to inoculate the bioreactor at 5000 cells/cm 2 in the 2.4 m 2 , 30 m 2 , and 150 m 2 scale-X fixed-bed bioreactors (Univercells Technologies).

    Article Title: Sabin inactivated polio vaccine upstream process development using fixed-bed bioreactor technology.
    Article Snippet: For each experiment a cryo-vial of this Vero cell bank was thawed and expanded in T-flasks, followed by Cell Stacks (Corning), until sufficient viable cells were obtained to inoculate the bioreactor at 5000 cells/cm2 in the 2.4 m2, 30 m2, and 150 m2 scale-X fixed-bed bioreactors (Univercells Technologies).

    Modification:

    Article Title: Comparability exercise of critical quality attributes of clinical-grade human mesenchymal stromal cells from the Wharton's jelly: single-use stirred tank bioreactors versus planar culture systems.
    Article Snippet: .. Each cryovial was thawed and plated onto 2-layer CellSTACK (Corning, Corning, NY, USA) at a cell density of 1.6 £ 103 2.1 £ 103 cells/cm2 using high-glucose (4.5 g/L) GlutaMAX Dulbecco’s Modified Eagle Medium (DMEM-HG; Gibco, Life Technologies, Waltham, MA, USA) and supplemented with 10% human serum B (hSerB; Banc de Sang i Teixits). ..

    Incubation:

    Article Title: Evaluation of Genotoxic Effects of N-Methyl-N-Nitroso-Urea and Etoposide on the Differentiation Potential of MSCs from Umbilical Cord Blood and Bone Marrow
    Article Snippet: .. Cells were expanded without dexamethasone in a closed system applying cell stacks (Corning), incubated at 37 °C in 5% CO 2 in a humidified atmosphere. .. After reaching 80% confluency, cells were detached with 0.25% trypsin (Lonza) and re-plated 1:3.

    Article Title: Evaluation of Genotoxic Effects of N-Methyl-N-Nitroso-Urea and Etoposide on the Differentiation Potential of MSCs from Umbilical Cord Blood and Bone Marrow
    Article Snippet: A total of 5–7 × 10 6 CB-MNC/mL was cultured in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Lonza, Basel, Switzerland) with 30% fetal calf serum (FCS) (Perbio, Bonn, Germany), 10 –7 M dexamethasone (Sigma-Aldrich, St-Louis, MO, USA), and penicillin/streptomycin (Lonza) until detection of adherent growing colonies. .. Cells were expanded without dexamethasone in a closed system applying cell stacks (Corning, New York, NY, USA), incubated at 37 °C in 5% CO 2 in a humidified atmosphere. .. After reaching 80% confluency, cells were detached with 0.25% trypsin (Lonza) and re-plated 1:3.



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    Optimizing the upstream lentivirus manufacturing process: HEK 293 or 293T cell lines were plated at a concentration of 1.25 × 105 cells/cm2 in 10-layer CellStack trays (a) or at 1 × 105 cells/cm2 in T-75 flasks (b–d). Two days later, cells were transfected with three helper plasmids (pCgp, pCMV-Rev2, and pCMV-G) and a <t>lentiviral</t> construct expressing EGFP in a ratio of 20:13:5:20 respectively with 0.8 µg DNA/cm2. After a three-hour incubation, transfection reagents were removed and replaced by fresh media containing 2% serum (a–d) or either 0%, 2%, or 10% serum (b) with (a–d) or without (c) sodium butyrate. Crude supernatant was harvested 72 hours later (a–c) or either two, three, or four days later (d). For flasks with supernatant removed on day 2, an equivalent volume of media was added back to incubate until day 3 (sample day 2–3) (d). Supernatant from each sample was used to transduce HT-1080 cells, and GFP expression was measured by FACS analysis to assess viral titer. Panel a shows the mean ± standard deviation (SD) of four experiments; panels b–d show the mean ± SD of three experiments.
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    Optimizing the upstream lentivirus manufacturing process: HEK 293 or 293T cell lines were plated at a concentration of 1.25 × 105 cells/cm2 in 10-layer CellStack trays (a) or at 1 × 105 cells/cm2 in T-75 flasks (b–d). Two days later, cells were transfected with three helper plasmids (pCgp, pCMV-Rev2, and pCMV-G) and a lentiviral construct expressing EGFP in a ratio of 20:13:5:20 respectively with 0.8 µg DNA/cm2. After a three-hour incubation, transfection reagents were removed and replaced by fresh media containing 2% serum (a–d) or either 0%, 2%, or 10% serum (b) with (a–d) or without (c) sodium butyrate. Crude supernatant was harvested 72 hours later (a–c) or either two, three, or four days later (d). For flasks with supernatant removed on day 2, an equivalent volume of media was added back to incubate until day 3 (sample day 2–3) (d). Supernatant from each sample was used to transduce HT-1080 cells, and GFP expression was measured by FACS analysis to assess viral titer. Panel a shows the mean ± standard deviation (SD) of four experiments; panels b–d show the mean ± SD of three experiments.

    Journal: BioProcess international

    Article Title: Production of CGMP-Grade Lentiviral Vectors

    doi:

    Figure Lengend Snippet: Optimizing the upstream lentivirus manufacturing process: HEK 293 or 293T cell lines were plated at a concentration of 1.25 × 105 cells/cm2 in 10-layer CellStack trays (a) or at 1 × 105 cells/cm2 in T-75 flasks (b–d). Two days later, cells were transfected with three helper plasmids (pCgp, pCMV-Rev2, and pCMV-G) and a lentiviral construct expressing EGFP in a ratio of 20:13:5:20 respectively with 0.8 µg DNA/cm2. After a three-hour incubation, transfection reagents were removed and replaced by fresh media containing 2% serum (a–d) or either 0%, 2%, or 10% serum (b) with (a–d) or without (c) sodium butyrate. Crude supernatant was harvested 72 hours later (a–c) or either two, three, or four days later (d). For flasks with supernatant removed on day 2, an equivalent volume of media was added back to incubate until day 3 (sample day 2–3) (d). Supernatant from each sample was used to transduce HT-1080 cells, and GFP expression was measured by FACS analysis to assess viral titer. Panel a shows the mean ± standard deviation (SD) of four experiments; panels b–d show the mean ± SD of three experiments.

    Article Snippet: Open in a separate window Figure 5 A semiclosed system for transfection of GMP -grade lentiviral product; Corning CellStack systems, media bags, transfection bottles, and the other components are parts of a manifold connected by disposable tubing and connectors.

    Techniques: Concentration Assay, Transfection, Construct, Expressing, Incubation, Transduction, Standard Deviation

    A semiclosed system for transfection of GMP -grade lentiviral product; Corning CellStack systems, media bags, transfection bottles, and the other components are parts of a manifold connected by disposable tubing and connectors. Following formation of DNA precipitates, existing media are removed by connecting the 10-layer trays to a manifold for transfection (a). The transfection medium is removed after incubation by a peristaltic pump using a second tubing manifold (b). On day 3 after transfection, supernatant containing secreted lentiviral vectors is collected by a third manifold (c). Sterile samples of crude supernatant and cells are collected for QC assays.

    Journal: BioProcess international

    Article Title: Production of CGMP-Grade Lentiviral Vectors

    doi:

    Figure Lengend Snippet: A semiclosed system for transfection of GMP -grade lentiviral product; Corning CellStack systems, media bags, transfection bottles, and the other components are parts of a manifold connected by disposable tubing and connectors. Following formation of DNA precipitates, existing media are removed by connecting the 10-layer trays to a manifold for transfection (a). The transfection medium is removed after incubation by a peristaltic pump using a second tubing manifold (b). On day 3 after transfection, supernatant containing secreted lentiviral vectors is collected by a third manifold (c). Sterile samples of crude supernatant and cells are collected for QC assays.

    Article Snippet: Open in a separate window Figure 5 A semiclosed system for transfection of GMP -grade lentiviral product; Corning CellStack systems, media bags, transfection bottles, and the other components are parts of a manifold connected by disposable tubing and connectors.

    Techniques: Transfection, Incubation, Sterility

    Size analysis of residual host DNA present in a CGMP lentiviral product performed as described in the text

    Journal: BioProcess international

    Article Title: Production of CGMP-Grade Lentiviral Vectors

    doi:

    Figure Lengend Snippet: Size analysis of residual host DNA present in a CGMP lentiviral product performed as described in the text

    Article Snippet: Open in a separate window Figure 5 A semiclosed system for transfection of GMP -grade lentiviral product; Corning CellStack systems, media bags, transfection bottles, and the other components are parts of a manifold connected by disposable tubing and connectors.

    Techniques: