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normalization kit  (Dojindo Labs)


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    Structured Review

    Dojindo Labs normalization kit
    Normalization Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+count+normalization+kit/Cell+Count+Normalization+Kit/pm40204187-91-72-75
    Average 93 stars, based on 32 article reviews
    normalization kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cell Counting:

    Article Title: Leucine-rich repeat kinase 2 promotes disintegration of retinal pigment epithelial cell: Implication in the pathogenesis of dry age-related macular degeneration.
    Article Snippet: Page 11 of 34 Subsequently, 20 μg/mL of recombinant human α-synuclein fibrils (SPR-322, 1 StressMarq Biosciences Inc.) were added and incubated for 24, 48, and 72 h. 2 Caspase-3/7 activity was measured using caspase-Glo 3/7 Assay (Promega) in 3 accordance with the manufacturer’s instructions. .. 4 5 Cell counting 6 hTERT-RPE1 cells were seeded into clear-bottom 96-well plates at a density of 7 1.5×103 cells/well and incubated for 24 h. Subsequently, 20 μg/mL of recombinant 8 human α-synuclein fibrils (SPR-322, StressMarq Biosciences Inc., Victoria, Canada) 9 and control (PBS buffer) were added and incubated for 24, 48, and 72 h. Cell nuclei 10 were stained with a nuclear staining reagent (Hoechst 33342) using a Cell Count 11 Normalization Kit (#C544; Dojindo, Kumamoto, Japan). .. Three locations per well were 12 photographed, the number of cells in each photograph was counted using the image 13 analysis software ImageJ (https://imagej.net/software/imagej/, version: 1.53t, date of 14 last access: September 10, 2022), and the average value was calculated.

    Incubation:

    Article Title: Leucine-rich repeat kinase 2 promotes disintegration of retinal pigment epithelial cell: Implication in the pathogenesis of dry age-related macular degeneration.
    Article Snippet: Page 11 of 34 Subsequently, 20 μg/mL of recombinant human α-synuclein fibrils (SPR-322, 1 StressMarq Biosciences Inc.) were added and incubated for 24, 48, and 72 h. 2 Caspase-3/7 activity was measured using caspase-Glo 3/7 Assay (Promega) in 3 accordance with the manufacturer’s instructions. .. 4 5 Cell counting 6 hTERT-RPE1 cells were seeded into clear-bottom 96-well plates at a density of 7 1.5×103 cells/well and incubated for 24 h. Subsequently, 20 μg/mL of recombinant 8 human α-synuclein fibrils (SPR-322, StressMarq Biosciences Inc., Victoria, Canada) 9 and control (PBS buffer) were added and incubated for 24, 48, and 72 h. Cell nuclei 10 were stained with a nuclear staining reagent (Hoechst 33342) using a Cell Count 11 Normalization Kit (#C544; Dojindo, Kumamoto, Japan). .. Three locations per well were 12 photographed, the number of cells in each photograph was counted using the image 13 analysis software ImageJ (https://imagej.net/software/imagej/, version: 1.53t, date of 14 last access: September 10, 2022), and the average value was calculated.

    Recombinant:

    Article Title: Leucine-rich repeat kinase 2 promotes disintegration of retinal pigment epithelial cell: Implication in the pathogenesis of dry age-related macular degeneration.
    Article Snippet: Page 11 of 34 Subsequently, 20 μg/mL of recombinant human α-synuclein fibrils (SPR-322, 1 StressMarq Biosciences Inc.) were added and incubated for 24, 48, and 72 h. 2 Caspase-3/7 activity was measured using caspase-Glo 3/7 Assay (Promega) in 3 accordance with the manufacturer’s instructions. .. 4 5 Cell counting 6 hTERT-RPE1 cells were seeded into clear-bottom 96-well plates at a density of 7 1.5×103 cells/well and incubated for 24 h. Subsequently, 20 μg/mL of recombinant 8 human α-synuclein fibrils (SPR-322, StressMarq Biosciences Inc., Victoria, Canada) 9 and control (PBS buffer) were added and incubated for 24, 48, and 72 h. Cell nuclei 10 were stained with a nuclear staining reagent (Hoechst 33342) using a Cell Count 11 Normalization Kit (#C544; Dojindo, Kumamoto, Japan). .. Three locations per well were 12 photographed, the number of cells in each photograph was counted using the image 13 analysis software ImageJ (https://imagej.net/software/imagej/, version: 1.53t, date of 14 last access: September 10, 2022), and the average value was calculated.

    SPR Assay:

    Article Title: Leucine-rich repeat kinase 2 promotes disintegration of retinal pigment epithelial cell: Implication in the pathogenesis of dry age-related macular degeneration.
    Article Snippet: Page 11 of 34 Subsequently, 20 μg/mL of recombinant human α-synuclein fibrils (SPR-322, 1 StressMarq Biosciences Inc.) were added and incubated for 24, 48, and 72 h. 2 Caspase-3/7 activity was measured using caspase-Glo 3/7 Assay (Promega) in 3 accordance with the manufacturer’s instructions. .. 4 5 Cell counting 6 hTERT-RPE1 cells were seeded into clear-bottom 96-well plates at a density of 7 1.5×103 cells/well and incubated for 24 h. Subsequently, 20 μg/mL of recombinant 8 human α-synuclein fibrils (SPR-322, StressMarq Biosciences Inc., Victoria, Canada) 9 and control (PBS buffer) were added and incubated for 24, 48, and 72 h. Cell nuclei 10 were stained with a nuclear staining reagent (Hoechst 33342) using a Cell Count 11 Normalization Kit (#C544; Dojindo, Kumamoto, Japan). .. Three locations per well were 12 photographed, the number of cells in each photograph was counted using the image 13 analysis software ImageJ (https://imagej.net/software/imagej/, version: 1.53t, date of 14 last access: September 10, 2022), and the average value was calculated.

    Control:

    Article Title: Leucine-rich repeat kinase 2 promotes disintegration of retinal pigment epithelial cell: Implication in the pathogenesis of dry age-related macular degeneration.
    Article Snippet: Page 11 of 34 Subsequently, 20 μg/mL of recombinant human α-synuclein fibrils (SPR-322, 1 StressMarq Biosciences Inc.) were added and incubated for 24, 48, and 72 h. 2 Caspase-3/7 activity was measured using caspase-Glo 3/7 Assay (Promega) in 3 accordance with the manufacturer’s instructions. .. 4 5 Cell counting 6 hTERT-RPE1 cells were seeded into clear-bottom 96-well plates at a density of 7 1.5×103 cells/well and incubated for 24 h. Subsequently, 20 μg/mL of recombinant 8 human α-synuclein fibrils (SPR-322, StressMarq Biosciences Inc., Victoria, Canada) 9 and control (PBS buffer) were added and incubated for 24, 48, and 72 h. Cell nuclei 10 were stained with a nuclear staining reagent (Hoechst 33342) using a Cell Count 11 Normalization Kit (#C544; Dojindo, Kumamoto, Japan). .. Three locations per well were 12 photographed, the number of cells in each photograph was counted using the image 13 analysis software ImageJ (https://imagej.net/software/imagej/, version: 1.53t, date of 14 last access: September 10, 2022), and the average value was calculated.

    Staining:

    Article Title: Leucine-rich repeat kinase 2 promotes disintegration of retinal pigment epithelial cell: Implication in the pathogenesis of dry age-related macular degeneration.
    Article Snippet: Page 11 of 34 Subsequently, 20 μg/mL of recombinant human α-synuclein fibrils (SPR-322, 1 StressMarq Biosciences Inc.) were added and incubated for 24, 48, and 72 h. 2 Caspase-3/7 activity was measured using caspase-Glo 3/7 Assay (Promega) in 3 accordance with the manufacturer’s instructions. .. 4 5 Cell counting 6 hTERT-RPE1 cells were seeded into clear-bottom 96-well plates at a density of 7 1.5×103 cells/well and incubated for 24 h. Subsequently, 20 μg/mL of recombinant 8 human α-synuclein fibrils (SPR-322, StressMarq Biosciences Inc., Victoria, Canada) 9 and control (PBS buffer) were added and incubated for 24, 48, and 72 h. Cell nuclei 10 were stained with a nuclear staining reagent (Hoechst 33342) using a Cell Count 11 Normalization Kit (#C544; Dojindo, Kumamoto, Japan). .. Three locations per well were 12 photographed, the number of cells in each photograph was counted using the image 13 analysis software ImageJ (https://imagej.net/software/imagej/, version: 1.53t, date of 14 last access: September 10, 2022), and the average value was calculated.

    Cell Characterization:

    Article Title: Leucine-rich repeat kinase 2 promotes disintegration of retinal pigment epithelial cell: Implication in the pathogenesis of dry age-related macular degeneration.
    Article Snippet: Page 11 of 34 Subsequently, 20 μg/mL of recombinant human α-synuclein fibrils (SPR-322, 1 StressMarq Biosciences Inc.) were added and incubated for 24, 48, and 72 h. 2 Caspase-3/7 activity was measured using caspase-Glo 3/7 Assay (Promega) in 3 accordance with the manufacturer’s instructions. .. 4 5 Cell counting 6 hTERT-RPE1 cells were seeded into clear-bottom 96-well plates at a density of 7 1.5×103 cells/well and incubated for 24 h. Subsequently, 20 μg/mL of recombinant 8 human α-synuclein fibrils (SPR-322, StressMarq Biosciences Inc., Victoria, Canada) 9 and control (PBS buffer) were added and incubated for 24, 48, and 72 h. Cell nuclei 10 were stained with a nuclear staining reagent (Hoechst 33342) using a Cell Count 11 Normalization Kit (#C544; Dojindo, Kumamoto, Japan). .. Three locations per well were 12 photographed, the number of cells in each photograph was counted using the image 13 analysis software ImageJ (https://imagej.net/software/imagej/, version: 1.53t, date of 14 last access: September 10, 2022), and the average value was calculated.

    Article Title: Carnosol, a Rosemary Ingredient Discovered in a Screen for Inhibitors of SARM1-NAD + Cleavage Activity, Ameliorates Symptoms of Peripheral Neuropathy.
    Article Snippet: Luminescence was observed using a Fluoroskan Ascent FL plate reader (Thermo Fisher Scientific). .. To analyze intracellular NAD+ levels, a Cell Count Normalization Kit (Dojindo, Kumamoto, Japan) was used to normalize cell numbers before the NAD+ assay. .. The fluorescence intensity of cells stained with Hoechst 33342 was detected by using a FlexStation 3 microplate reader (Molecular Devices, Sunnyvale, CA, USA; Ex: 350 nm; Em: 461 nm).

    Article Title: Influence of Hepatocellular Senescence on Serum Amyloid A Expression and Response to Proinflammatory Cytokines under Steatotic Conditions.
    Article Snippet: .. The absorbance of Oil Red O was normalized for fluctuations in cell number across wells using the Cell Count Normalization Kit (Dojindo), which estimates cell number based on Hoechst fluorescence. .. Cytokine Treatment Recombinant human interleukin-1β (IL-1β) and IL-6 were purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Carnosol, a Rosemary Ingredient Discovered in a Screen for Inhibitors of SARM1-NAD + Cleavage Activity, Ameliorates Symptoms of Peripheral Neuropathy
    Article Snippet: Luminescence was observed using a Fluoroskan Ascent FL plate reader (Thermo Fisher Scientific). .. To analyze intracellular NAD + levels, a Cell Count Normalization Kit (Dojindo, Kumamoto, Japan) was used to normalize cell numbers before the NAD + assay. .. The fluorescence intensity of cells stained with Hoechst 33342 was detected by using a FlexStation 3 microplate reader (Molecular Devices, Sunnyvale, CA, USA; Ex: 350 nm; Em: 461 nm).

    Article Title: A human and mouse subpopulation of senescent β-cells induces pathologic dysfunction through targetable paracrine signaling
    Article Snippet: Following Xenium, multiplexed antibody-based immunofluorescence staining and imaging was performed using the Phenocycler-Fusion (Akoya Biosciences) according to a custom protocol ( https://dx.doi.org/10.17504/protocols.io.q26g71rwqgwz/v1 )]. .. To measure senescence-associated β-galactosidase (SA-βGal) activity, we used the Dojindo Cell Count Normalization Kit (C544) combined with the Cellular Senescence Plate Assay Kit – Spider-βGal (SG05) following the manufacturer’s protocol for combined analysis. ..

    Article Title: A novel senotherapeutic strategy with azithromycin for preventing endometriosis progression.
    Article Snippet: SA-β-Gal activity was quantitated with a Cellular Senescence Plate Assay Kit using SPiDER-βGal, a fluorogenic substrate for β-galactosidase (SG05, Dojindo, Kumamoto, Japan), following the manufacturer’s instructions. .. The optimal cell numbers in each well were adjusted using a Cell Count Normalization Kit (C544, Dojindo, Kumamoto, Japan), following the manufacturer’s instructions. .. Cells were lysed with Lysis Buffer for 10 min at room temperature, then incubated with SPiDER-βGal working solution at 37 °C for 30 min. After adding a stop solution, using an excitation wavelength of 535 nm, emission at 580 nm was recorded using a multimode microplate reader (SpectraMax iD5, Molecular Devices, San Jose, CA, USA).

    Fluorescence:

    Article Title: Influence of Hepatocellular Senescence on Serum Amyloid A Expression and Response to Proinflammatory Cytokines under Steatotic Conditions.
    Article Snippet: .. The absorbance of Oil Red O was normalized for fluctuations in cell number across wells using the Cell Count Normalization Kit (Dojindo), which estimates cell number based on Hoechst fluorescence. .. Cytokine Treatment Recombinant human interleukin-1β (IL-1β) and IL-6 were purchased from FUJIFILM Wako Pure Chemical Corporation.

    Activity Assay:

    Article Title: A human and mouse subpopulation of senescent β-cells induces pathologic dysfunction through targetable paracrine signaling
    Article Snippet: Following Xenium, multiplexed antibody-based immunofluorescence staining and imaging was performed using the Phenocycler-Fusion (Akoya Biosciences) according to a custom protocol ( https://dx.doi.org/10.17504/protocols.io.q26g71rwqgwz/v1 )]. .. To measure senescence-associated β-galactosidase (SA-βGal) activity, we used the Dojindo Cell Count Normalization Kit (C544) combined with the Cellular Senescence Plate Assay Kit – Spider-βGal (SG05) following the manufacturer’s protocol for combined analysis. ..



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