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overrepresentation analysis table s3d
Overrepresentation Analysis Table S3d, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cebpd/CEBPD+Human+siRNA+Oligo+Duplex/pm35905723-357-26-35
Average 90 stars, based on 1 article reviews
Overrepresentation Analysis Table S3d, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cebpd/CEBPD+Human+siRNA+Oligo+Duplex/pm35905723-357-26-35
Average 90 stars, based on 1 article reviews
overrepresentation analysis table s3d - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution."
Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution.
Journal: Cell reports
doi: 10.1016/j.celrep.2022.111136
Figure Legend Snippet: Figure 5. HOTAIR-PRC2 regulates developmental pathways in differentiating gluteal preadipocytes (A) Venn diagram showing overlap between PRC2 target genes (from FUMA and CHEA) and shHOTAIR WGCNA DEGs (upregulated at all time points, light-green module). See also Figure S6. (B) qPCR confirmation of PCDH10 expression in shHOTAIR and shControl cells during adipogenesis (n = 3). Data were assessed using 2-way ANOVA; *p < 0.05. (C and D) ChIP to assess (C) SUZ12 and (D) H3K27me3 enrichment at the PCDH10 promoter in gluteal shHOTAIR and shControl cells on differentiation day 0 (n = 3). Mouse IgG antibody was used as a negative control. Data were assessed by Student’s t test; *p < 0.05. (E) Expression heatmap of shHOTAIR DEGs annotated to UniProtKB: Wnt signaling (shHOTAIR versus shControl; rlog normalized fold changes DESeq2). (F) HOTAIR1 gene expression in imGSAT cells expressing the 7TFP TOPflash luciferase vector following 72 h HOTAIR siRNA treatment (n = 3, paired t test). (G) TOPflash promoter activity in Control and siHOTAIR imGSAT cells (n = 5, paired t test). (H) Adipogenesis assessed by AdipoRed staining in control and siHOTAIR cells treated with CHIR99021 1 mM and 3 mM throughout adipogenic differentiation (n = 3, ANOVA). (I) Differential alternative splicing (DAS) events between shControl and shHOTAIR cells determined by SUPPA2:diffSplice. Alternative first (AF) or last (AL) exon, alternative 50 or 30 splice site (A5 and A3), mutually exclusive (MX) and skipped exons (SE), and retained introns (RI). (J) Number of genes that are differentially alternatively spliced at both the isoform (limma) and event (SUPPA2) level across each day of differentiation. (K) The proportion splice-in (PSI) of significantly expressed PPARG (GENCODE) isoforms in shControl and shHOTAIR cells across each day of differentiation. *p < 0.05 (shControl versus shHOTAIR). Data presented as means ± SEMs (B–D and F–H). See also Figure S7. n represents the number of experimental replicates.
Techniques Used: Expressing, Negative Control, Gene Expression, Luciferase, Plasmid Preparation, Activity Assay, Control, Staining, Alternative Splicing
Related Articles
Expressing:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Negative Control:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Gene Expression:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Luciferase:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Plasmid Preparation:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Activity Assay:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Control:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Staining:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Alternative Splicing:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Recombinant:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: DC Protein Assay:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: shRNA:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Western Blot:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: Software:Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution. Article Snippet: SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).SUPPA2 (Trincado et al., 2018) was used to survey event-level DAS, specifying a –lower-bound change in PSI >0.1 (Table S3C).. Lists of DAS genes were then uploaded to the gProfiler online g:GOSt tool (Raudvere et al., 2019) for overrepresentation analysis (Table S3D). siRNA rescue experiments Human siRNA oligo duplexes were used to transiently suppress CEBPD ( Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: for custom designed Taqman assays for gene promoter regions , Bio-Rad ThermoFisher Scientific , N/A. .. siRNA CEBPD , Article Title: HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution Article Snippet: |
