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hiscript ii 1st strand cdna synthesis kit  (Vazyme Biotech Co)


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  • 97

    Structured Review

    Vazyme Biotech Co hiscript ii 1st strand cdna synthesis kit
    Hiscript Ii 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+reverse+transcriptase+kit/HiScript+II+Reverse+Transcriptase/pmc12993239-123-11-18
    Average 97 stars, based on 1476 article reviews
    hiscript ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    Reverse Transcription:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    Article Title: Transcriptomic and metabolomic insights into high-temperature acclimation mechanisms of Anoectochilus roxburghii
    Article Snippet: .. Reverse transcription of the plant RNA samples was conducted using a HiScript Reverse Transcriptase kit from Vazyme (China). qPCR was performed on a LightCycler 96 instrument (Roche, USA) utilizing the ChamQ qPCR Mix from Vazyme. ..

    Article Title: Lactic acid promotes metastasis of papillary thyroid carcinoma by enhancing CPT1A lactylation.
    Article Snippet: .. Total RNA was isolated using an RNA extraction kit (ESScience, RN001). cDNA synthesis was performed with HiScript II Reverse Transcriptase (Vazyme, RL201-01). .. RT-qPCR was conducted using ChamQ SYBR Master Mix (Vazyme, Q711-02) on a Bio-Rad CFX96 system.

    Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library
    Article Snippet: Total RNA was extracted using a Bacterial RNA Kit (Omega Bio-tek). .. For quantitative reverse transcription PCR (RT-qPCR), 1 μg of RNA was converted to complementary DNA (cDNA) using HiScript II Reverse Transcriptase (Vazyme) in 20 μl reactions. qPCR was performed using the aforementioned method with 0.5 ng of cDNA as a template. gapA was used for normalization. ..

    Article Title: CircNOLC1/KRT16 promotes colorectal cancer invasion and migration via c-met nuclear translocation and reprogramming of the oxidative pentose phosphate pathway
    Article Snippet: .. Total RNA was extracted from cells using the Trizol reagent kit (Accurate Biology), and reverse transcription was performed using HiScript II reverse transcriptase (Vazyme Biotech). ..

    Article Title: The Transcription Factor SOX6 Regulates Autophagy Levels Through LAMP5 to Inhibit the Development of Pituitary Adenomas.
    Article Snippet: Pituitary adenoma (PA) is a common type of brain tumor, and existing treatment options have limited efficacy in clinical settings.. Autophagy is a risk factor for PA progression, and lysosome-associated membrane protein family member 5 (LAMP5) plays a regulatory role in tumor autophagy.. Therefore, this study aimed to investigate the role of LAMP5 in PA autophagy, laying the foundation for the development of alternative therapies for PA. A tumor-bearing model was constructed by injecting rat pituitary tumor cells (GH3) into the left back of nude mice.

    Quantitative RT-PCR:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    SYBR Green Assay:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    Control:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptomic and metabolomic insights into high-temperature acclimation mechanisms of Anoectochilus roxburghii
    Article Snippet: .. Reverse transcription of the plant RNA samples was conducted using a HiScript Reverse Transcriptase kit from Vazyme (China). qPCR was performed on a LightCycler 96 instrument (Roche, USA) utilizing the ChamQ qPCR Mix from Vazyme. ..

    Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library
    Article Snippet: Total RNA was extracted using a Bacterial RNA Kit (Omega Bio-tek). .. For quantitative reverse transcription PCR (RT-qPCR), 1 μg of RNA was converted to complementary DNA (cDNA) using HiScript II Reverse Transcriptase (Vazyme) in 20 μl reactions. qPCR was performed using the aforementioned method with 0.5 ng of cDNA as a template. gapA was used for normalization. ..

    Isolation:

    Article Title: Lactic acid promotes metastasis of papillary thyroid carcinoma by enhancing CPT1A lactylation.
    Article Snippet: .. Total RNA was isolated using an RNA extraction kit (ESScience, RN001). cDNA synthesis was performed with HiScript II Reverse Transcriptase (Vazyme, RL201-01). .. RT-qPCR was conducted using ChamQ SYBR Master Mix (Vazyme, Q711-02) on a Bio-Rad CFX96 system.

    RNA Extraction:

    Article Title: Lactic acid promotes metastasis of papillary thyroid carcinoma by enhancing CPT1A lactylation.
    Article Snippet: .. Total RNA was isolated using an RNA extraction kit (ESScience, RN001). cDNA synthesis was performed with HiScript II Reverse Transcriptase (Vazyme, RL201-01). .. RT-qPCR was conducted using ChamQ SYBR Master Mix (Vazyme, Q711-02) on a Bio-Rad CFX96 system.

    cDNA Synthesis:

    Article Title: Lactic acid promotes metastasis of papillary thyroid carcinoma by enhancing CPT1A lactylation.
    Article Snippet: .. Total RNA was isolated using an RNA extraction kit (ESScience, RN001). cDNA synthesis was performed with HiScript II Reverse Transcriptase (Vazyme, RL201-01). .. RT-qPCR was conducted using ChamQ SYBR Master Mix (Vazyme, Q711-02) on a Bio-Rad CFX96 system.

    Polymerase Chain Reaction:

    Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library
    Article Snippet: Total RNA was extracted using a Bacterial RNA Kit (Omega Bio-tek). .. For quantitative reverse transcription PCR (RT-qPCR), 1 μg of RNA was converted to complementary DNA (cDNA) using HiScript II Reverse Transcriptase (Vazyme) in 20 μl reactions. qPCR was performed using the aforementioned method with 0.5 ng of cDNA as a template. gapA was used for normalization. ..



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