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human cdna microarray slides  (MicroMax Inc)

 
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    MicroMax Inc human cdna microarray slides
    Human Cdna Microarray Slides, supplied by MicroMax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+slide/cdna+microarray+system/pmc03991976-2-19-18
    Average 90 stars, based on 1 article reviews
    human cdna microarray slides - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Evaluation of local and circulating osteopontin in malignant and benign primary bone tumors
    Article Snippet: Furthermore, using the MICROMAX complementary DNA (cDNA) microarray system, Wong et al. (2001) identified 30 putative genes, including OPN, that are differentially overexpressed in ovarian cancer cell lines .

    Article Title: A New Bioinformatics Approach to Analyze Gene Expressions and Signaling Pathways Reveals Unique Purine Gene Dysregulation Profiles that Distinguish between CD and UC
    Article Snippet: For other genes, unrelated to purines, global cDNA microarray analysis or restricted cDNA array (MICROMAX) in mucosal biopsies or PBMCs revealed a multitude of genes that become dysregulated in human IBD, and provided evidence for significant differences in gene dysregulation profiles between CD and UC.

    Article Title: Beyond CA125: the coming of age of ovarian cancer biomarkers. Are we there yet?
    Article Snippet: SPP1 (Ostepontin, secreted phosphoprotein 1) was initially described as a potential biomarker for ovarian cancer in a MICROMAX cDNA microarray study using RNA isolated from several ovarian cancer cell lines and healthy human ovarian surface epithelial (HOSE) cell cultures [ 84 ].

    Article Title: Cancer-Associated Fibroblasts: Epigenetic Regulation and Therapeutic Intervention in Breast Cancer
    Article Snippet: In a comparison study of gene expression between BCAFs and NFs, the MICROMAX TM human cDNA microarray system was selected by Singer and colleagues.

    Microarray:

    Article Title: Differential Expression of Genes in Cells Cultured from Juxtacanalicular Trabecular Meshwork and Schlemm's Canal
    Article Snippet: .. The cells were thawed, total RNA was extracted, and the probes made from total RNAs were hybridized to MICROMAX human cDNA microarray slides in 2 separate trials. ..

    Article Title: Amplified array analysis system
    Article Snippet: The cyanine 5 labeled cDNA was hybridized to Practice Slides (MICROMAX Human cDNA Microarray System I, NEN Life Science Products, Boston, Mass.) according to MICROMAX Human cDNA System I-Direct (NEN Life Science Products, Boston, Mass.) kit directions. .. For amplified analysis, biotin labeled cDNA was prepared from 4 μg Jurkat total RNA using the MICROMAX Human cDNA Microarray System I kit reagents and protocols. .. Hybridization to Practice Slides and amplified detection using streptavidin-HRP and cyanine 5 tyramide were according to the MICROMAX Human cDNA Microarray System I kit directions.

    Article Title: Genomic and proteomic profiling of oxidative stress response in human diploid fibroblasts
    Article Snippet: Briefly, total RNAs were isolated using a QIAGEN RNeasy kit (Qiagen, Valencia, CA). .. Cy3 or Cy5 labeled first strand cDNAs were made from 40 μg of total RNA with Micromax Direct cDNA Microarray System (NEN Life Sciences, Boston, MA). .. Fluorescence labeled cDNAs from two reactions were hybridized to the cDNA array slides for scanning with Axon GenePix 4000 microarray reader (Axon Instruments, Foster City, CA) and quantification with GenePix software.

    Amplification:

    Article Title: Amplified array analysis system
    Article Snippet: The cyanine 5 labeled cDNA was hybridized to Practice Slides (MICROMAX Human cDNA Microarray System I, NEN Life Science Products, Boston, Mass.) according to MICROMAX Human cDNA System I-Direct (NEN Life Science Products, Boston, Mass.) kit directions. .. For amplified analysis, biotin labeled cDNA was prepared from 4 μg Jurkat total RNA using the MICROMAX Human cDNA Microarray System I kit reagents and protocols. .. Hybridization to Practice Slides and amplified detection using streptavidin-HRP and cyanine 5 tyramide were according to the MICROMAX Human cDNA Microarray System I kit directions.

    Labeling:

    Article Title: Amplified array analysis system
    Article Snippet: The cyanine 5 labeled cDNA was hybridized to Practice Slides (MICROMAX Human cDNA Microarray System I, NEN Life Science Products, Boston, Mass.) according to MICROMAX Human cDNA System I-Direct (NEN Life Science Products, Boston, Mass.) kit directions. .. For amplified analysis, biotin labeled cDNA was prepared from 4 μg Jurkat total RNA using the MICROMAX Human cDNA Microarray System I kit reagents and protocols. .. Hybridization to Practice Slides and amplified detection using streptavidin-HRP and cyanine 5 tyramide were according to the MICROMAX Human cDNA Microarray System I kit directions.

    Article Title: Genomic and proteomic profiling of oxidative stress response in human diploid fibroblasts
    Article Snippet: Briefly, total RNAs were isolated using a QIAGEN RNeasy kit (Qiagen, Valencia, CA). .. Cy3 or Cy5 labeled first strand cDNAs were made from 40 μg of total RNA with Micromax Direct cDNA Microarray System (NEN Life Sciences, Boston, MA). .. Fluorescence labeled cDNAs from two reactions were hybridized to the cDNA array slides for scanning with Axon GenePix 4000 microarray reader (Axon Instruments, Foster City, CA) and quantification with GenePix software.

    Control:

    Article Title: Internal standard-based analysis of microarray data2—Analysis of functional associations between HVE-genes
    Article Snippet: 1 , JRA , J Jarvis, OUHSC, OK , D. Peripheral blood of Patients 3–15 years (21 samples) and healthy control donors (19 samples) , Human WG-6 v3.0 beadchip (Illumina, San Diego, CA, USA). .. . Peripheral blood of Patients 3–15 years (15 samples) and healthy control donors (12 samples) , Micromax cDNA arrays, Perkin Elmer Life Sci., Boston, MA, USA. .. 2 , Chronic Lymphocyte Leukemia (CLL) , N Chiorazzi, Feinstein Inst. Med. Res., NY , C. B cells from peripheral blood of CLL patients (20-with mutated IGHV, and 16 with unmutated IGHV) and of 18 healthy control donors , Human WG-6 v3.0 beadchip (Illumina, San Diego, CA, USA).



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    Image Search Results


    Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Journal: Scientific Reports

    Article Title: Extracellular matrix stiffness dictates Wnt expression through integrin pathway

    doi: 10.1038/srep20395

    Figure Lengend Snippet: Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Article Snippet: Microarray analyses were performed using commercial Mouse cDNA Microarray slides (Phalanx Biotech Group; Hsinchu, Taiwan) according to the manufacturer’s instructions.

    Techniques: Microarray, Software, Western Blot, In Situ, Fluorescence, Staining