mca1424 (Bio-Rad)
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mca1424
Mca1424, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd21/Mouse+anti+Bovine+CD21/us07420099-945-4-12
Average 94 stars, based on 79 article reviews
Mca1424, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd21/Mouse+anti+Bovine+CD21/us07420099-945-4-12
Average 94 stars, based on 79 article reviews
mca1424 - by Bioz Stars,
2026-10
94/100 stars
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Saline:Article Title: Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves Article Snippet: Blood samples (100 μL) were incubated with VersaLyse (Beckman Coulter GmbH, Krefeld, Germany) for 20 min at room temperature and centrifuged at 250 × g for 5 min. .. Obtained cell pellets were washed twice by adding 700 μL phosphate-buffered saline (PBS) and subsequent centrifugation at 150 × g for 5 min. Staining of leukocytes was reached by adding monoclonal antibodies for marking CD4 (co-receptor primarily expressed on T-helper cells, monocytes, and macrophages; mouse anti-bovine CD4: +FITC; Bio-Rad, Hercules, USA), CD8 (co-receptor primarily expressed on cytotoxic T cells; mouse anti-bovine CD8: +RPE; Bio-Rad), CD14 (receptor expressed on monocytes and macrophages; mouse anti-bovine CD14: +RPE; Bio-Rad), and Centrifugation:Article Title: Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves Article Snippet: Blood samples (100 μL) were incubated with VersaLyse (Beckman Coulter GmbH, Krefeld, Germany) for 20 min at room temperature and centrifuged at 250 × g for 5 min. .. Obtained cell pellets were washed twice by adding 700 μL phosphate-buffered saline (PBS) and subsequent centrifugation at 150 × g for 5 min. Staining of leukocytes was reached by adding monoclonal antibodies for marking CD4 (co-receptor primarily expressed on T-helper cells, monocytes, and macrophages; mouse anti-bovine CD4: +FITC; Bio-Rad, Hercules, USA), CD8 (co-receptor primarily expressed on cytotoxic T cells; mouse anti-bovine CD8: +RPE; Bio-Rad), CD14 (receptor expressed on monocytes and macrophages; mouse anti-bovine CD14: +RPE; Bio-Rad), and Staining:Article Title: Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves Article Snippet: Blood samples (100 μL) were incubated with VersaLyse (Beckman Coulter GmbH, Krefeld, Germany) for 20 min at room temperature and centrifuged at 250 × g for 5 min. .. Obtained cell pellets were washed twice by adding 700 μL phosphate-buffered saline (PBS) and subsequent centrifugation at 150 × g for 5 min. Staining of leukocytes was reached by adding monoclonal antibodies for marking CD4 (co-receptor primarily expressed on T-helper cells, monocytes, and macrophages; mouse anti-bovine CD4: +FITC; Bio-Rad, Hercules, USA), CD8 (co-receptor primarily expressed on cytotoxic T cells; mouse anti-bovine CD8: +RPE; Bio-Rad), CD14 (receptor expressed on monocytes and macrophages; mouse anti-bovine CD14: +RPE; Bio-Rad), and Article Title: Immune cells phenotype and bioenergetic measures in CD4 + T cells differ between high and low feed efficient dairy cows. Article Snippet: .. Next, PBMC were washed twice with PBS supplemented with 0.5% of BSA (PBS-BSA) and incubated for an additional 30 min at room temperature in the dark with 0.05 μg of anti-CD4 (MCA1653A700, clone CC8, Bio-Rad Laboratories, Inc., Hercules, CA), 3 μg of anti-CD8 (MCA837PE, clone CC63, Bio-Rad Laboratories, Inc., Hercules, CA), 0.1 μg of Bioprocessing:Article Title: Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves Article Snippet: Blood samples (100 μL) were incubated with VersaLyse (Beckman Coulter GmbH, Krefeld, Germany) for 20 min at room temperature and centrifuged at 250 × g for 5 min. .. Obtained cell pellets were washed twice by adding 700 μL phosphate-buffered saline (PBS) and subsequent centrifugation at 150 × g for 5 min. Staining of leukocytes was reached by adding monoclonal antibodies for marking CD4 (co-receptor primarily expressed on T-helper cells, monocytes, and macrophages; mouse anti-bovine CD4: +FITC; Bio-Rad, Hercules, USA), CD8 (co-receptor primarily expressed on cytotoxic T cells; mouse anti-bovine CD8: +RPE; Bio-Rad), CD14 (receptor expressed on monocytes and macrophages; mouse anti-bovine CD14: +RPE; Bio-Rad), and Article Title: Biocompatibility of Water-Dispersible Pristine Graphene and Graphene Oxide Using a Close-to-Human Animal Model: A Pilot Study on Swine. Article Snippet: The cells were transferred into 5 mL round bottom tubes (Corning) (1 × 106 PBMCs tube−1), stained with monoclonal antibodies specific for surface markers for 15 min at RT, and then washed with PBS containing 2% of FBS. .. The monocytes were differentiated using mouse anti-human CD14-PerCP-Cy5.5 (Tuk4; Miltenyi Biotec), whereas main lymphocyte populations were discriminated using the following monoclonal antibodies (moAb): Article Title: Biocompatibility of Water‐Dispersible Pristine Graphene and Graphene Oxide Using a Close‐to‐Human Animal Model: A Pilot Study on Swine Article Snippet: The cells were transferred into 5 mL round bottom tubes (Corning) (1 × 10 6 PBMCs tube −1 ), stained with monoclonal antibodies specific for surface markers for 15 min at RT, and then washed with PBS containing 2% of FBS. .. The monocytes were differentiated using mouse anti‐human CD14‐PerCP‐Cy5.5 (Tuk4; Miltenyi Biotec), whereas main lymphocyte populations were discriminated using the following monoclonal antibodies (moAb): mouse Incubation:Article Title: Immune cells phenotype and bioenergetic measures in CD4 + T cells differ between high and low feed efficient dairy cows. Article Snippet: .. Next, PBMC were washed twice with PBS supplemented with 0.5% of BSA (PBS-BSA) and incubated for an additional 30 min at room temperature in the dark with 0.05 μg of anti-CD4 (MCA1653A700, clone CC8, Bio-Rad Laboratories, Inc., Hercules, CA), 3 μg of anti-CD8 (MCA837PE, clone CC63, Bio-Rad Laboratories, Inc., Hercules, CA), 0.1 μg of Article Title: Supporting Information for: Unraveling clonal CD8 T cell expansion and discovery of novel factors in γ-herpesvirus-induced lymphomagenesis Article Snippet: .. Briefly, PBMCs were incubated with a monoclonal antibody cocktail containing Antibody Labeling:Article Title: Immune cells phenotype and bioenergetic measures in CD4 + T cells differ between high and low feed efficient dairy cows. Article Snippet: .. Next, PBMC were washed twice with PBS supplemented with 0.5% of BSA (PBS-BSA) and incubated for an additional 30 min at room temperature in the dark with 0.05 μg of anti-CD4 (MCA1653A700, clone CC8, Bio-Rad Laboratories, Inc., Hercules, CA), 3 μg of anti-CD8 (MCA837PE, clone CC63, Bio-Rad Laboratories, Inc., Hercules, CA), 0.1 μg of other:Article Title: Integrated metabolome and immunity analysis of immune-physiological responses in dairy cows under heat stress condition. Article Snippet: Briefly, the 210 PBMC fixed using 4% paraformaldehyde (PFA) for 20 min at 4°C and stained with direct fluorescence 211 conjugated antibodies; Article Title: Postnatal Epigenetic Alterations in Calves Persistently Infected with Bovine Viral Diarrhea Virus Article Snippet: , CD21 , |
