formaldehyde neutral buffer solution (Nacalai)

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Nacalai formaldehyde neutral buffer solution
Formaldehyde Neutral Buffer Solution, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+solution/buffer+formaldehyde+neutral+solution/pmc13259619-424-13-17
Average 86 stars, based on 1 article reviews

formaldehyde neutral buffer solution - by Bioz Stars, 2026-11

86/100 stars

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Staining:

Article Title: Mineralization-driven cellular dynamics and tissue remodeling in scaffold-free MC3T3-E1 spheroids.
Article Snippet: Bone mineralization is a critical process in bone tissue formation and remodeling, yet the underlying mechanisms in three-dimensional (3D) cellular environments remain unclear.. In this study, we fabricated scaffold-free spheroids using mouse osteoblast-like MC3T3-E1 cells and evaluated their long-term morphological and mechanical changes to elucidate the mineralization process.. The spheroids were cultured for up to 35 days in osteogenic medium, and their size reduction (53.2 % compared to 2-day spheroid), cellular viability, collagen accumulation, and mineral deposition were systematically analyzed.

Article Title: Confined migration induces non-lethal DNA damage in developing neurons.
Article Snippet: .. For haematoxylin and eosin (H&E) staining, anaesthetized mice were perfused with PBS followed by 10% formaldehyde neutral buffer solution (Nacalai, 37152- 51). ..

Incubation:

Article Title: Mpox virus OPG175 negatively regulates viral replication by controlling Wnt signaling
Article Snippet: .. At 3 dpi, the culture medium was removed, and cells were washed with PBS (+) once and fixed with a 10% formaldehyde neutral buffer solution (Cat# 37152-51, Nacalai Tesque) for 20 min. After permeabilization with 1% Triton X-100 (Cat# 35501-15, Nacalai Tesque) in PBS (−) for 5 min, cells were incubated with Mouse anti-Vaccinia virus L1 protein antibody (Cat#65-038, BioAcademia) (×1,000 dilution) at 37°C for 60 min. After washing with PBS (−), cells were incubated with goat anti-mouse IgG (H+L)-HRP (Cat# 5220-0341, KPL) (×3,000 dilution) at 37°C for 60 min. .. The foci of infected cells were visualized using a Peroxidase Stain 3,3’-diaminobenzidine (DAB) Kit (Cat# 25985-50, Nacalai Tesque) prepared with the Metal Enhancer for DAB Stain (Cat# 07388-24, Nacalai Tesque).

Article Title: Mpox virus OPG175 negatively regulates viral replication via controlling Wnt signaling
Article Snippet: .. At 3 dpi, the culture medium was removed, 475 and cells were washed with PBS (+) once and fixed with a 10% formaldehyde neutral 476 buffer solution (Cat# 37152-51, Nacalai Tesque) for 20 min. After permeabilization with 477 1% Triton X-100 (Cat# 35501-15, Nacalai Tesque) in PBS (−) for 5 min, cells were 478 incubated with Mouse anti-Vaccinia virus L1 protein antibody (Cat#65-038, 479 BioAcademia) (×1,000 dilution) at 37 °C for 60 min. After washing with PBS (−), cells 480 were incubated with goat anti-mouse IgG (H+L)-HRP (Cat# 5220-0341, KPL) (×3,000 481 dilution) at 37 °C for 60 min. .. The foci of infected cells were visualized using a Peroxidase 482 Stain 3,3’-diaminobenzidine (DAB) Kit (Cat# 25985-50, Nacalai Tesque) prepared with 483 the Metal Enhancer for DAB Stain (Cat# 07388-24, Nacalai Tesque).

Virus:

other:

Article Title: Posterior shift of Shh-Fgf signaling in axolotl limb regeneration drives sequential digit formation
Article Snippet: Counterstaining was performed with Hoechst 33342 (No. 346–07951; Wako Fujifilm).

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