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citrate antigen retrieval solution  (Boster Bio)


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    Structured Review

    Boster Bio citrate antigen retrieval solution
    Citrate Antigen Retrieval Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+solution/Citrate+Buffer+Powder/pm42911027-316-9-12
    Average 95 stars, based on 191 article reviews
    citrate antigen retrieval solution - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Suppression of microglial MEF2C by α-Synuclein is associated with neuroinflammation in a mouse model of Parkinson's disease and promotes degeneration In Vitro.
    Article Snippet: .. The immunofluorescence staining protocol was performed as follows: Mouse brain sections were first dewaxed in xylene and rehydrated through a graded ethanol series, followed by heat-mediated antigen retrieval in citrate buffer (BOSTER, Wuhan, China) for 30 min. After delineating the tissue sections with an immunohistochemical pen, endogenous peroxidase activity was quenched by incubating with 3% H2O2 solution at room temperature for 25 min in the dark, followed by blocking with 3% BSA for 30 min. ..

    Staining:

    Article Title: Suppression of microglial MEF2C by α-Synuclein is associated with neuroinflammation in a mouse model of Parkinson's disease and promotes degeneration In Vitro.
    Article Snippet: .. The immunofluorescence staining protocol was performed as follows: Mouse brain sections were first dewaxed in xylene and rehydrated through a graded ethanol series, followed by heat-mediated antigen retrieval in citrate buffer (BOSTER, Wuhan, China) for 30 min. After delineating the tissue sections with an immunohistochemical pen, endogenous peroxidase activity was quenched by incubating with 3% H2O2 solution at room temperature for 25 min in the dark, followed by blocking with 3% BSA for 30 min. ..

    Article Title: APX3330 reverses the immunosuppressive tumor microenvironment during colorectal carcinogenesis.
    Article Snippet: Observations and image capture were 385 conducted using an Olympus microscope. .. In the case of 386 immunohistochemical (IHC) staining, following deparaffinization, 387 antigen retrieval was performed utilizing either Citrate Antigen 388 Retrieval Solution (pH 6.0, Boster, Wuhan, China, #AR0024) or 389 EDTA Antigen Retrieval Solution (pH 8.0, MXB, China, MVS-0098), 390 in accordance with the specifications for the primary antibody. ..

    Article Title: APX3330 reverses the immunosuppressive tumor microenvironment during colorectal carcinogenesis
    Article Snippet: Observations and image capture were conducted using an Olympus microscope. .. In the case of immunohistochemical (IHC) staining, following deparaffinization, antigen retrieval was performed utilizing either Citrate Antigen Retrieval Solution (pH 6.0, Boster, Wuhan, China, #AR0024) or EDTA Antigen Retrieval Solution (pH 8.0, MXB, China, MVS-0098), in accordance with the specifications for the primary antibody. ..

    Article Title: GnIH-Induced Mitochondrial Dysfunction Lead to Oxidative Stress and Apoptosis in Thyroid Follicular Cells, Causing Hypothyroidism.
    Article Snippet: Thyroid disorders profoundly disrupt metabolism, development, growth, pubertal timing, and fertility in domestic animals.. Gonadotropin-inhibitory hormone (GnIH), a key inhibitory neuropeptide regulating reproductive function, has been implicated in metabolic dysfunction-associated infertility as well as thyroid dysfunction–related pubertal abnormalities.. These observations suggest potential crosstalk between GnIH and thyroid hormones (THs), positioning GnIH as a possible integrative regulator linking the hypothalamic-pituitary-thyroid (HPT) and hypothalamic-pituitary-gonadal (HPG) axes.

    Tissue:

    Article Title: Suppression of microglial MEF2C by α-Synuclein is associated with neuroinflammation in a mouse model of Parkinson's disease and promotes degeneration In Vitro.
    Article Snippet: .. The immunofluorescence staining protocol was performed as follows: Mouse brain sections were first dewaxed in xylene and rehydrated through a graded ethanol series, followed by heat-mediated antigen retrieval in citrate buffer (BOSTER, Wuhan, China) for 30 min. After delineating the tissue sections with an immunohistochemical pen, endogenous peroxidase activity was quenched by incubating with 3% H2O2 solution at room temperature for 25 min in the dark, followed by blocking with 3% BSA for 30 min. ..

    Article Title: Intratumoral Fusobacterium nucleatum promotes ferroptosis resistance and a disulfide-stress-associated vulnerability under glucose restriction.
    Article Snippet: Images were acquired using an FV3000 laser-scanning confocal microscope (FV3000, Olympus, Japan). .. Antigen retrieval was performed by heating tissue sections in citrate antigen-retrieval solution (Boster; Cat. No. AR0024) at 98 °C for 20 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide (Boster Biological Technology; Cat. No. AR1108) for 10 min. .. Sections were incubated overnight at 4 °C with anti-SLC7A11 antibody (1:1,000; Abcam, Cat. No. ab307601) or anti-GPX4 antibody (1:500; Servicebio, Cat. No. GB120010).

    Immunohistochemical staining:

    Article Title: Suppression of microglial MEF2C by α-Synuclein is associated with neuroinflammation in a mouse model of Parkinson's disease and promotes degeneration In Vitro.
    Article Snippet: .. The immunofluorescence staining protocol was performed as follows: Mouse brain sections were first dewaxed in xylene and rehydrated through a graded ethanol series, followed by heat-mediated antigen retrieval in citrate buffer (BOSTER, Wuhan, China) for 30 min. After delineating the tissue sections with an immunohistochemical pen, endogenous peroxidase activity was quenched by incubating with 3% H2O2 solution at room temperature for 25 min in the dark, followed by blocking with 3% BSA for 30 min. ..

    Article Title: APX3330 reverses the immunosuppressive tumor microenvironment during colorectal carcinogenesis.
    Article Snippet: Observations and image capture were 385 conducted using an Olympus microscope. .. In the case of 386 immunohistochemical (IHC) staining, following deparaffinization, 387 antigen retrieval was performed utilizing either Citrate Antigen 388 Retrieval Solution (pH 6.0, Boster, Wuhan, China, #AR0024) or 389 EDTA Antigen Retrieval Solution (pH 8.0, MXB, China, MVS-0098), 390 in accordance with the specifications for the primary antibody. ..

    Article Title: APX3330 reverses the immunosuppressive tumor microenvironment during colorectal carcinogenesis
    Article Snippet: Observations and image capture were conducted using an Olympus microscope. .. In the case of immunohistochemical (IHC) staining, following deparaffinization, antigen retrieval was performed utilizing either Citrate Antigen Retrieval Solution (pH 6.0, Boster, Wuhan, China, #AR0024) or EDTA Antigen Retrieval Solution (pH 8.0, MXB, China, MVS-0098), in accordance with the specifications for the primary antibody. ..

    Activity Assay:

    Article Title: Suppression of microglial MEF2C by α-Synuclein is associated with neuroinflammation in a mouse model of Parkinson's disease and promotes degeneration In Vitro.
    Article Snippet: .. The immunofluorescence staining protocol was performed as follows: Mouse brain sections were first dewaxed in xylene and rehydrated through a graded ethanol series, followed by heat-mediated antigen retrieval in citrate buffer (BOSTER, Wuhan, China) for 30 min. After delineating the tissue sections with an immunohistochemical pen, endogenous peroxidase activity was quenched by incubating with 3% H2O2 solution at room temperature for 25 min in the dark, followed by blocking with 3% BSA for 30 min. ..

    Article Title: Intratumoral Fusobacterium nucleatum promotes ferroptosis resistance and a disulfide-stress-associated vulnerability under glucose restriction.
    Article Snippet: Images were acquired using an FV3000 laser-scanning confocal microscope (FV3000, Olympus, Japan). .. Antigen retrieval was performed by heating tissue sections in citrate antigen-retrieval solution (Boster; Cat. No. AR0024) at 98 °C for 20 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide (Boster Biological Technology; Cat. No. AR1108) for 10 min. .. Sections were incubated overnight at 4 °C with anti-SLC7A11 antibody (1:1,000; Abcam, Cat. No. ab307601) or anti-GPX4 antibody (1:500; Servicebio, Cat. No. GB120010).

    Blocking Assay:

    Article Title: Suppression of microglial MEF2C by α-Synuclein is associated with neuroinflammation in a mouse model of Parkinson's disease and promotes degeneration In Vitro.
    Article Snippet: .. The immunofluorescence staining protocol was performed as follows: Mouse brain sections were first dewaxed in xylene and rehydrated through a graded ethanol series, followed by heat-mediated antigen retrieval in citrate buffer (BOSTER, Wuhan, China) for 30 min. After delineating the tissue sections with an immunohistochemical pen, endogenous peroxidase activity was quenched by incubating with 3% H2O2 solution at room temperature for 25 min in the dark, followed by blocking with 3% BSA for 30 min. ..

    Immunohistochemistry:

    Article Title: APX3330 reverses the immunosuppressive tumor microenvironment during colorectal carcinogenesis.
    Article Snippet: Observations and image capture were 385 conducted using an Olympus microscope. .. In the case of 386 immunohistochemical (IHC) staining, following deparaffinization, 387 antigen retrieval was performed utilizing either Citrate Antigen 388 Retrieval Solution (pH 6.0, Boster, Wuhan, China, #AR0024) or 389 EDTA Antigen Retrieval Solution (pH 8.0, MXB, China, MVS-0098), 390 in accordance with the specifications for the primary antibody. ..

    Article Title: APX3330 reverses the immunosuppressive tumor microenvironment during colorectal carcinogenesis
    Article Snippet: Observations and image capture were conducted using an Olympus microscope. .. In the case of immunohistochemical (IHC) staining, following deparaffinization, antigen retrieval was performed utilizing either Citrate Antigen Retrieval Solution (pH 6.0, Boster, Wuhan, China, #AR0024) or EDTA Antigen Retrieval Solution (pH 8.0, MXB, China, MVS-0098), in accordance with the specifications for the primary antibody. ..

    Article Title: GnIH-Induced Mitochondrial Dysfunction Lead to Oxidative Stress and Apoptosis in Thyroid Follicular Cells, Causing Hypothyroidism.
    Article Snippet: Thyroid disorders profoundly disrupt metabolism, development, growth, pubertal timing, and fertility in domestic animals.. Gonadotropin-inhibitory hormone (GnIH), a key inhibitory neuropeptide regulating reproductive function, has been implicated in metabolic dysfunction-associated infertility as well as thyroid dysfunction–related pubertal abnormalities.. These observations suggest potential crosstalk between GnIH and thyroid hormones (THs), positioning GnIH as a possible integrative regulator linking the hypothalamic-pituitary-thyroid (HPT) and hypothalamic-pituitary-gonadal (HPG) axes.

    Article Title: Emodin triggers cuproptosis to suppress hepatocellular carcinoma via SLC7A11/FDX1 axis
    Article Snippet: DAB Kit (G1212-200T) and Hematoxylin Staining Solution (G1004) were obtained from Servicebio (China). .. Immunohistochemistry Kit (SA1023) and Sodium Citrate Buffer (pH 6.0, AR0024) were purchased from Boster (China). .. Primary antibodies against SLC7A11 (DF12509), GPX4 (DF6701), and β-actin (T0022) were obtained from Affinity (USA), and the antibody against FDX1 (ab108257) from Abcam (UK).

    Incubation:

    Article Title: GnIH-Induced Mitochondrial Dysfunction Lead to Oxidative Stress and Apoptosis in Thyroid Follicular Cells, Causing Hypothyroidism.
    Article Snippet: Thyroid disorders profoundly disrupt metabolism, development, growth, pubertal timing, and fertility in domestic animals.. Gonadotropin-inhibitory hormone (GnIH), a key inhibitory neuropeptide regulating reproductive function, has been implicated in metabolic dysfunction-associated infertility as well as thyroid dysfunction–related pubertal abnormalities.. These observations suggest potential crosstalk between GnIH and thyroid hormones (THs), positioning GnIH as a possible integrative regulator linking the hypothalamic-pituitary-thyroid (HPT) and hypothalamic-pituitary-gonadal (HPG) axes.



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