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Valiant Co Ltd edta
Edta, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bme/2-Mercaptoethanol/pm42014705-358-25-33
Average 96 stars, based on 454 article reviews

edta - by Bioz Stars, 2026-09

96/100 stars

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RNA Extraction:

Article Title: Tissue resident memory T cells populate the human uveal tract.
Article Snippet: .. For RNA extraction, tissue was thawed and transferred to Buffer RLT (Qiagen) with 2-mercaptoethanol in a 2 ml soft tissue lysing kit (CK14; Precellys) and homogenised by shaking for 3 × 40 s at 6.5 m/s on a FastPrep-24 (MP Biomedicals) with samples cooled on ice for 1 min between shakes. .. After homogenisation, RNA was extracted using the RNeasy Plus Micro Kit (74,034; Qiagen) in accordance with the manufacturer protocol.

Article Title: Tissue resident memory T cells populate the human uveal tract
Article Snippet: .. For RNA extraction, tissue was thawed and transferred to Buffer RLT (Qiagen) with 2-mercaptoethanol in a 2 ml soft tissue lysing kit (CK14; Precellys) and homogenised by shaking for 3 × 40 s at 6.5 m/s on a FastPrep-24 (MP Biomedicals) with samples cooled on ice for 1 min between shakes. .. After homogenisation, RNA was extracted using the RNeasy Plus Micro Kit (74,034; Qiagen) in accordance with the manufacturer protocol.

Concentration Assay:

Article Title: Nup42 safeguards heat-induced mRNAs from nuclear condensation to support chaperone synthesis
Article Snippet: .. 4X NuPAGETM LDS Sample Buffer (Invitrogen, #NP0008) with 5% β-mercaptoethanol (MP Biomedicals, #190242) was added to each fraction for a final 1X concentration. .. Samples were then boiled at 95°C for 5 minutes and 5-10 μL was loaded onto a 3-8% NuPAGETM Tris-acetate gels (Thermo Fisher, #EA0375, #EA03755), and ran for 45-60 minutes at 150 V. Proteins were then transferred to nitrocellulose membranes (Biorad, #) Towbin buffer (25 mM Tris, 192 mM glycine, 20% w/v methanol) with wet-lab transfer apparatus (Biorad, #1704070), at 100 V for 1 hour in a 4°C cold-room.

Extraction:

Article Title: Highly mutagenic copying of telomeric circles promotes ALT establishment.
Article Snippet: .. Extraction and detection of telomeric circle DNA Extraction of circular DNA was performed from 1×109 cells by first incubating in 0.75 M sorbitol, 75 mM EDTA, and 0.7% 2-mercaptoethanol with 2.5 mg/mL Zymolyase (MP Biomedicals) at 37C for 90 minutes. .. Protoplasts were pelleted and treated with the Qiagen Plasmid Plus Midiprep Kit (Qiagen) as in88 with the following modifications: samples were centrifuged at 3,900×g for 10 minutes to remove cell debris before passage through a QIAfilter Midi cartridge, and washing with buffer ETR was omitted.

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edta (Valiant Co Ltd)

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