bme Search Results


β mercaptoethanol  (Bio-Rad)
96
Bio-Rad β mercaptoethanol
β Mercaptoethanol, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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drug 35s methionine perkin elmer  (Revvity)
91
Revvity drug 35s methionine perkin elmer
Drug 35s Methionine Perkin Elmer, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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cultrex pathclear bme gels  (R&D Systems)
94
R&D Systems cultrex pathclear bme gels
Cultrex Pathclear Bme Gels, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inserts 2 well  (R&D Systems)
94
R&D Systems inserts 2 well
Inserts 2 Well, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s methionine  (Revvity)
90
Revvity s methionine
S Methionine, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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matrigel domes  (R&D Systems)
94
R&D Systems matrigel domes
Human duodenal organoids were trypsinised into single cells and seeded onto culture plates pre-coated with <t>1:40</t> <t>Matrigel:PBS.</t> Cells were infected with S. flexneri ∆ ospc3 at an MOI of 100 for 4 h. CFUs in infected cells were enumerated ( a ), PI uptake was monitored using incucyte live cell imager and PI uptake was normalised per area confluency ( b ), images of monolayers taken 4 h post infection taken at 20x (i) scramble, uninfected; (ii) scramble, Shigella flexneri ∆ ospc3, (iii) GSDMD KD uninfected, (iv) GSDMD KD Shigella flexneri ∆ ospc3. Scale bars (indicting 200 micrometres) are depicted for each micrograph ( c ). Caspase-4 was measured in western blot of whole cell lysates ( d ), IL-18 secretion into the supernatant was measured by ELISA on cell free supernatants ( e ). A schematic of caspase-4 regulation ( f ). In ( f ) (i) LPS-induced caspase-4 activation (1) leads to activation of GSDMD and IL-18 (2). GSDMD pores lead to caspase-4 cleavage (3) and this stops IL-18 processing (4). In ( f )(ii), during conditions of reduced GSDMD –mediated plasma pore formation, caspase-4 activation (1) leads to IL-18 processing. A lack of efficient pore formation reduces inhibitory feedback on caspase-4 (3). This leads to continued activity of caspase-4 on IL-18 and IL-18 hypersecretion (4). All experiments are representative of at least 3 experiments. Data displayed as mean ± S.E.M. * p < 0.05, **** p < 0.001. Panel ( f ) created with Biorender.
Matrigel Domes, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
matrigel domes - by Bioz Stars, 2026-06
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cultrex bme cell invasion assay system  (R&D Systems)
94
R&D Systems cultrex bme cell invasion assay system
Human duodenal organoids were trypsinised into single cells and seeded onto culture plates pre-coated with <t>1:40</t> <t>Matrigel:PBS.</t> Cells were infected with S. flexneri ∆ ospc3 at an MOI of 100 for 4 h. CFUs in infected cells were enumerated ( a ), PI uptake was monitored using incucyte live cell imager and PI uptake was normalised per area confluency ( b ), images of monolayers taken 4 h post infection taken at 20x (i) scramble, uninfected; (ii) scramble, Shigella flexneri ∆ ospc3, (iii) GSDMD KD uninfected, (iv) GSDMD KD Shigella flexneri ∆ ospc3. Scale bars (indicting 200 micrometres) are depicted for each micrograph ( c ). Caspase-4 was measured in western blot of whole cell lysates ( d ), IL-18 secretion into the supernatant was measured by ELISA on cell free supernatants ( e ). A schematic of caspase-4 regulation ( f ). In ( f ) (i) LPS-induced caspase-4 activation (1) leads to activation of GSDMD and IL-18 (2). GSDMD pores lead to caspase-4 cleavage (3) and this stops IL-18 processing (4). In ( f )(ii), during conditions of reduced GSDMD –mediated plasma pore formation, caspase-4 activation (1) leads to IL-18 processing. A lack of efficient pore formation reduces inhibitory feedback on caspase-4 (3). This leads to continued activity of caspase-4 on IL-18 and IL-18 hypersecretion (4). All experiments are representative of at least 3 experiments. Data displayed as mean ± S.E.M. * p < 0.05, **** p < 0.001. Panel ( f ) created with Biorender.
Cultrex Bme Cell Invasion Assay System, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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cultrex 24 well basement membrane extract bme cell invasion assay  (R&D Systems)
93
R&D Systems cultrex 24 well basement membrane extract bme cell invasion assay
Human duodenal organoids were trypsinised into single cells and seeded onto culture plates pre-coated with <t>1:40</t> <t>Matrigel:PBS.</t> Cells were infected with S. flexneri ∆ ospc3 at an MOI of 100 for 4 h. CFUs in infected cells were enumerated ( a ), PI uptake was monitored using incucyte live cell imager and PI uptake was normalised per area confluency ( b ), images of monolayers taken 4 h post infection taken at 20x (i) scramble, uninfected; (ii) scramble, Shigella flexneri ∆ ospc3, (iii) GSDMD KD uninfected, (iv) GSDMD KD Shigella flexneri ∆ ospc3. Scale bars (indicting 200 micrometres) are depicted for each micrograph ( c ). Caspase-4 was measured in western blot of whole cell lysates ( d ), IL-18 secretion into the supernatant was measured by ELISA on cell free supernatants ( e ). A schematic of caspase-4 regulation ( f ). In ( f ) (i) LPS-induced caspase-4 activation (1) leads to activation of GSDMD and IL-18 (2). GSDMD pores lead to caspase-4 cleavage (3) and this stops IL-18 processing (4). In ( f )(ii), during conditions of reduced GSDMD –mediated plasma pore formation, caspase-4 activation (1) leads to IL-18 processing. A lack of efficient pore formation reduces inhibitory feedback on caspase-4 (3). This leads to continued activity of caspase-4 on IL-18 and IL-18 hypersecretion (4). All experiments are representative of at least 3 experiments. Data displayed as mean ± S.E.M. * p < 0.05, **** p < 0.001. Panel ( f ) created with Biorender.
Cultrex 24 Well Basement Membrane Extract Bme Cell Invasion Assay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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exogenous 35 s l methionine  (Revvity)
90
Revvity exogenous 35 s l methionine
Human duodenal organoids were trypsinised into single cells and seeded onto culture plates pre-coated with <t>1:40</t> <t>Matrigel:PBS.</t> Cells were infected with S. flexneri ∆ ospc3 at an MOI of 100 for 4 h. CFUs in infected cells were enumerated ( a ), PI uptake was monitored using incucyte live cell imager and PI uptake was normalised per area confluency ( b ), images of monolayers taken 4 h post infection taken at 20x (i) scramble, uninfected; (ii) scramble, Shigella flexneri ∆ ospc3, (iii) GSDMD KD uninfected, (iv) GSDMD KD Shigella flexneri ∆ ospc3. Scale bars (indicting 200 micrometres) are depicted for each micrograph ( c ). Caspase-4 was measured in western blot of whole cell lysates ( d ), IL-18 secretion into the supernatant was measured by ELISA on cell free supernatants ( e ). A schematic of caspase-4 regulation ( f ). In ( f ) (i) LPS-induced caspase-4 activation (1) leads to activation of GSDMD and IL-18 (2). GSDMD pores lead to caspase-4 cleavage (3) and this stops IL-18 processing (4). In ( f )(ii), during conditions of reduced GSDMD –mediated plasma pore formation, caspase-4 activation (1) leads to IL-18 processing. A lack of efficient pore formation reduces inhibitory feedback on caspase-4 (3). This leads to continued activity of caspase-4 on IL-18 and IL-18 hypersecretion (4). All experiments are representative of at least 3 experiments. Data displayed as mean ± S.E.M. * p < 0.05, **** p < 0.001. Panel ( f ) created with Biorender.
Exogenous 35 S L Methionine, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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ccl4  (R&D Systems)
92
R&D Systems ccl4
Human duodenal organoids were trypsinised into single cells and seeded onto culture plates pre-coated with <t>1:40</t> <t>Matrigel:PBS.</t> Cells were infected with S. flexneri ∆ ospc3 at an MOI of 100 for 4 h. CFUs in infected cells were enumerated ( a ), PI uptake was monitored using incucyte live cell imager and PI uptake was normalised per area confluency ( b ), images of monolayers taken 4 h post infection taken at 20x (i) scramble, uninfected; (ii) scramble, Shigella flexneri ∆ ospc3, (iii) GSDMD KD uninfected, (iv) GSDMD KD Shigella flexneri ∆ ospc3. Scale bars (indicting 200 micrometres) are depicted for each micrograph ( c ). Caspase-4 was measured in western blot of whole cell lysates ( d ), IL-18 secretion into the supernatant was measured by ELISA on cell free supernatants ( e ). A schematic of caspase-4 regulation ( f ). In ( f ) (i) LPS-induced caspase-4 activation (1) leads to activation of GSDMD and IL-18 (2). GSDMD pores lead to caspase-4 cleavage (3) and this stops IL-18 processing (4). In ( f )(ii), during conditions of reduced GSDMD –mediated plasma pore formation, caspase-4 activation (1) leads to IL-18 processing. A lack of efficient pore formation reduces inhibitory feedback on caspase-4 (3). This leads to continued activity of caspase-4 on IL-18 and IL-18 hypersecretion (4). All experiments are representative of at least 3 experiments. Data displayed as mean ± S.E.M. * p < 0.05, **** p < 0.001. Panel ( f ) created with Biorender.
Ccl4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bme  (Valiant Co Ltd)
96
Valiant Co Ltd bme
Human duodenal organoids were trypsinised into single cells and seeded onto culture plates pre-coated with <t>1:40</t> <t>Matrigel:PBS.</t> Cells were infected with S. flexneri ∆ ospc3 at an MOI of 100 for 4 h. CFUs in infected cells were enumerated ( a ), PI uptake was monitored using incucyte live cell imager and PI uptake was normalised per area confluency ( b ), images of monolayers taken 4 h post infection taken at 20x (i) scramble, uninfected; (ii) scramble, Shigella flexneri ∆ ospc3, (iii) GSDMD KD uninfected, (iv) GSDMD KD Shigella flexneri ∆ ospc3. Scale bars (indicting 200 micrometres) are depicted for each micrograph ( c ). Caspase-4 was measured in western blot of whole cell lysates ( d ), IL-18 secretion into the supernatant was measured by ELISA on cell free supernatants ( e ). A schematic of caspase-4 regulation ( f ). In ( f ) (i) LPS-induced caspase-4 activation (1) leads to activation of GSDMD and IL-18 (2). GSDMD pores lead to caspase-4 cleavage (3) and this stops IL-18 processing (4). In ( f )(ii), during conditions of reduced GSDMD –mediated plasma pore formation, caspase-4 activation (1) leads to IL-18 processing. A lack of efficient pore formation reduces inhibitory feedback on caspase-4 (3). This leads to continued activity of caspase-4 on IL-18 and IL-18 hypersecretion (4). All experiments are representative of at least 3 experiments. Data displayed as mean ± S.E.M. * p < 0.05, **** p < 0.001. Panel ( f ) created with Biorender.
Bme, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
bme - by Bioz Stars, 2026-06
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spheroid forming ecm solution  (R&D Systems)
94
R&D Systems spheroid forming ecm solution
Human duodenal organoids were trypsinised into single cells and seeded onto culture plates pre-coated with <t>1:40</t> <t>Matrigel:PBS.</t> Cells were infected with S. flexneri ∆ ospc3 at an MOI of 100 for 4 h. CFUs in infected cells were enumerated ( a ), PI uptake was monitored using incucyte live cell imager and PI uptake was normalised per area confluency ( b ), images of monolayers taken 4 h post infection taken at 20x (i) scramble, uninfected; (ii) scramble, Shigella flexneri ∆ ospc3, (iii) GSDMD KD uninfected, (iv) GSDMD KD Shigella flexneri ∆ ospc3. Scale bars (indicting 200 micrometres) are depicted for each micrograph ( c ). Caspase-4 was measured in western blot of whole cell lysates ( d ), IL-18 secretion into the supernatant was measured by ELISA on cell free supernatants ( e ). A schematic of caspase-4 regulation ( f ). In ( f ) (i) LPS-induced caspase-4 activation (1) leads to activation of GSDMD and IL-18 (2). GSDMD pores lead to caspase-4 cleavage (3) and this stops IL-18 processing (4). In ( f )(ii), during conditions of reduced GSDMD –mediated plasma pore formation, caspase-4 activation (1) leads to IL-18 processing. A lack of efficient pore formation reduces inhibitory feedback on caspase-4 (3). This leads to continued activity of caspase-4 on IL-18 and IL-18 hypersecretion (4). All experiments are representative of at least 3 experiments. Data displayed as mean ± S.E.M. * p < 0.05, **** p < 0.001. Panel ( f ) created with Biorender.
Spheroid Forming Ecm Solution, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Human duodenal organoids were trypsinised into single cells and seeded onto culture plates pre-coated with 1:40 Matrigel:PBS. Cells were infected with S. flexneri ∆ ospc3 at an MOI of 100 for 4 h. CFUs in infected cells were enumerated ( a ), PI uptake was monitored using incucyte live cell imager and PI uptake was normalised per area confluency ( b ), images of monolayers taken 4 h post infection taken at 20x (i) scramble, uninfected; (ii) scramble, Shigella flexneri ∆ ospc3, (iii) GSDMD KD uninfected, (iv) GSDMD KD Shigella flexneri ∆ ospc3. Scale bars (indicting 200 micrometres) are depicted for each micrograph ( c ). Caspase-4 was measured in western blot of whole cell lysates ( d ), IL-18 secretion into the supernatant was measured by ELISA on cell free supernatants ( e ). A schematic of caspase-4 regulation ( f ). In ( f ) (i) LPS-induced caspase-4 activation (1) leads to activation of GSDMD and IL-18 (2). GSDMD pores lead to caspase-4 cleavage (3) and this stops IL-18 processing (4). In ( f )(ii), during conditions of reduced GSDMD –mediated plasma pore formation, caspase-4 activation (1) leads to IL-18 processing. A lack of efficient pore formation reduces inhibitory feedback on caspase-4 (3). This leads to continued activity of caspase-4 on IL-18 and IL-18 hypersecretion (4). All experiments are representative of at least 3 experiments. Data displayed as mean ± S.E.M. * p < 0.05, **** p < 0.001. Panel ( f ) created with Biorender.

Journal: Communications Biology

Article Title: Gasdermin-D pores induce an inactivating caspase-4 cleavage that limits IL-18 production in the intestinal epithelium

doi: 10.1038/s42003-025-08183-9

Figure Lengend Snippet: Human duodenal organoids were trypsinised into single cells and seeded onto culture plates pre-coated with 1:40 Matrigel:PBS. Cells were infected with S. flexneri ∆ ospc3 at an MOI of 100 for 4 h. CFUs in infected cells were enumerated ( a ), PI uptake was monitored using incucyte live cell imager and PI uptake was normalised per area confluency ( b ), images of monolayers taken 4 h post infection taken at 20x (i) scramble, uninfected; (ii) scramble, Shigella flexneri ∆ ospc3, (iii) GSDMD KD uninfected, (iv) GSDMD KD Shigella flexneri ∆ ospc3. Scale bars (indicting 200 micrometres) are depicted for each micrograph ( c ). Caspase-4 was measured in western blot of whole cell lysates ( d ), IL-18 secretion into the supernatant was measured by ELISA on cell free supernatants ( e ). A schematic of caspase-4 regulation ( f ). In ( f ) (i) LPS-induced caspase-4 activation (1) leads to activation of GSDMD and IL-18 (2). GSDMD pores lead to caspase-4 cleavage (3) and this stops IL-18 processing (4). In ( f )(ii), during conditions of reduced GSDMD –mediated plasma pore formation, caspase-4 activation (1) leads to IL-18 processing. A lack of efficient pore formation reduces inhibitory feedback on caspase-4 (3). This leads to continued activity of caspase-4 on IL-18 and IL-18 hypersecretion (4). All experiments are representative of at least 3 experiments. Data displayed as mean ± S.E.M. * p < 0.05, **** p < 0.001. Panel ( f ) created with Biorender.

Article Snippet: Tissue was minced until able to be passed through a 1 mL pipette tip then incubated in 2 mg/mL collagenase at 37 o C for 40 min. Crypt units were dissociated by vigorous pipetting then washed 3x in PBS containing 10% FBS and plated into 40 μL Matrigel domes (Cultrex PathClear reduced growth factor BME, type 2; R&D Systems) in a 24 well plate.

Techniques: Infection, Western Blot, Enzyme-linked Immunosorbent Assay, Activation Assay, Clinical Proteomics, Activity Assay